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有山 恒
原稿種別: 本文
1965 年 32 巻 1 号 p.
1-7
発行日: 1965/07/25
公開日: 2018/02/09
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宮沢 滋, 和田 忠男, 佐々木 正明, 小山 睦子, 友野 ふき子
原稿種別: 本文
1965 年 32 巻 1 号 p.
8-14
発行日: 1965/07/25
公開日: 2018/02/09
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Some biological properties of thiamine monophosphate disulfide (TMPDS) was studied. TMPDS exerts approximately equal growth promoting effects for thiamine requiring organisms, L. fermenti 36 and rat. Partially purified thiaminases of bacilli could scarcely decomposed this compound. TMPDS is readily absorbed in man, rat, rabbit and guinea pig, by oral or parenteral administration and consequently higher content of thiamine and co-carboxylase were found in blood or organs of the animal in comparison to thiamine hydrochloride.
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水上 明彦, 熊倉 清次, 稲田 了, 赤坂 喜久治
原稿種別: 本文
1965 年 32 巻 1 号 p.
15-20
発行日: 1965/07/25
公開日: 2018/02/09
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Pharmacological effects of thiamine monophosphate disulfide (TMPDS) on acute toxicity of mice, the blood pressure of cats, hearts of dogs, guinea pigs and toads, and the movement of small intestines of guinea pigs and rabbits were studied in comparison with those of thiamine hydrochloride. The experimental results showed that the dynamic pharmacological effects of TMPDS on them were less remarkable than those of thiamine hydrochloride.
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水上 明彦, 鈴木 善雄, 稲津 佳彦, 片海 晟五, 加藤 利治
原稿種別: 本文
1965 年 32 巻 1 号 p.
21-29
発行日: 1965/07/25
公開日: 2018/02/09
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Chronic toxicity tests were made on thiamine monophophate disulfide (TMPDS). TMPDS was administered subcutaneously, 5.16,51.6 and 412.8 mg/kg/day for 12 weeks in Wistar-Imamichi rats and 51.6 mg/kg/day for 4 weeks in dogs. No effect on the general symptoms and clinical and histo-pathology was observed.
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水上 明彦, 鈴木 善雄, 島田 精一, 須藤 征児
原稿種別: 本文
1965 年 32 巻 1 号 p.
30-50
発行日: 1965/07/25
公開日: 2018/02/09
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The present authors investigated the influences of the administration of a thiamine derivative as thiamine monophosphate disulfide (TMPDS) on the fetus in pregnant experimental animals and its youngs, according to the test method of the influences of new drug on fetus. TMPDS was given intraperitoneally 20mg per kg (small dose group), 100 mg per kg (intermediate dose group), 200 mg per kg (large dose group) during 7〜12th day in pregnant ICR mice and 9〜14th day in pregnant Wistar-Imamichi rat, every day. TMPDS results in malformations such as cleft plate, curve of tail, etc., in mice at large dose. Malformation could not be observed in rats. Nevertheless, when it was given within the range of 10 to 100 times of proposed therapeutic dose, the administration of TMPDS does not have any inhibitory effect on the development of fetus in pregnant mice and rats or their youngs.
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藤田 公司, 虫鹿 好孝
原稿種別: 本文
1965 年 32 巻 1 号 p.
51-55
発行日: 1965/07/25
公開日: 2018/02/09
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Thiamine monophosphate disulfide (TMPDS) is obtained by the oxidation of thiamine monophosphate in alkaline solution. As oxidizing agents halogens, hydrogenperoxide and ferricyanide can be used. TMPDS is soluble in water, slightly soluble in methanol and ethanol, insoluble in other organic solvents (ether, benzene, acetone). Polymorphisms of TMPDS are shown in Tab. 2 and Fig. 2. Metallic salts of TMPDS are shown in Tab. 3.
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増川 健二, 古崎 喜市郎, 辻 正男, 沢田 弘
原稿種別: 本文
1965 年 32 巻 1 号 p.
56-62
発行日: 1965/07/25
公開日: 2018/02/09
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Investigation was made on the physical and chemical properties and the quantitative determination of thiamine monophosphate disulfide (TMPDS). Ultraviolet absorption spectrum of its aquenous solution is variable with changes in pH, and the isosbestic points are 239 and 277 mμ. Dissociation constants obtained by the titration curve are pKa_1 5.90,pKa_2 7.55,pKb 11.93 and isoelectric point was calculated as pH 3.96. TMPDS is insoluble in organic solvents, but soluble in water, and the solubility decreases to the minimum value at pH 4. Rf is 0.47,when a developing solvent consisted of pyridine, acetic acid, water is used, by the thin layer chromatography. Anhydrous TMPDS is hygroscopic, but its hydrates (4 to 8H_2O) are stable. An aqueous solution of TMPDS is stable for heating at 60〜7O℃ in acidic enviroments, particulary at pH 4,while it is unstable in alkaline solution. TMPDS is reduced quantitatively with cysteine in a pH 13.0 at 35℃ for 15 minutes. Thiamine monophosphate (TMP) thus produced was determined by ultraviolet absorption of flurometric method, since it is also converted to thiochrome phosphate with cyanogen bromide and alkali. When TMPDS, thiamine and TMP are contained together in blood, the total thiamine is satitfactorily determined by usual thiochrome method after cysteine-Takadiastase hydrolysis.
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能勢 尚志, 安田 晃三, 中間 元隆, 足立 史郎, 甲和 良夫, 山田 茂樹, 石川 節子, 千畑 一郎
原稿種別: 本文
1965 年 32 巻 1 号 p.
63-69
発行日: 1965/07/25
公開日: 2018/02/09
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Biological and pharmacological studies were made on thiamine monophosphate disulfide (TMPDS). In thiamine deficient rats, TMPDS exerted the thiamine activity approximately equivalent to that of thiamine hydrochloride. Administration of TMPDS to dogs resulted in long retention of thiamine in high blood concentration together with the increased blood cocarboxylase activity lasting for a long duration. TMPDS exhibited the analgesic activity nearly equal to that of aminopyrine. In rabbits TMPDS produced moderate falls of blood pressure with little influence on respiration. Myocardial activity was depressed in the heart isolated from frogs. Peristaltic movement was stimulated in the intact intestine of rabbits. Acute toxicity in mice was much lower than that of thiamine hydrochloride. TMPDS was definitely resistant to thiaminase I and II produced by Bacillus thiaminolyticus and B. aneurinolyticus.
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堀 正樹, 中山 良夫, 野口 祐三, 甲和 良夫
原稿種別: 本文
1965 年 32 巻 1 号 p.
70-76
発行日: 1965/07/25
公開日: 2018/02/09
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Safety evaluation of thiamine monophosphate disulfide (TMPDS) was made by chronic toxicity and teratological tests. In the chronic toxicity test, TMPDS was administered to rats intraperitoneally in doses of 100 and 50 mg/kg daily for six months. Between the control and the animals injected with TMPDS was observed no difference in the body weight gain, food consumption, hematologic findings, organ weights and histologic findings. In the teratological test, 500,100 and 50 mg/kg of TMPDS were given intraperitoneally to pregnant rats and mice every day from the 7th to the 14th day of gestation. No influence of TMPDS was observed on both fetuses and newborns obtained from the mother animals.
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壬生 敦郎
原稿種別: 本文
1965 年 32 巻 1 号 p.
77-81
発行日: 1965/07/25
公開日: 2018/02/09
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It has been considered by the investigation of J. Reed et al. that a protein-bound form was the active one of lipoic acid for oxidative decarboxylation of α-ketoacid such as pyruvic or α-ketoglutaric acid. Therefore, the author studied the effect of protein composition in the diet on the biosynthesis of the protein-bound lipoic acid using Nose and Mori's method. The incorporation rate per mg N in liver mitochondrial protein (LMP) in all groups was elevated during the whole feeding period. The incorporation rate per mg N in LMP in low protein diet was higher than that in standard diet and was lower than that in protein-free diet. Therefore, it was concluded that in the low protein and protein-free diets the formation of the protein relating with the lipoic acid-activating system was lowered than that of the other liver protein. When ^<35>S-labelled lipoic acid was injected, the incorporation rate per mg N in LMP was remarkably elevated until the 1st hour but rapidly decreased thereafter.
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壬生 敦郎
原稿種別: 本文
1965 年 32 巻 1 号 p.
82-85
発行日: 1965/07/25
公開日: 2018/02/09
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Experimental studies were performed on the effect of tryptophan and lysine deficiency in the diet for the biosynthesis of the protein-bound lipoic acid using Nose and Mori's method. Rats were divided into three groups : standard diet, tryptophan deficient- and lysine deficient groups. No remarkable change in the weights of body and liver in all groups was noted. The incorporation rate per mg N in liver mitochondrial protein was highest in the standard diet group, followed by lysine deficient- and tryptophan deficient groups. When lipoic acid-^<35>S was injected the incorporation rate was elevated until first hour in all groups, but the later lowering was faster in lysine- and tryptophan-deficient groups than standard diet group.
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壬生 敦郎
原稿種別: 本文
1965 年 32 巻 1 号 p.
86-89
発行日: 1965/07/25
公開日: 2018/02/09
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Experimental studies was performed on the effect of polished rice as protein source in the diet on biosynthesis of protein-bound lipoic acid using Nose and Mori's method. Rats were divided into seven groups : polished rice standard group, lysine-, methionine-, tryptophan-, threonine- and phenylalanine-deficient groups and polished rice groups. The weight gains of body and liver were the following order : standard group or methionine-deficient, phenylanine- and tryptophan-deficient groups, lysine- and threonine deficient groups and polished rice group. The incorporation rate per mg N in liver mitochondrial protein (LMP) decreased in polished rice group and tryptophan- and threonine-deficient groups, and the rates in methionine-, lysine- or phenylalanine deficient groups were the same as that of standard group. When ^<35>S-labeled lipoic acid was injected, the incorporation rate per mg N in LMP was remarkably increased until 30 minutes, but in the polished rice group the increasing rate was slower than standard group, and in threonine-deficient group the decreasing was faster than others.
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高橋 正侑, 檜垣 宮都, 鈴木 隆雄, 佐橋 佳一
原稿種別: 本文
1965 年 32 巻 1 号 p.
90-93
発行日: 1965/07/25
公開日: 2018/02/09
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In the previous papers, the authors studied the intimate relationship in chicks between vitamin D_3 metabolism and active sulfate metabolism, and activities of vitamin D_3 in active sulfate metabolism was demonstrated using ^<35>S-labeled sulfate. Recently, further investigation was performed for the chemical synthesis of vitamin D_2-sulfate, and its properties were carefully tested. Finally, the sulfate was isolated from the rabbit urine loaded with vitamin D_2.
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井口 定男, 山本 孫兵衛, 青山 敏信
原稿種別: 本文
1965 年 32 巻 1 号 p.
94-98
発行日: 1965/07/25
公開日: 2018/02/09
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Gas chromatographic seperation by direct injection of a mixture of dihydrolipoic acid, lipoic acid, and lipoamide was examined using the column packing of 15% diethyleneglycol succinate polyester on Chromosorb W at 220 to 230℃ with carrier gas of nitrogen and the flame ionization detector. Calibration curves for the determination were obtained using di-n-octyl phthalate for lipoic acid and benzyl n-butyl phthalate for lipoamide as the internal standard, respectively. Lipoic acid and lipoamide in the pharmaceuticals were readily determined from the chloroform extracts with mean recovery±standard deviation of 98.9±2.9% and 98.8±2.7%, respectively. Procedures are presented in detail.
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竹内 勝, 麻生 和雄, 川瀬 健二, 市川 浩, 塩見 輝雄
原稿種別: 本文
1965 年 32 巻 1 号 p.
99-106
発行日: 1965/07/25
公開日: 2018/02/09
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Because of the recent use of a large quantity of vitamin C for pigment anomaly, a restudy on the excretion in urine of vitamin C was conducted laying stress on oxalic acid, its metabolites. Furthermore an investigation on the increase of oxalate calculus by experimentally inserting calculus in bladder of rats and administering vitamin C to them was also made. When a large amount of vitamin C was administered, in most cases (approximately 80 percent) vitamin C was excreted mainly as dehydroascorbic acid in urine. Remarkable increase of urinary oxalic acid was not observed with oral administration or intravenous injection of 3 g of ascorbic acid per day as compared with control, but with 9g oral administration oxalic acid increased by 20 to 30 mg. Since 3,4-diketogulonic acid increases according to increased doses of vitamin C, it can be said that decomposition of dehydroascorbic acid to 3,4-diketogulonic acid was accelerated, but it seems that further oxidation to oxalic acid would not be so conspicuous. The amount of radioactive oxalic acid in urine, after the administration of ascorbic acid-1^<14>C, was 1 to 1.5 percent of doses in animals and 0.16 percent in men or 0.3 to 0.7 mg or 3 mg, respectively. In rats which were administered with 500 μg per day for 17 days by intramuscular injection, increase of the calculus was noted. In this case, oxalic acid in calculus was 0.733 mg as compared with 0.326 mg in the control group.
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山田 雅子
原稿種別: 本文
1965 年 32 巻 1 号 p.
107-110
発行日: 1965/07/25
公開日: 2018/02/09
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^<14>C-(2)-Riboflavin tetrabutyrate, synthesized from ^<14>C-(2)-riboflavin and butyric anhydride, was administered to the normal rats by injection or per os. At 24 hours after the administration, the radioactivities were measured in the FAD, FMN, and riboflavin fractions extracted from the liver, kidney, heart, and small intestine of the rats. The results showed that the largest part of the total radioactivity was found in the flavins of the liver followed by the kidney and the small intestine. The trace of the radioactivity was found in the flavins of the heart. From these results, it was concluded that the riboflavin moiety of the butyrate administered by injection or per os can be incorporated into FMN and FAD of these organs.
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川村 正彦
原稿種別: 本文
1965 年 32 巻 1 号 p.
111-115
発行日: 1965/07/25
公開日: 2018/02/09
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Studies of the urinary excretion of tryptophan metabolites in normal subjects have been reported by many investigators, but comparison of these results is difficult because of differences in body-weights, the doses of tryptophan and experimental methods. The metabolism of tryptophan in 20 normal children was studied by the present author using ion exchange column chromatography. The urinary excretion of tryptophan metabolites in normal children before and after the administration of 2g of L-tryptophan was as follows (μmoles per kg per 24hrs). Tryptophan 0 and 2.7,kynurenine 1.4 and 6.2,kynurenic acid 1.0 and 3.3,xanthurenic acid 0.3 and 1.3,anthranilic acid 0.5 and 2.9,pyridone 1.8 and 3.7. The conversion rate of given tryptophan was 3.23%.
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川村 正彦
原稿種別: 本文
1965 年 32 巻 1 号 p.
116-120
発行日: 1965/07/25
公開日: 2018/02/09
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In some patients with renal glycosuria, the lowerings of serum riboflavin and vitamin B_6 values are recognized. Therefore, the present author studied the tryptophan metabolism of children with renal glycosuria. In 30 patients, all of 3 cases with continuous renal glycosuria and 8 out of 27 cases with intermittent renal glycosuria showed abnormal urinary excretion of tryptophan metabolites. These abnormalities were classified in 5 types and it was suggested that the causes of the abnormalities were based on the deficiency of riboflavin or of vitamin B_6 or the lowering of the activities of enzymes, having relations to these vitamins. In the patients with continuous renal glycosuria, these abnormalities became normal by the administration of riboflavin and pyridoxal phosphate, but the glycosuria remained. On the other hand, in the patient with intermittent glycosuria, urinary glucose was disappeared by riboflavin. It is suggested the renal glycosuria has some relations not only with glucose metabolism but also with amino acids metabolism.
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水口 茂
原稿種別: 本文
1965 年 32 巻 1 号 p.
121-124
発行日: 1965/07/25
公開日: 2018/02/09
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Five strains of anaerobic thiaminolytic bacilli were isolated from the feces of patients suffering from various diseases without thiamine deficiency. These strains had the almost same morphological, biological and chemical properties as those of 3 standard strains of Clostridium thiaminolyticum Kimura et Liao, previously described. They had a little difference in the biological and chemical properties, compared with those of two strains of Clostridium sporogenes. On the contrary, at least 3 strains of these new isolates could be rather clearly distinguished from Clostridium sporogenes by the cross agglutination test using each of their heated antigens and rabbit antisera.
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竹内 勝, 麻生 和雄, 川瀬 健二, 市川 浩, 塩見 輝男
原稿種別: 本文
1965 年 32 巻 1 号 p.
125-
発行日: 1965/07/25
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竹内 勝, 麻生 和雄, 川瀬 健二
原稿種別: 本文
1965 年 32 巻 1 号 p.
125-
発行日: 1965/07/25
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稲垣 長典, 大泉 久美子
原稿種別: 本文
1965 年 32 巻 1 号 p.
125-126
発行日: 1965/07/25
公開日: 2018/02/09
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井上 真由美, 昼間 善継, 勝田 清和
原稿種別: 本文
1965 年 32 巻 1 号 p.
126-
発行日: 1965/07/25
公開日: 2018/02/09
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松永 仁
原稿種別: 本文
1965 年 32 巻 1 号 p.
126-
発行日: 1965/07/25
公開日: 2018/02/09
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工藤 祐三, 玉沢 佳巳, 本多 能久
原稿種別: 本文
1965 年 32 巻 1 号 p.
126-127
発行日: 1965/07/25
公開日: 2018/02/09
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吉利 和, 柴田 長夫, 安田 和人, 山本 学
原稿種別: 本文
1965 年 32 巻 1 号 p.
127-
発行日: 1965/07/25
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竹内 勝, 麻生 和雄, 近藤 省三
原稿種別: 本文
1965 年 32 巻 1 号 p.
127-128
発行日: 1965/07/25
公開日: 2018/02/09
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竹内 勝, 麻生 和雄, 内海 滉, 小林 隆哉
原稿種別: 本文
1965 年 32 巻 1 号 p.
128-
発行日: 1965/07/25
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鈴置 二郎, 西川 浩平, 松尾 隆夫, 田中 文彦
原稿種別: 本文
1965 年 32 巻 1 号 p.
128-
発行日: 1965/07/25
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原田 清, 大良 勇, 斉藤 一文字, 内海 勇
原稿種別: 本文
1965 年 32 巻 1 号 p.
128-129
発行日: 1965/07/25
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川北 兵蔵, 兼松 弘, 新谷 勲, 今村 正男
原稿種別: 本文
1965 年 32 巻 1 号 p.
129-
発行日: 1965/07/25
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川北 兵蔵, 兼松 弘, 新谷 勲, 今村 正男
原稿種別: 本文
1965 年 32 巻 1 号 p.
129-
発行日: 1965/07/25
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太幡 利一
原稿種別: 本文
1965 年 32 巻 1 号 p.
130-
発行日: 1965/07/25
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太幡 利一, 荒川 武夫
原稿種別: 本文
1965 年 32 巻 1 号 p.
130-
発行日: 1965/07/25
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馬場 春夫, 平山 昌子
原稿種別: 本文
1965 年 32 巻 1 号 p.
130-
発行日: 1965/07/25
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飯田 民治
原稿種別: 本文
1965 年 32 巻 1 号 p.
130-131
発行日: 1965/07/25
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須藤 浩, 内田 仙二, 坂本 広司
原稿種別: 本文
1965 年 32 巻 1 号 p.
131-
発行日: 1965/07/25
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晴山 信一, 渡辺 真成, 小柳 達男, 村松 縁, 三浦 由雄, 伊藤 敏夫
原稿種別: 本文
1965 年 32 巻 1 号 p.
131-
発行日: 1965/07/25
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川崎 近太郎, 伊藤 誉志男
原稿種別: 本文
1965 年 32 巻 1 号 p.
131-132
発行日: 1965/07/25
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泉 寛治, 繁田 幸男, 谷和 光彦
原稿種別: 本文
1965 年 32 巻 1 号 p.
132-
発行日: 1965/07/25
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小川 俊太郎, 越本 朱美, 守田 実, 西浦 加恵子
原稿種別: 本文
1965 年 32 巻 1 号 p.
132-133
発行日: 1965/07/25
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高橋 忠男, 安田 晃三, 甲和 良夫, 藤原 充雄
原稿種別: 本文
1965 年 32 巻 1 号 p.
133-
発行日: 1965/07/25
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池田 幸
原稿種別: 本文
1965 年 32 巻 1 号 p.
133-
発行日: 1965/07/25
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舛重 正一, 鈴木 隆雄, 佐橋 佳一
原稿種別: 本文
1965 年 32 巻 1 号 p.
133-134
発行日: 1965/07/25
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岩島 昭夫, 川崎 尚, 能勢 善嗣
原稿種別: 本文
1965 年 32 巻 1 号 p.
134-
発行日: 1965/07/25
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満田 久輝, 河合 文雄, 橋谷 義人
原稿種別: 本文
1965 年 32 巻 1 号 p.
134-
発行日: 1965/07/25
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植松 寿樹, 中村 美代子, 能勢 善嗣
原稿種別: 本文
1965 年 32 巻 1 号 p.
135-
発行日: 1965/07/25
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中村 美代子, 能勢 善嗣
原稿種別: 本文
1965 年 32 巻 1 号 p.
135-
発行日: 1965/07/25
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遊佐 孝
原稿種別: 本文
1965 年 32 巻 1 号 p.
135-136
発行日: 1965/07/25
公開日: 2018/02/09
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吉井 善作, 原田 肥育, 二五田 公俊, 渋武 真知子, 林 良二
原稿種別: 本文
1965 年 32 巻 1 号 p.
136-
発行日: 1965/07/25
公開日: 2018/02/09
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