巻号一覧
48 巻, 9 号
選択された号の論文の20件中1～20を表示しています
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山本 尚三原稿種別: 本文
1974 年 48 巻 9 号 p. 419-432
発行日: 1974/09/25
公開日: 2018/03/02
ジャーナル フリーThree pseudomonad monooxygenases, salicylate hydroxylase, imidazoleacetate hydroxylase and lysine oxygenase, were purified and identified to be flavoproteins. Their prosthetic flavin was flavin adenine dinucleotide. Since neither iron nor copper was detected in a significant quantity, the flavin seemed to be a sole prosthetic group of these enzymes. These and other flavooxygenases comprise a major new group of flavoproteins. Lysine oxygenase catalyzed an oxygenative decarboxylation of lysine producing an acid amide. Under anaerobic conditions, however, the enzyme showed a dehydrogenation of lysine and produced a corresponding α-keto acid. Upon the modification of the enzyme protein by sulfhydryl blocking reagents, the enzyme now catalyzed an oxidative deamination of lysine producing an α-keto acid and hydrogen peroxide. Furthermore, certain analogues such as ornithine and γ-methyllysine underwent an oxidative deamination. α-Monoamino acids were also oxidized, but only when alkylamines were present. Thus, an oxidase activity was demonstrated by the modification of either the enzyme or the substrate. The significance of this oxidase activity is discussed in terms of the oxygen activation by the flavin.抄録全体を表示PDF形式でダウンロード (1557K) -
田中 直義, 盛永 宏太郎, 舛重 正一, 前川 昭男, 鈴木 隆雄, 佐橋 佳一原稿種別: 本文
1974 年 48 巻 9 号 p. 433-437
発行日: 1974/09/25
公開日: 2018/03/02
ジャーナル フリーThe growth of Saccharomyces carlsbergensis was studied when thiamine in the vitamin B_5 assay basal medium, was replaced by various thiamine-related compounds. The results were as follows ; 1) No difference was found in the growth in the medium containing thiamine diphosphate, thiamine palmitate, thiamine isovalerate, thiamine propyldisulfide, thiamine disulfide, or the mixture of 2-methyl-4-amino-5-aminomethylpyrimidine and 4-methyl-5(β-hydroxyethyl) thiazole, as compared with thiamine respectively. But the yeast growth in the medium containing O, S-dicarbethoxythiamine was lower than that in the medium containing thiamine, while, in the medium which contained ethylthiamine or chlorothiamine instead of thiamine, the state of the growth showed a high blank. 2) Ethylthiamine or chlorothiamine had no effect on the growth of thiamine-requiring Lactobacillus viridescens.抄録全体を表示PDF形式でダウンロード (511K) -
上田 文雄, 東 正, 鮎川 好一原稿種別: 本文
1974 年 48 巻 9 号 p. 439-445
発行日: 1974/09/25
公開日: 2018/03/02
ジャーナル フリーAfter estimating the swelling properties and solvent regain. values of hydroxypropylated Sephadex G-50 (organophilic dertran gel), behavior of fat-soluble vitamins on the column was examined. The available fractionation range for gel filtration utilizing hydroxypropylated Sephadex G-50 as a bed and chloroform as an eluting solvent was found to be between 200 and 10,000 in the molecular weight, but the results on the separation of fat-soluble vitamins were not better than the previous reports (Vitamins, 39,176 (1969), 40,309 (1969)) using Sephadex LH-20. Therefore, the method would be available only for separation of compounds having large differences in their molecular size.抄録全体を表示PDF形式でダウンロード (609K) -
上島 俊輔, 長谷川 忠男, 鈴木 隆雄, 稲葉 繁原稿種別: 本文
1974 年 48 巻 9 号 p. 447-452
発行日: 1974/09/25
公開日: 2018/03/02
ジャーナル フリー^3H-Vitamin D_2 (^3H-D_2) and ^3H-vitamin D_2-sulfate (^3H-D_2-sulfate) were prepared by Wilzbach method and D_2-sulfate binding proteins from the rabbit intestinal mucosa and plasm were investigated in vitro. The intestinal mucosa proteins reacted with ^3H-D_2 or ^3H-D_2-sulfate, on its gel filtration using Sephadex G-200 column (1.6×70cm), were separated into six protein peaks respectively. Peak 1 equivalent to the void volume was that bound with ^3H-D_2 or ^3H-D_2-sulfate. After pronase treatment of the intestinal mucosa proteins, their binding ability with ^3H-D_2-sulfate were decreased markedly. The disk get electrophoresis of ^3H-D_2-sulfate binding proteins in the peak 1 fraction was also carried out ; all the three bands on the gel stained with amide black showed radioactivity. These results suggest that D_2-sulfate can bind with intestinal mucosa proteins to be absorbed from the intestine. When the rabbit plasm binding proteins with ^3H-D_2-sulfate were separated by gel filtration using Sephadex G-200 column, six protein peaks were observed ; the protein in peak 3 fraction was shown to be that bound with either ^3H-D_2 or ^3H-D_2-sulfate respectively.抄録全体を表示PDF形式でダウンロード (616K) -
二五田 公俊原稿種別: 本文
1974 年 48 巻 9 号 p. 453-462
発行日: 1974/09/25
公開日: 2018/03/02
ジャーナル フリーA mutant strain of Escherichia coli, O8-442,requiring lipoic acid (LiA) for both aerobic and anaerobic growth in a minimal medium with glucose as a sole carbon source was isolated. The requirment for LiA could be replaceable by either acetate+succinate or acetate+lysine+methionine under aerobic conditions, but anaerobically it grew on either acetate or succinate alone. Pyruvate markedly stimu1ated.the respiration of the mutant cells grown aerobically on LiA and the addition of LiA did not cause a further stimulation. Conversely, results obtained with the cells grown aerobically on acetate+ lysine+methionine was quite different ; the degree of the pyruvate-oxidizing activity was very low but the activity was fully restored by the addition of LiA. In the same system, lipoamide (LiANH_2) was found to have no activity for the respiration of the mutant cells, while it was more active than LiA when extracts prepared from the mutant cells were used. These results suggest that the de novo synthesis of LiA is blocked in the mutant strain. A permeability barrier appears to be involved in the utilization of LiANH_2.抄録全体を表示PDF形式でダウンロード (1133K) -
田原 京子, 松岡 繁, 大浜 宏文原稿種別: 本文
1974 年 48 巻 9 号 p. 463-
発行日: 1974/09/25
公開日: 2018/03/14
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鈴木 襄, 岩井 和夫原稿種別: 本文
1974 年 48 巻 9 号 p. 463-
発行日: 1974/09/25
公開日: 2018/03/14
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Upendra Prasad C, M.Bose S原稿種別: 本文
1974 年 48 巻 9 号 p. 463-464
発行日: 1974/09/25
公開日: 2018/03/14
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上原 悌次郎, 徳重 正信原稿種別: 本文
1974 年 48 巻 9 号 p. 464-
発行日: 1974/09/25
公開日: 2018/03/14
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5.Thiosemicarbazide(抗ビタミンB_6剤)に対する感受性と脳の発達(JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY : 掲載論文要旨)山下 洵子原稿種別: 本文
1974 年 48 巻 9 号 p. 464-
発行日: 1974/09/25
公開日: 2018/03/14
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V.Khandwekar P, Nath N, C.Nath M原稿種別: 本文
1974 年 48 巻 9 号 p. 464-465
発行日: 1974/09/25
公開日: 2018/03/14
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Migasena Panata, I.Thurnham D, Pongpaew Praneet, Hongthong Kanjana, Ha ...原稿種別: 本文
1974 年 48 巻 9 号 p. 465-
発行日: 1974/09/25
公開日: 2018/03/14
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中村 征夫, 安本 教伝, 満田 久輝原稿種別: 本文
1974 年 48 巻 9 号 p. 465-
発行日: 1974/09/25
公開日: 2018/03/14
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遠藤 章二, 加守 和美, 新山 喜昭原稿種別: 本文
1974 年 48 巻 9 号 p. 465-
発行日: 1974/09/25
公開日: 2018/03/14
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光永 俊郎原稿種別: 本文
1974 年 48 巻 9 号 p. 465-466
発行日: 1974/09/25
公開日: 2018/03/14
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飯守 三郎, 道本 和子, 壷井 輝子原稿種別: 本文
1974 年 48 巻 9 号 p. 467-
発行日: 1974/09/25
公開日: 2018/03/02
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美濃 真, 畑 千恵子原稿種別: 本文
1974 年 48 巻 9 号 p. 468-
発行日: 1974/09/25
公開日: 2018/03/02
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鈴木 隆雄, 舛重 正一, 田所 忠弘, 菊永 茂司原稿種別: 本文
1974 年 48 巻 9 号 p. 468-469
発行日: 1974/09/25
公開日: 2018/03/02
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小林 正, 康村 満枝原稿種別: 本文
1974 年 48 巻 9 号 p. 469-
発行日: 1974/09/25
公開日: 2018/03/02
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小林 正, 吉本 佐雅子, 康村 満枝原稿種別: 本文
1974 年 48 巻 9 号 p. 469-470
発行日: 1974/09/25
公開日: 2018/03/02
ジャーナル フリーPDF形式でダウンロード (232K)
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