巻号一覧
36 巻 (1967)
- 6 号 p. 487-
- 5 号 p. 367-
- 4 号 p. 293-
- 3 号 p. 197-
- 2 号 p. 99-
- 1 号 p. 1-
36 巻, 6 号
選択された号の論文の13件中1～13を表示しています
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大野 稔原稿種別: 本文
1967 年 36 巻 6 号 p. 487-507
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスIn order to investigate the significance of homopantothenic acid (HOPA) for the pathophysiology of mentally retarded children, HOPA was given orally to 60 children with various types of mental deficiency for 12 months, and clinical and electroencephalographic effects by the double-blind method were studied. The results are briefly summarized as follows : 1) The frequency analysis of waking occipital EEG was done by means of an automatic frequency analyzer. Administration of HOPA often depressed the energy percentage of slow waves (especially of δ-waves) and frequently aroused the energy percentage of α-waves. 2) HOPA raised the frequency of epileptic discharge on EEG. 3) Psychologically, HOPA often caused the increment of vocabulary and emotional excitement in mentally retarded children. 4) In the administration of HOPA over a long period of time, I.Q.was found to have increased over 10 % in 33.3 % of the children.抄録全体を表示PDF形式でダウンロード (3993K) -
勝又 増幸, 辻本 桂子, 木村 敬子, 木谷 敏子, 井上 ひろ子, 榊原 栄一原稿種別: 本文
1967 年 36 巻 6 号 p. 508-520
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスConcentration of pyruvic decarboxylase preparation from bakers' yeast unusually affected the enzymic activity. Therefore, theoretical analysis was attempted on the interrelation between the concentration of the enzyme preparation and enzymic activity. Following four situations of the reactions were postulated for the analysis : (1) The enzyme preparation contains inhibitor which reversibly combines with active enzyme. (2) The preparation contains activator which reversibly combines with enzyme. (3) The preparation is consisted of two enzymes which catalyze serial two reactions. (4) The preparation is consisted of two enzymes, and the substrote-enzyme complex serves the second enzymic reaction as a substrate. The interrelations are expressed by quadratic equations. The situations of (1), (2) and (4) are represented by hyperbolas or by parabolas, and that of (3) by two straight lines. The interrelations appeared in pyruvic decarboxylase preparations were explained by combination of the situations (1) and (3), indicating plural components, dissociable inhibitor and independent two enzymes. The possibility of the plural components in pyruvic decarboxylase preparation from bakers' yeast was also indicated by DEAE-cellulose column chromatography and by gel filtration with Sephadex G-100.抄録全体を表示PDF形式でダウンロード (1218K) -
中井 芳治, 大石 誠子, 清水 祥一, 福井 三郎原稿種別: 本文
1967 年 36 巻 6 号 p. 521-525
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスThe coenzyme forms of vitamin B_6 N-oxide, pyridoxal phosphate N-oxide and pyridoxamine phosphate N-oxide, were obtained as follows : Pyridoxal N-oxide was phosphorylated with P_2O_5 and H_3PO_4 after its aldehyde group was protected by p-toluidine. After hydrolysis of its poly-esters, the resulted Schiff base monophosphate dissolved in 2NNaOH and p-toluidine was removed with ether. The solution was neutralized and poured onto a column of Dowex 50X8 in its H^+ form. The yellow fractions (absorption maximum : 390mμ) were collected and concentrated to a minimum solution, from which pyridoxal phosphate N-oxide was crystallized. Pyridoxamine phosphate N-oxide was easily prepared from pyridoxamine N-oxide as follows : After phosphorylation and hydrolysis of poly-esters, the reaction mixture was concentrated to a minimum syrup which was mixed with absolute ethanol. The white precipitate was collected and dissolved in pyridine・water and the crystal of pyridoxamine phosphate N-oxide was obtained by adding acetone.抄録全体を表示PDF形式でダウンロード (507K) -
川崎 近太郎, 飯尾 利弘原稿種別: 本文
1967 年 36 巻 6 号 p. 526-529
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスMicrobiological assay of new modified thiamine compounds of 3 thiazionthiamine derivatives (I-a, b, c, ) and pseudothiamine (II) was studied ; only pseudo-acetothiamine-O-acetate (II) was proved to have about 1/1,000 the potency of thiamine on both the assays using L.fermenti and Kl.apiculata. The compound (II) is gradually hydrolysed to thiamine in the broth, therefore the microbiological activity of (II) was caused by the thiamine formed, and not by (II) itself. Phenylthiazinothiamine (Ia) was shown to be inhibitory to the growth of Kl.apiculata but not to be inhibitory to L.fermenti. Thiamine is extremely absorbable to Kloeckera cells but it was poorly absorbed on cells when (Ia) was present. Competitive inhibition of thiamine uptake on Kloeckera cells was demonstrated by (Ia), but not by (Ib).抄録全体を表示PDF形式でダウンロード (428K) -
川崎 近太郎, 篠田 純男, 小野 照代原稿種別: 本文
1967 年 36 巻 6 号 p. 530-534
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスThiamine, when preincubated with yeast cells in glucose solution, was almost completely absorbed on cells. The thiamine on cells could not be destroyed on the incubation with crude thiaminase from B.thiaminolyticus or Corbicula, although it was easily released from the cells on the digestion with cell-wall-lytic enzyme from Streptomyces albidoflaous. The stability of protoplasts prepared from thiamine-accumulated Kl.apiculata cells was decreased inversely proportional to thiamine contents. The highly accumulated thiamine on yeast cells was partly released by osmotic shock or during the process of fermentation, corresponding to the decrease of viable cells. The thiamine was completely released from sedimentation fractions of cells, after the treatment with Braun cell-desintegrator. The thiamine accumulated on yeast cells, was found as free thiamine located in the concentrated status between cell-walls and cytoplasmic membranes.抄録全体を表示PDF形式でダウンロード (599K) -
川崎 近太郎, 篠田 純男, 小野 照代原稿種別: 本文
1967 年 36 巻 6 号 p. 535-540
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスKloeckera apiculata, when incubated with thiamine in 2 % glucose solution for 1 hour, absorbed thiamine to the amount of one-tenth the cell weight but protoplasts prepared from Klosckera cells absorbed thiamine incompletely to the amount far less than that of intact cells. Thiamine diphosphate (TDP) was absorbed by the cells as free thiamine to the same extent as thiamine used but TDP was poorly absorbed by protoplasts. TDP, when incubated with intact cells in glucose and molybdate (M/10), was not converted to free thiamine and no absorption of thiamine by cells was demonstrated. S-Carbethoxythiamine (CET) and thiamine propyl disulfide (TPD) stimulated the growth of the yeast more strongly than thiamine but the both were absorbed on cells to the less extent than thiamine. Kl.apiculata, however, when innoculated in the broth containing 2μg of thiamine, CET or TPD, showed better growth and higher content of TDP in cells after incubation with CET or TPD than incubation with thiamine. CET, when incubated with higher quantity of the yeast cells, penetrated into cells by simple diffusion and was easily washed out by isotonic solution. Thiamine was accumulated on the cells completely and could not be washed out by isotonic solution. Penetration of CET into cytoplasm and conversion to TDP might be correlated with higher content of TDP in cells on growth tests.抄録全体を表示PDF形式でダウンロード (736K) -
川崎 近太郎, 篠田 純男原稿種別: 本文
1967 年 36 巻 6 号 p. 541-543
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスKloeckera apiculata cells repeatedly centrifuged at 1,500 rpm, were incubated in the broth at higher concentration of cell-counts for 2 or 4 hours and then they wer diluted with the broth containing thiamine or its derivatives ; synchronized growth of cells was observed on incubation for 2-6 hours. When thiamine, thiamine propyldisulfide (TPD) or S-carbethoxy-thiamine (CET) was added to the broth (0.125 μg equivalent), TPD stimulated the growth more quickly than thiamine at the first stage of growth, whereas CET and thiamine showed parallel synchronized growth, until at the last stage of logarithmic growth CET stimulated one more synchronized growth than thiamine. These differences of synchronized growth by TPD and CET will be originated from their different speed of conversion to thiamine.抄録全体を表示PDF形式でダウンロード (384K) -
糸川 嘉則, 井上 喜久子原稿種別: 本文
1967 年 36 巻 6 号 p. 544-547
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスA new method of the separation and determination of thiamine and its phosphoric esters (TMP and TDP) is described. By carboxylic acid ion exchanger (Amberlite IRC-50), thiamine and TMP were selectively taken up from the mixture of thiamine, TMP, and TDP, on the other hand TDP was not adsorbed by the resin but passed down completely. Thiamine and TMP can be removed from the resin with 0.05N HCl. Thiamine and TMP were adsorbed to activated zeolite (Permutit) and thiamine was selectively eluted with 25% KCl in 0.1N HCl, but TMP was not. Finally TMP, which was adsorbed to the zeolite, was eluted with 2N HCl completely. Each of these fractions (thiamine, TMP and TDP) is available to determination by thiochrome method.抄録全体を表示PDF形式でダウンロード (434K) -
中山 良夫, 藤井 建男, 甲和 良夫原稿種別: 本文
1967 年 36 巻 6 号 p. 548-556
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスSafety evaluation of pyridoxal-DL-homocysteine (PAL-HCySH) was made by acute-, subacute-, chronic-toxicity and teratological tests. In the acute toxicity test of mice and rats, LD_<50>s of PAL-HCySH were very high levels in any routes. In the oral subacute toxicity test of PAL-HCySH to male and female rats, the results obtained indicated very weak toxicity of the drug. In the chronic toxicity test, PAL-HCySH was administered to male rats orally, in doses of 125,250,500 and 1000mg/kg daily for seven months. With the exception of reduction of body weight gain in the rats given 1000mg/kg of PAL-HCySH, any toxic effects of the drug were not observed in the lower dosed groups. In the teratological test, PAL-HCySH was given orally to pregnant mice and rats every day from the 7th to the 14th day of gestation in doses of 5 and 1000mg/kg for mice and 5 and 500mg/kg for rats. No influence of PAL-HCySH was observed on fetuses obtained from the mother animals.抄録全体を表示PDF形式でダウンロード (973K) -
陳 震東原稿種別: 本文
1967 年 36 巻 6 号 p. 557-563
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスUptake of thiamine-^3H(B_1-^3H) and O-benzoylthiamine disulfide-^3H(BTDS-^3H) in eyes of mice with an oral administration has been studied by microautoradiography. The most marked uptake of B_1-^3H and BTDS-^3H was demonstrated in the retina and optic nerve. In the iris and ciliary body a moderate uptake was seen, while the cornea showed slight uptake of B_1-^3H and BTDS-^3H. In the retina a large amount of grains was observed in the layer of rods and cones, and then in ganglionic layer and layer of nerve fibers. In the iris and ciliary body distribution of the grains was most markedly observed in muscle fibers and then in the lining epithelium. Grains in the cornea was found in the basal layer. Uptake of BTDS-^3H was more marked than B_1-^3H in each portion of the eyes.抄録全体を表示PDF形式でダウンロード (1821K) -
小林 正原稿種別: 本文
1967 年 36 巻 6 号 p. 564-566
発行日: 1967/12/25
公開日: 2018/03/30
ジャーナル オープンアクセスIt was reported in the previous paper that a new compound "Bisteroid" was produced by the reaction of vitamin D with SbCl_3 reagent containing acetyl chloride and that the assumed structure contained a furan ring. However, the author found in recent investigations that the assumed structure had to be corrected in the following : (1) The "Bisteroid" reported in previous paper is a mixture of two componennts (crystalline A and B). (2) The crystalline A is a compound having the molecular formula C_<54>H_<84> and the crystalline B is a monoacetate having the molecular formula C_<56>H_<88>O_2. Both dimers originate from the condensation of 2 moles of vitamin D.抄録全体を表示PDF形式でダウンロード (339K) -
角田 美穂原稿種別: 本文
1967 年 36 巻 6 号 p. 567-581
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスThe comparison of three thiamine derivatives, thiamine hydrochloride (B_1), thiamine tetrahydrofurfuryldisulfide (TTFD) and thiamine diphosphate (TDP), was made using Vagus amine test in guinea pig under urethane anesthesia. The rapid injection of these thiamine derivatives induced ventricular arrhythmia, except in case of large dose of TTFD. These arrhythmias were based on catecholamine, released mainly from the reserpine depletable store and the guanoxan inhibitable, but still the direct action of thiamine existed. The degree of bradycardia suppression during vagal stimulation after rapid injection was stronger than the non-injected, according to the dosage increase of B_1 and TTFD. No difference was observed between B_1 and TTFD in the mean value of antiarrhythmic potency, but the percentage of effectiveness was larger and standard error of TTFD was smaller than those of B_1. Clinically, 50 mg of TTFD suppressed at least 45% of ventricular arrhythmia, and this action was more apparent in the patients with shorter history of arrythmia.抄録全体を表示PDF形式でダウンロード (1709K) -
内海 勇, 河野 啓一, 斉藤 一文字原稿種別: 本文
1967 年 36 巻 6 号 p. 582-586
発行日: 1967/12/25
公開日: 2018/02/10
ジャーナル オープンアクセスTDS, BTDS, BuTDS, iBuTDS and thiamine were orally administrated to man, rabbit and rat, and the intestinal absorption was statistically compared by urinary thiamine excretion or blood thiamine level. The urinary excretion for 24 or 48 hrs was not so much differed among these derivatives in rabbit, however, profound differences were observed in man. BTDS, having aromatic acyl groups, was shown to have the higher absorbability than that of BuTDS and iBuTDS, having aliphatic acyl groups. Thiamine and TDS, of fundamental structure, were only poorly absorbed giving the urinary excretion of one tenth or twentieth of acyl-TDS. In rat the similar tendency was also observed as in the case of man. The structure-activity correlation and species difference in the vitamin absorption were shortly discussed.抄録全体を表示PDF形式でダウンロード (528K)
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