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原稿種別: 目次
1962 年 25 巻 1 号 p.
Toc1-
発行日: 1962/01/25
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八木 國夫
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1962 年 25 巻 1 号 p.
1-12
発行日: 1962/01/25
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高田
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1962 年 25 巻 1 号 p.
12-
発行日: 1962/01/25
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高田
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1962 年 25 巻 1 号 p.
12-
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宮地 一馬, 安部 宗顕, 上野 裕己, 渡辺 正剛, 馬場 賢吾, 鹿上 昭三
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1962 年 25 巻 1 号 p.
13-19
発行日: 1962/01/25
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In gut mucosa of the rat and the mouse, succinic dehydrogenase and xanthine oxidase, especially the former, were strongly affected by riboflavin deficiency, while no influence was observed on DPNH-diaphorase actvity. On the basis of these results it is assumed that DPNH-diaphorase might be related to division of the epithelial cells of gut mucosa, since the enzymatic activity was not decreased by riboflavin restriction on gut mucosa where formation and decomposition of the enzyme would be accompanied with those of the epithelial cells. We further postulated that some symptoms of ariboflavinosis might be related to decrease of succinic dehydrogenase activity.
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高田
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1962 年 25 巻 1 号 p.
19-
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宮地 一馬, 馬場 賢吾, 安部 宗顕
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1962 年 25 巻 1 号 p.
20-22
発行日: 1962/01/25
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In a previous paper, we demonstrated that DPNH-diaphorase, contrary to succinic dehydrogenase, was little affected by riboflavin deficiency in gut mucosa where the epithelial cells are regenerated remarkably. The fact suggested that similar phenomena might be observed in the skin, known for its remarkable regenerarion as well as mucosa. Histochemical studies employing neotetrazolium salt showed, as expected, that succic indehydrogenase was extremely influenced, while DPNH-diaphorase was not, by riboflavin deflciency in the skin of the rat and the mouse.
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宮地 一馬, 上野 裕己, 渡辺 正剛, 馬場 賢吾, 石津 汪, 森園 義久, 安部 宗顕, 鹿上 昭三
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1962 年 25 巻 1 号 p.
22-30
発行日: 1962/01/25
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It has been reported that liver succinic dehydrogenase decreased in activity after a partial hapatectomy. However, there has been no report concerning the effect of partial hapatectomy on succinic dehydrogenase activity of the liver under the condition of riboflavin deficiency. Studies on this problem might give us some information on the relation of the enzyme to liver cell regeneration, because the decreased activity of the enzyme by riboflavin restriction might recover in the course of regeneration if the enzyme has any relation to it. The concentration of flavin and the activity of succinic dehydrogenase in the liver was decreased by the deficiency, but tended to recover as the liver regenerated. The high activity of DPNH-diaphorase in the liver was not so much decreased even after partial hapatectomy. These results may indicate that succinic dehydrogenase seems to be related to the regeneration, especially to cell differentiation.
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宮地 一馬, 馬場 賢吾, 上野 裕己, 渡辺 正剛, 森園 義久, 石津 汪, 鹿上 昭三
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1962 年 25 巻 1 号 p.
30-34
発行日: 1962/01/25
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As described in previous papers, DPNH-diaphorase seems to be concerned in cell division, whereas succinic dehydrogenase in cell differentiation. In this study, an attempt was made on proving the fact using Torulopsis utilis. Succinic dehydrogenase activity was very low at the period of rapid cell division when the organism was immature. However, a remarkable increase was observed at maturity. On the other hand DPNH-diaphorase activity was already high even at the period of rapid cell division, and furthermore the high activity was remained after the period. From these data we could prove the fact that DPNH-diaphorase is related to cell division and succinic dehydrogenase to cell differentiation.
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高田
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1962 年 25 巻 1 号 p.
34-
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宮地 一馬, 安部 宗顕
原稿種別: 本文
1962 年 25 巻 1 号 p.
35-40
発行日: 1962/01/25
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It has been reported that succinic dehydrogenase activity in cancer cells was low, whereas DPNH-diaphorase activity was high. However, little is known about the relation of the enzymatic activities to mitosis in cancer cells. We have carried out experiments to find out any relation of these enzymes to mitois using histochemical techniques to demonstrate activities of the two flavin enzymes, and a phase contrast microscopical technique to observe the nuclear states. Succinic dehydrogenase activity in the cancer cells was too low to prove the relation of the activity to the state of mitosis. About ten percent of the total cells was found to have DPNH-diaphorase activity. The most cells with DPNH-diaphorase activity were in state of mitosis, while cells having no activity were in resting state. From these results, we concluded that DPNH-diaphorase has some relation to cell mitosis.
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高田
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1962 年 25 巻 1 号 p.
40-
発行日: 1962/01/25
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高田
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1962 年 25 巻 1 号 p.
40-
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加藤 忠克, 清水 祥一
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1962 年 25 巻 1 号 p.
41-45
発行日: 1962/01/25
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Present paper deals with investigation on separation and determination of non-cyano type cobamides in fermentation products. An aqueous extract from feed supplement was used as a sample. Paper electrophoresis was conducted in 0.5N acetic acid and complete separation was attained for two hours. Each of separated components was eluted with potassium cyanide solution and then microbiological activity of each eluate was assayed with Ochromonas malhamensis. Results obtained showed that the aqueous extracts of feed supplement contain at least three Ochromonas malhamensis active cobamides, cyano-form (45%), hydroxoform (32%) and an unidentified form (23%). This unidentified cobamide is supposed to be protein-bound form.
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高田
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1962 年 25 巻 1 号 p.
45-
発行日: 1962/01/25
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高田
原稿種別: 本文
1962 年 25 巻 1 号 p.
45-
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加藤 忠克, 清水 祥一
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1962 年 25 巻 1 号 p.
46-49
発行日: 1962/01/25
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The present study was undertaken in order to obtain exact information about activity of hydroxocobalamin for Ochromonas malhamensis. When autoclaved together with Ford's assay medium in which cyanide was absent, hydroxocobalamin gave only approximately 20% of the response of cyanocobalamin. However, when autoclaving was conducted in the presence of ascorbate, difference was not observed in activities between hydroxocobalamin and cyanocobalamin. Also, the loss of activity in case of hydroxocobalamin could be avoided by employing the aseptic addition procedure. It is concluded from these results that hydroxocobalamin is as effective as cyanocobalamin on Ochromonas malhamensis.
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高田
原稿種別: 本文
1962 年 25 巻 1 号 p.
49-
発行日: 1962/01/25
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高田
原稿種別: 本文
1962 年 25 巻 1 号 p.
49-
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勝又 増幸, 辻本 桂子, 阪本 正子, 榊原 栄一
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1962 年 25 巻 1 号 p.
50-56
発行日: 1962/01/25
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The particulate fraction R_<12>, which contains mainly respiratory granule, and supernatant fraction S_<12> from B. aneurinolyticus were investigated on thiaminase activity and some enzymatic activities concerning in the role of thiaminase in this bacterial cell. Respiratory activity of R_<12> was observed only with succinate and α-ketoglutarate as substrates, and the addition of S_<12> was needed for utilization of glutamate or pyrrolidone carboxylate. Respiratory activity of R_<12> were controlled by ATP. S_<12> had no respiratory activity, however it showed the remarkable dehydrogenase activity, while R_<12> and S_<12> inter-depended on glutamate metabolism. The effect of S_<12> on aerobic glutamate metabolism of R_<12> was partially replaced by hydroxymethylpyrimidine, one of the products by thiaminase II action. Thiaminase II activity was predominant in S_<12> compared with R_<12>, however some enzymatic activity remained in R_<12>. This remained activity of R_<12> did not removed by washing with water. Glutamate-alanine transaminase activity was observed in S_<12> when it was measured by the estimation of alanine, but the reverse reaction was not observed. This phenomenon may suggest that the produced glutamate is converted into ninhydrin-negative substance, and the decomposition of glutamate was accelerated by hydroxymethylpyrimidine.
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高田
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1962 年 25 巻 1 号 p.
56-
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勝又 増幸, 辻本 桂子, 阪本 正子, 榊原 栄一
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1962 年 25 巻 1 号 p.
57-60
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On this paper, the spectrophotometric behaviors of the two fractions, S_<12> and R_<12>, which were separated from the cells of B. aneurinolyticus by the procedures as already described, were studied in the oxidoreduction states with several substrates, and moreover the fluorecent spectra of both deproteinized filtrates and those of denatured protein by urea were measured for the detection of flavin and other substances, and then the self-compensated functions of these fractions were investigated. In S_<12> (the supernatant fraction of centrifugation of 12,000 rpm) there were pyridinoprotein, flavoprotein containing FAD, a cytochrome-like substance which characterized by. the peak of 420mμ in difference spectra (α and β bands of this substance are trace) and an unidentified substance having a specific absorption at 315mμ in the difference spectra and the dehydrogenase activity for several kinds of substrates, R_<12> (the residual fraction of centrifugation of 12,000rpm) contained pyridinoprotein which took part in the metabolism of α-ketoglutarate and cytochromes which are detected by the peaks of difference spectra at 420,530 and 560mμ. Flavins were not detected in R_<12> as in S_<12>, so that these materials may exist in bound type. And also this fraction R_<12> showed the characteristic difference spectra with limited substances such as succinate, α-ketoglutarate and aspartate.
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川崎 近太郎, 富田 勲, 大良 勇
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1962 年 25 巻 1 号 p.
61-63
発行日: 1962/01/25
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By benzoylation of thiamine-disulfide (I), at least two products were obtained : Tetrabenzoate of (I), mp. 97-100℃ by the excess amount of benzoyl chloride and dibenzoate of (I), mp. 146-147℃ by the limited use of benzoyl chloride both in a pyridine or alkaline solution of (I), were isolated. Tetrabenzoate is sparingly soluble in acidic water and its structure is designated as (II) from its analytical value and infrared-spectrum. Beside dibenzoate (benzoylthiamine-disulfide) (III), a crystalline mixture of an unsharp mp. of 117-138℃ was obtained, when the reaction was carried out under ice-cooling. It contains two products, as its paper partition chromatogram indicated two spots ; Rf 0.57 and 0.74,of which 0.74 coincides with benzoylthiamine-disulfide.
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内海 勇, 原田 清, 平尾 宏子, 小寺 啓司
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1962 年 25 巻 1 号 p.
64-68
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Investigation was made on the physical and chemical properties of O-benzoylthiamine disulfide (BTDS), C_<38>H_<42>O_6N_8S_2. The crystal is colorless, odorless and nonhygroscopic. Studies on ultraviolet absorption of the aqueous solution was made at various pHs and showed that the absorption maxima are shifted with change of pH and the isobestic points are at 239mμ and 274mμ. The structure of BTDS was proved by the infrared spectral evidence. BTDS is sparingly soluble in water, ether and benzene, but soluble in acids and chloroform. Rf value is 0.85±0.03,when a developing solvent consisted of butanol, acetic acid and water is used. It was shown that BTDS is stable in a concentration of 2mg/ml not only in acidic enviroments, but in weak alkaline solutions. The powder of BTDS is very stable even when mixed with alkaline salts.
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高田
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1962 年 25 巻 1 号 p.
68-
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内海 勇, 原田 清, 小林 宏司, 河野 啓一, 安田 晃三, 近藤 陽子, 平尾 宏子
原稿種別: 本文
1962 年 25 巻 1 号 p.
69-74
発行日: 1962/01/25
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In the course of studies on thiamine disulfide we found that a new derivative, O-benzoyl thiamine disulfide (BTDS) is easily absorbed into organisms. BTDS exerts thiamine activity approximately equivalent to that of thiamine hydrochloride in a thiamine requring organism, rice bird and rat. BTDS is readily absorbed in the human body as well as in rabbit and rat, by oral administration and consequently the blood thiamine levels, the thiamine contents of organs and blood cocarboxylase activity rise remarkably and the risen levels last for a long time.
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内海 勇, 原田 清, 近藤 陽子, 平尾 宏子
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1962 年 25 巻 1 号 p.
74-78
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A fluorometric determination procedure of O-benzoylthiamine disulfide (BTDS) was established based on the results of fundamental investigations on the optimum condition for reduction of BTDS with sodium thiosulfate or cysteine. BTDS (1-100μg) was reduced quautitatively with 40mg of sodium thiosulfate at pH 4.0-5.0,60℃ for 20 minutes and with 5mg of cysteine hydrochloride at pH 8.4-8.8,37℃ for 60 minutes. Sodium thiosulfate reduced BTDS to O-benzoylthiamine (OBT) and a mixture of OBT and thiamine was produced with cysteine. The products were determined by the thiochrome method, since OBT was also converted to thiochrome with cyanogen bromide and alkali. When BTDS, thamine and esterified thiamine were contained together in blood, the total thiamine was satisfactorily determined as usual by the thiochrome method after hemolysis and diastase hydrolysis.
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小林 宏司, 安田 晃三, 甲和 良夫
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1962 年 25 巻 1 号 p.
79-83
発行日: 1962/01/25
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Pharmacological studies were made on O-benzoylthiamine disulfide (BTDS). BTDS produced moderate falls of blood pressure and scarecely influenced on respiration in rabbits and depressed the myocardial activity of isolated hearts of frogs. BTDS had almost no effects on the circulation of the perfused rabbits auricular vessels. Actions on the isolated intestines were decreased in the peristalisis and fall of the tension and no marked actions were observed on the movemement of uteri in rabbits. Acute toxicity in mice was much lower than that of thiamine hydrochloride, and examination of chronic toxicity did not reveal special hematologic and pathological changes. The experimental results mentioned above demonstrate that the pharmacological actions of BTDS are no less than those of thiamine hydrochloride and in toxicity BTDS has an advantage over thiamine hydrochloride.
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千畑 一郎, 山田 茂樹, 伊藤 博, 古津 節子, 内海 勇
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1962 年 25 巻 1 号 p.
83-85
発行日: 1962/01/25
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Studies were made on decomposition of thiamine disulfide and its derivatives by crude thiaminase of Bacillus thiaminolyticus and B. aneurinolyticus. Thiamine disulfide cetylsulfate, benzoylthiamine disulfide and acetylthiamine disulfide were found to be hardly destroyed even under the same conditions as thiamine hydrochloride was readily decomposed.
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千畑 一郎, 山田 茂樹, 伊藤 博, 古津 節子, 小野 正之, 内海 勇
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1962 年 25 巻 1 号 p.
85-91
発行日: 1962/01/25
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Investigation on the activities of thiamine disulfide (TDS) and its derivatives on the growth of Lactobacillus fermenti 36 and L. viridescens S38A was carried out under varied conditions. The activities of TDS and its cetylsulfate (TDS-CS) on these microorganisms in a medium omitting cysteine and cystine were lower than that of thiamine hydrochloride. Under the same conditions, O-propiorylthiamine disulfide and O-acetyl-, O-furoyl-and O-benzoylthiamine disulfide showed only a slight activity. However, the activities of TDS and TDS-CS were higher than that of thiamine hydrochloride when autoclaved with the basal medium containing cysteine and cystine. The other compounds showed 10-80 per cent activity of thiamine hydrochloride under the same condition.
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内海 勇, 原田 清, 平尾 宏子, 小寺 啓司
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1962 年 25 巻 1 号 p.
92-93
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内海 勇, 原田 清, 近藤 陽子, 平尾 宏子
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1962 年 25 巻 1 号 p.
93-94
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村田 希久
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1962 年 25 巻 1 号 p.
94-95
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舘 勇, 千田 光子, 千田 貢
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1962 年 25 巻 1 号 p.
95-96
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川崎 近太郎, 大良 勇, 小野 義子
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1962 年 25 巻 1 号 p.
96-97
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角尾 滋
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1962 年 25 巻 1 号 p.
97-98
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甲和 良夫, 小林 宏司, 安田 晃三, 内海 勇
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1962 年 25 巻 1 号 p.
98-99
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林 繰
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1962 年 25 巻 1 号 p.
99-100
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村田 希久
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1962 年 25 巻 1 号 p.
100-101
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千畑 一郎, 山田 茂樹, 伊藤 博, 古津 節子, 内海 勇
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1962 年 25 巻 1 号 p.
101-
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川崎 近太郎, 平岡 栄一, 山田 智佐江, 島本 隆光
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1962 年 25 巻 1 号 p.
101-103
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千畑 一郎, 山田 茂樹, 伊藤 博, 古津 節子, 小野 正之, 内海 勇
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1962 年 25 巻 1 号 p.
103-104
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林 良二, 中山 英男
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1962 年 25 巻 1 号 p.
104-106
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能勢 善嗣, 徳田 良弘, 上田 一恵
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1962 年 25 巻 1 号 p.
106-107
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福井 三郎, 前田 嘉道
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1962 年 25 巻 1 号 p.
107-110
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浜本 英次
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1962 年 25 巻 1 号 p.
110-111
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内海 勇, 原田 清, 小林 宏司, 河野 啓一, 安田 晃三, 近藤 陽子, 平尾 宏子
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1962 年 25 巻 1 号 p.
111-112
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川崎 近太郎, 富田 勲, 紀氏 健雄
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1962 年 25 巻 1 号 p.
112-115
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佐権 佳一
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1962 年 25 巻 1 号 p.
115-116
発行日: 1962/01/25
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荒木 正哉, 陳 震東, 梅村 泰一
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1962 年 25 巻 1 号 p.
116-117
発行日: 1962/01/25
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