巻号一覧
73 巻, 2 号
選択された号の論文の11件中1～11を表示しています
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三浦 洌原稿種別: 本文
1999 年 73 巻 2 号 p. 73-82
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーFlavocoenzymes are extremely versatile in that they show broad potentiality in catalysis, as manifested in the wide range of the reactions catalyzed by flavoenzymes. In order for each of the flavoenzymes to express its specific function, the versatility of the coenzyme should be elegantly controlled. This control mechanism lies in the structure-reaction linkage. We have recently solved the high-resolution three dimensional structure of D-amino acid oxidase by X-ray crystallography. On the basis of the tertiary structure of the enzyme-substrate analog complex, the enzyme-substrate complex structure was modeled by means of molecular mechanics simulation. The simulated structure was not compatible with the hitherto recognized reaction mechanisms which require an amino acid residue acting as a catalytic base to abstract the subtrate α-proton. We have introduced two new mechanisms after analyzing the structure-reaction linkage based on the substrate-binding mode and the requirements that the reaction mechanism should suffice. One of them is called the "electronproton-electron transfer mechanism" and is characterized by two one-electron transfer processes intervened by a proton transfer, while the other distinguishes itself in ionic intermediates and is thus called the "ionic mechanism." In both of the mechanisms flavin N(5) rather than an amino acid residue acts as a base for abstracting the substrate α-proton and flavin C(4_a) is the site of electron acceptance from the substrate.抄録全体を表示PDF形式でダウンロード (995K) -
岡山 和代, 福島 正子, 熊澤 幸子, 鈴江 緑衣郎原稿種別: 本文
1999 年 73 巻 2 号 p. 83-87
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーAluminium(Al) accumulates on nervous tissue and bone, and causes unfavorable syndromes on nephropathy patients. It is well known that some organic acids accelerate aluminium absorption. So we, at first, induced nephropathy of guinea pig by giving HgCl_2, and examined the effects of ascorbic acid(AsA) on aluminium absorption and excretion. Guinea pigs were divided into 5 groups. They were low AsA+A1 group, low AsA group, high AsA+Al group, high AsA group, and control group. Except for the control group, kidneys were damaged by intaking of HgC1_2. After 7 weeks, they were sacrificed and Al contents in organs were measured by atomic absorption spectrophotometer. On bone, Al contents were extremely high in AsA administered groups. But in the control group, A1 content showed normal value.抄録全体を表示PDF形式でダウンロード (549K) -
平岡 真実, 柴田 圭子, 安田 和人原稿種別: 本文
1999 年 73 巻 2 号 p. 89-94
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーAs an initial step to decide reference intervals of serum total vitamin B_6 and blood total nicotinic acid level, blood vitamin B_6 and nicotinic acid concentrations were determined in a group of healthy female students, 21-22 years old, who were confirmed with vitamin B_6 and nicotinic acid intake above their Recommended Dietary Allowances(RDA_s) by dietary survey. Serum vitamin B_6 and blood nicotinic acid were determined by isocratic postcolumn HPLC and reverse phase HPLC, respectively. Vitamin intakes were assessed from dietary record for consecutive 3 days. The mean of daily vitamin B_6 intake was 1.35±0.53mg/day and 13.0% of subjects showed lower than 0.016 on the ratio of vitamin B_6 (mg) / protein (g) intake. A significant correlation was found between intakes of vitamin B_6 and protein. Reference interval of serum total vitamin B_6 level which was obtained from the subjects with their intake above their RDA was 4 〜17 (9.1±2.7) ng/ml. The mean intake of the daily nicotinic acid equivalent was 24.0±5.9mg/day which was the amount of nicotinic acid and nicotinic acid converted from tryptophan. There was no subject who showed nicotinic acid intake lower than RDA (6.6mg/1,000kcal energy). Reference interval of blood total nicotinic acid level was 285〜710 (478.2±97.5)μg/dl from the subjects with nicotinic acid intake above their RDA.抄録全体を表示PDF形式でダウンロード (618K) -
村田 晃原稿種別: 本文
1999 年 73 巻 2 号 p. 95-98
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (497K) -
大塚 惠, 荒川 信彦原稿種別: 本文
1999 年 73 巻 2 号 p. 99-101
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (364K) -
堀尾 文彦原稿種別: 本文
1999 年 73 巻 2 号 p. 103-105
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (400K) -
吉岡 満城原稿種別: 本文
1999 年 73 巻 2 号 p. 107-
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (134K) -
重岡 成原稿種別: 本文
1999 年 73 巻 2 号 p. 109-114
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (734K) -
錦見 盛光原稿種別: 本文
1999 年 73 巻 2 号 p. 115-120
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (636K) -
岡 達三原稿種別: 本文
1999 年 73 巻 2 号 p. 121-122
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (348K) -
柘植 治人原稿種別: 本文
1999 年 73 巻 2 号 p. 122-124
発行日: 1999/02/25
公開日: 2017/12/26
ジャーナル フリーPDF形式でダウンロード (338K)
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