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奥村 健吾, 早川 昭
原稿種別: 本文
1961 年 23 巻 5 号 p.
333-336
発行日: 1961/08/25
公開日: 2017/12/22
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More than 2 mg of thiamine are adsorbed on one gram of potato starch and do not be eluted by water but by 5% aqueous solution of sodium chloride. The thiamine adsorbed on potato starch is rather stable without decomposition on standing at 38℃ for two months.
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奥村 健吾, 桜井 芳人
原稿種別: 本文
1961 年 23 巻 5 号 p.
337-339
発行日: 1961/08/25
公開日: 2017/12/22
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The colorimetric determination of thiamine tetrahydrofurfuryl disulfide (TTFD) was presented in this paper. TTFD was converted to thiamine with sodium thiosulfate. The resulting thiamine was directly estimated by the diazo method with p-aminoacetophenone, as the remaining thiosulfate did not disturb this color reaction.
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奥村 健吾
原稿種別: 本文
1961 年 23 巻 5 号 p.
339-344
発行日: 1961/08/25
公開日: 2017/12/22
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It was found that thiamine gave N-methyl thiamine in the addition of methyl iodide at 70℃ or methyl bromide at room temperature.
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東 秀雄, 山川 健重, 衣巻 豊輔, 杉井 麒三郎, 岩崎 喜久子
原稿種別: 本文
1961 年 23 巻 5 号 p.
344-356
発行日: 1961/08/25
公開日: 2017/12/22
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Methods previously proposed for the vitamin D determination are not available for separating vitamin D from impurities contained in fishes and fish products. However, a series of experiments the authors carried out, demonstrated that a combined use of clay chromatography and gradient alumina chromatography was highly efficient for the purpose. On the basis of studies on the nature of Japanese acid clay to be used for the chromatography, suitable conditions and methods for activation of the clay were secured. Satisfactory results were obtained in the experiments performed by the use of sample oils prepared from various parts of fishes, dried fishes and fish solubles.
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岡田 幸蔵
原稿種別: 本文
1961 年 23 巻 5 号 p.
356-360
発行日: 1961/08/25
公開日: 2017/12/22
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The destruction of dihydrothiamine (DHB_1) in animal bodies on its oral or intraperitoneal administration, was already observed by the author. The author found a thiamineforming factor in homogenates of various animal organs. On incubating DHB_1 with homogenates of heart, liver, spleen and kidney of rats or guinea pigs, transformation of DHB_1 into thiamine which was highest e.g. 9-12% of DHB_1 by kidney homogenates, was observed. The maximal conversion into thiamine by rat liver homogenate was 6% of DHB_1 at the optimum of pH and temperature (4.5 and 37℃) in 6 hours, while decomposition of DHB_1 was quick ; less than 10% of DHB_1 was found unchanged in 3 hours. The active substance capable to oxidize DHB_1 into thiamine was effective only at the presence of oxygen or air, and it lost most of its activity by boiling, dialysis or addition of SH-attacking agents such as potassium cyanide, sodium azide, or iodoacetate. Some of its activity was kept after precipitation by ammonium sulfate or acetone. Although this thiamine-forming factor seemed to be enzymatic, it was effective only in acid medium (pH 4.5) with a poor yield of thiamine. It will be most probable that DHB_1 is quickly decomposed or excreted partly unchanged into urins before its meager transformation into thiamine.
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川崎 近太郎, 山田 智佐江
原稿種別: 本文
1961 年 23 巻 5 号 p.
360-363
発行日: 1961/08/25
公開日: 2017/12/22
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DHB_1 (Dihydrothiamine) was oxidized almost quantitatively into thiamine, when its buffered acetic solution (pH 4) was kept at 40℃ for 10 minutes with the 50mg% solution of 2,6-dichlorophenolindophenol (I). Thus formed thiamine was adsorbed on a zeolite-column and was easily estimated by thiochrome method after its elution. Nearly quantitative reduction of (I) was also observed and the yield of thiamine from pseudo-DHB_1 was 90〜100% to the theoretical value, when 20-fold molecular equivalents of (I) were given to DHB_1. According to this procedure proposed by the authors, oxidation into thiamine was completed within 5 minutes, yielding the better results than the FeCl_3-oxidation method.
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松尾
原稿種別: 本文
1961 年 23 巻 5 号 p.
363-
発行日: 1961/08/25
公開日: 2017/12/22
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福井
原稿種別: 本文
1961 年 23 巻 5 号 p.
363-
発行日: 1961/08/25
公開日: 2017/12/22
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川崎 近太郎, 山田 智佐江
原稿種別: 本文
1961 年 23 巻 5 号 p.
364-367
発行日: 1961/08/25
公開日: 2017/12/22
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Dihydrothiamine (pseudo-DHB_1) was oxidized quantitatively into thiamine by 2,6-dichlorophenolindophenol, while n-DHB_1 was oxidized partly yielding varied amounts of thiamine according to solvents used for dissolving DHB_1. The yield of thiamine from n-DHB_1 was 58-64% to the theoretical value when n-DHB_1 was dissolved in dilute alcohol or water but it was much higher (e.g. 87%) when n-DHB_1 in absolute alcohol. If n-DHB_1 was first dissolved in HCl and then made alkaline by addition of NaHCO_3 or NaOH, the yield of thiamine was as high as that from pseudo-DHB_1 which was nearly quantitative. It was suggested, therefore, that n-DHB_1 had been converted into the pseudo-isomer in this prosedure. n-DHB_1 was unstable in its aqueous or dilute alcoholic solution resulting in hydrolysis into 5-hydroxy-3-mercapto-2-pentanone which was demonstrated by SH-detection by bis-(p-nitrophenyl)-disulfide, but n-DHB_1 in absolute alcohol showed a higher yield of thiamine indicating scarce hydrolysis. Comparing to the n-isomer, pseudo-DHB_1 was stable in aqueous dilute alcoholic or alkaline soluton and practically no presence of SH-radical by bis-(p-nitrophenyl)-disulfide in these solutions was indicated.
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高田
原稿種別: 本文
1961 年 23 巻 5 号 p.
367-
発行日: 1961/08/25
公開日: 2017/12/22
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小川 俊太郎, 小林 正
原稿種別: 本文
1961 年 23 巻 5 号 p.
368-371
発行日: 1961/08/25
公開日: 2017/12/22
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In 1960,F.J. Mulder of Philips-Duphar visited Japan, and introduced the vitamin A determination using saponification, benzene extraction, and determination of the extinction at 310,325,and 334 mμ on the isopropyl alcohol solution of unsaponifiable matter. We have tried out the whole oil method, USP method, and Mulder's method on USP vitamin A reference standard solution. When setting the results of the whole oil method as 100,USP method gave 99.2 and Mulder's method as 102.9. Similar results were obtained when various vitamin A preparations were tested. The reason of high value by Mulder's method is due to concentration of benzene solution during the determination. This high value (2〜3%) may be neglected in view of the permissible deviation in vitamin A determination, but Mulder's method gives more accurate value when multiplied factor 0.98.
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小川 俊太郎, 小林 正
原稿種別: 本文
1961 年 23 巻 5 号 p.
372-376
発行日: 1961/08/25
公開日: 2017/12/22
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The determination of vitamin D by USP XVI method has been studied. This method involves saponification, extraction, column partition chromatography, Fuller's earth adsorption chromatography, and correction colorimetrically by color inhibitor. Vitamin A can be separated from vitamin D by the partition chromatography, but vitamin A deconpositions can not be separated. This interfered substances can not be also separated throughly by the Fuller's earth chrometography. The correction factor of color inhibitor on the interfered substances are 0.26〜0.87 and not in accordance with 0.67 by USP.
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伊藤 うめの
原稿種別: 本文
1961 年 23 巻 5 号 p.
377-383
発行日: 1961/08/25
公開日: 2017/12/22
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Five new types (27 strains) of Lactobacillus pastorianus were isolated from draught beer et al., and they were temporarily named Bacillus B1,B2,B3,B4 and B5. Bacillus B1 is similar to L. malefermentans which was isolated by Walker et al. at Manchester from sour beer, since L. malefermentans is unable to proliferate in unhopped brewery wort. However, they are distinguished principally by the facts that the former ferments only fructose, maltose and glucose, and can not ferment inulin, while the latter can not ferment fructose, and ferments vigoroulsy inulin. Bacillus B1 is similar to L. heterohiochii since it requires essentially mevalonic acid. However, they are distinguished by the facts that the former can ferment maltose, and resists to hop antiseptic, and its growth is stimulated by CO_2. It is concluded, therefore, that Bacillus B1 represents a new type of Lactobacillus pastorianus hitherto undescribed. The characteristics : A non-motile, Gram-positive, catalase-negative rod. Optimum temperature for growth is 30℃, optimum pH value for growth is 4.8.
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松尾
原稿種別: 本文
1961 年 23 巻 5 号 p.
383-
発行日: 1961/08/25
公開日: 2017/12/22
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福井
原稿種別: 本文
1961 年 23 巻 5 号 p.
383-
発行日: 1961/08/25
公開日: 2017/12/22
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伊藤 うめの
原稿種別: 本文
1961 年 23 巻 5 号 p.
384-389
発行日: 1961/08/25
公開日: 2017/12/22
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Lactbacillus heterohiochii could not grow in fermented wort containing 10 mg/l isohumulone even after growing in fermented wort containing 5 mg/l isohumulone. L. heterohiochii could not induce the maltose-fermenting enzyme. However, Bacillus B1 could ferment maltose more vigorously than glucose. When Bacillus B1 was incubated in the modified medium III for Hiochi-bacteria used by Kitahara et al., but without mevalonic acid, in CO_2-atmosphere, it fermented maltose and glucose producing mevalonic acid-like substance with assimilation of CO_2. However, L. heterohiochii did not ferment. Therefore, it is concluded that Bacillus B1 is a new species other than L. heterohiochii, since the former has three specific characters described above. It was noticed that there was the enzyme system involving mevalonic acid-like substance by which fructose can not be fermented, and that there was the enzyme system involving mevalonic acid by which fructose can be fermented.
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伊藤 うめの
原稿種別: 本文
1961 年 23 巻 5 号 p.
390-395
発行日: 1961/08/25
公開日: 2017/12/22
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The most of characteristics of Bacillus B2 are very similar to those of Bacillus B1 investigated previously. However, Bacillus B1 and B2 are distinguished principally by the facts that the latter does not require mevalonic acid, but requires essentially ascorbic acid and biotin (under existence of Tween 80), and produces ethanol from fructose, and is sensitive to dehydroacetic acid. It is suggested that Bacillus B2 produces mevalonic acid through assimilation of CO_2,because Bacillus B2 does not require mevalonic acid and requires essentially biotin, and that Bacillus B1 and B2 differs in phosphorous metabolism conjugated with glycolysis, since Bacillus B2 produces ethanol from fructose, and is sensitive to dehydroacetic acid. It is considered that the requirement toward mevalonic acid for Lactobacillus can not be applied as the taxonomical factor toward Lactobacillus. Bacillus B1 has lost the requirement toward mevalonic acid after treatment with antifungal substances. Bacillus B2 has lost the requirement toward peptides after treatment with antifungal substances. It is proposed that Bacillus B2 is a variety of Bacillus B1.
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伊藤 うめの
原稿種別: 本文
1961 年 23 巻 5 号 p.
396-401
発行日: 1961/08/25
公開日: 2017/12/22
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The most of the characteristics of Bacillus B3 are very similar to those of Bacillus B1 investigated previously, but Bacillus B3 is different from Bacillus B1 and Bacillus B2 reported previously, because, the former requires promotively mevalonic acid, and ferments fructose hardly in CO_2-atmosphere, but can in N_2-atmosphere in a medium without mevalonic acid. Bacillus B3 seemes to produce mevalonic acid-like substance more simply than mevalonic acid in CO_2-atmosphere, because it ferments fructose hardly in CO_2-atmosphere. The catalase reaction on Bacillus B4 is positive, therefore, the Bacillus B4 is taxonomically peculiar. The most of the characteristics of Bacillus B4,except catalase reaction are very similar to those of Bacillus B1,B2 and B3,but the former does not require mevalonic acid and requires promotively peptides. The peptides required for the growth of Bacillus B3 and B4 are sensitive to heat, but those by Bacillus B1 and B2 resist to heat. It is proposed that both Bacillus B3 and B4 are the new varieties of Bacillus B1.
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高田
原稿種別: 本文
1961 年 23 巻 5 号 p.
401-
発行日: 1961/08/25
公開日: 2017/12/22
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[記載なし]
原稿種別: 本文
1961 年 23 巻 5 号 p.
401-
発行日: 1961/08/25
公開日: 2017/12/22
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桂
原稿種別: 本文
1961 年 23 巻 5 号 p.
401-
発行日: 1961/08/25
公開日: 2017/12/22
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伊藤 うめの
原稿種別: 本文
1961 年 23 巻 5 号 p.
402-407
発行日: 1961/08/25
公開日: 2017/12/22
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Bacillus B5 is a non-motile, Gram-positive, catalase-negative rod. Optimum temperature for growth is 30℃, optimum pH is 5.0-6.0,minimum pH is 8.6,but can grow at above pH 7.8. It resists to hop antiseptic, its growth is promoted by CO_2. All ten strains require essentially uracil and purines, but not require mevalonic acid and peptides. All of the strains ferment arabinose, xylose, glucose, galactose, maltose and sucrose, but can not ferment fructose, and fermentability of glucose is considerably inferior to that of maltose. Bacillus B5 seems to ferment sugars through the enzyme system involving mevalonic acid-like substance, and to ferment maltose through pathway of phosphorilysis from the view point of fermentability of sugars. It is proposed that Bacillus B5 is a new variety of Lactobacillus pastorianus. Heterofermentative "Hiochi-Lactobacilli" donnot require uracil and purines, therefore, it is concluded that they are the different species on the origin.
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高田
原稿種別: 本文
1961 年 23 巻 5 号 p.
407-
発行日: 1961/08/25
公開日: 2017/12/22
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伊藤 うめの
原稿種別: 本文
1961 年 23 巻 5 号 p.
408-414
発行日: 1961/08/25
公開日: 2017/12/22
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Based on the four peculiar characteristics, i.e. (1) assimilation of CO_2,(2) resistance to hop antiseptic, (3) specific fermentability of maltose, and (4) requirement for uracil and purines, all the beer-spoiling Lactobacilli including L. malefermentans (found by Russel et al.), L. pastorianus (Van Laer), Bacilli L3 (Moore et al.) et al. and the authors Bacilli seemed to be collected to an independent taxonomical position in spite of the diversity of types of fermentable sugar. Therefore, after modifying Lactobacillus pastorianus, the species name for the beer-spoiling Lactobacillus, to Lactobacillus cerevisiae, and this new species was subdivided into the four types, i.e.α, β, γ and δ, since beer-spoiling Lactobacilli could be classified into four groups from the view point of fermentability of sugars. Accordingly, Bacillus B1,B2,B3,B4 and B5 were named respectively Lactobacillus cerevisiae δ (nov. sp.), L.c. δ var. I (nov. var.), L.c. δ var. II (nov. var.), L.c. δ var. catalasus (nov. var.) and L.c. β (nov. sp.). It is proposed that the four types of Lactobacillus cerevisiae are inserted in brevis-series in the Kitahara's new classification system.
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高田
原稿種別: 本文
1961 年 23 巻 5 号 p.
414-
発行日: 1961/08/25
公開日: 2017/12/22
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高田
原稿種別: 本文
1961 年 23 巻 5 号 p.
414-
発行日: 1961/08/25
公開日: 2017/12/22
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高田
原稿種別: 本文
1961 年 23 巻 5 号 p.
414-
発行日: 1961/08/25
公開日: 2017/12/22
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高田
原稿種別: 本文
1961 年 23 巻 5 号 p.
414-
発行日: 1961/08/25
公開日: 2017/12/22
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満田 久輝, 河合 文雄, 鈴木 譲
原稿種別: 本文
1961 年 23 巻 5 号 p.
415-
発行日: 1961/08/25
公開日: 2017/12/22
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満田 久輝, 河合 文雄, 鈴木 譲
原稿種別: 本文
1961 年 23 巻 5 号 p.
415-416
発行日: 1961/08/25
公開日: 2017/12/22
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満田 久輝, 河合 文雄, 中山 行穂
原稿種別: 本文
1961 年 23 巻 5 号 p.
416-
発行日: 1961/08/25
公開日: 2017/12/22
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塚田 陽二, 杉森 恒武, 今井 和民, 片桐 英郎
原稿種別: 本文
1961 年 23 巻 5 号 p.
416-
発行日: 1961/08/25
公開日: 2017/12/22
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堀田 一雄, 勝沼 信彦, 石黒 伊三雄
原稿種別: 本文
1961 年 23 巻 5 号 p.
416-
発行日: 1961/08/25
公開日: 2017/12/22
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宮地 一馬, 馬場 賢吾, 渡辺 正剛, 上野 裕巳, 石津 汪, 森園 義久
原稿種別: 本文
1961 年 23 巻 5 号 p.
416-417
発行日: 1961/08/25
公開日: 2017/12/22
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上原 喜八郎, 小橋 恭一
原稿種別: 本文
1961 年 23 巻 5 号 p.
417-418
発行日: 1961/08/25
公開日: 2017/12/22
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今永 一
原稿種別: 本文
1961 年 23 巻 5 号 p.
418-
発行日: 1961/08/25
公開日: 2017/12/22
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牧野 堅
原稿種別: 本文
1961 年 23 巻 5 号 p.
418-419
発行日: 1961/08/25
公開日: 2017/12/22
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林 髞
原稿種別: 本文
1961 年 23 巻 5 号 p.
419-
発行日: 1961/08/25
公開日: 2017/12/22
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福井 三郎, 酒沢 千嘉弘, 上原 悌次郎
原稿種別: 本文
1961 年 23 巻 5 号 p.
419-
発行日: 1961/08/25
公開日: 2017/12/22
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佐橋 佳一, 妹尾 信雄
原稿種別: 本文
1961 年 23 巻 5 号 p.
419-420
発行日: 1961/08/25
公開日: 2017/12/22
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奥田 邦雄
原稿種別: 本文
1961 年 23 巻 5 号 p.
420-
発行日: 1961/08/25
公開日: 2017/12/22
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能勢 善嗣
原稿種別: 本文
1961 年 23 巻 5 号 p.
420-421
発行日: 1961/08/25
公開日: 2017/12/22
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鈴木 友二
原稿種別: 本文
1961 年 23 巻 5 号 p.
421-
発行日: 1961/08/25
公開日: 2017/12/22
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川崎 近太郎, 岡田 幸蔵
原稿種別: 本文
1961 年 23 巻 5 号 p.
421-422
発行日: 1961/08/25
公開日: 2017/12/22
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川崎 近太郎, 平岡 栄一, 肥田 政彦
原稿種別: 本文
1961 年 23 巻 5 号 p.
422-
発行日: 1961/08/25
公開日: 2017/12/22
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川崎 近太郎, 山田 智佐江
原稿種別: 本文
1961 年 23 巻 5 号 p.
422-
発行日: 1961/08/25
公開日: 2017/12/22
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川崎 近太郎, 山田 智佐江, 小野 義子
原稿種別: 本文
1961 年 23 巻 5 号 p.
422-423
発行日: 1961/08/25
公開日: 2017/12/22
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角尾 滋
原稿種別: 本文
1961 年 23 巻 5 号 p.
423-
発行日: 1961/08/25
公開日: 2017/12/22
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宮地 一馬, 秋山 俊夫, 有山 英世
原稿種別: 本文
1961 年 23 巻 5 号 p.
423-424
発行日: 1961/08/25
公開日: 2017/12/22
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中村 恒男, 能勢 修, 山崎 厳
原稿種別: 本文
1961 年 23 巻 5 号 p.
424-
発行日: 1961/08/25
公開日: 2017/12/22
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