In order to establish pyridoxine as a precursor of the pyrimidine moiety of thiamin in Saccharomyces cerevisiae, labeled pyridoxines, [^<15>N]-, [2'-^<13>C]- and [6-^<13>C]pyridoxines were chemically synthesized with alanine, formic acid and ethyl maleate via oxazole derivative. [^<15>N]-, [2'-^<13>C]- and [6-^<13>C]Pyridoxines were labeled with [^<15>N]-, [3-^<13>C]alanine and [^<13>C]formic acid, respectively. [5'-^2H_2]Pyridoxine was synthesized by the reduction of isopropylidene pyridoxic acid methyl ester with LiAID_4 followed by hydrolysis.
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