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川崎 近太郎, 飯尾 利弘, 近藤 雅臣
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1969 年 40 巻 2 号 p.
59-63
発行日: 1969/08/25
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Thiamine was easily adsorbed by cells of Sacch. cerevisiae in Reader's medium, when cells had been harvested at the exponential phase, but it was poorly adsorbed by cells at the later stationary phase. When the cells at the stationary phase had been incubated for growth in a fresh medium, the cells harvested, were demonstrated to be restored to adsorb thiamine strongly. The extraordinary accumulation of thiamine in yeast cells was shown to be correlated to their status of harvesting rate of growth, number of budding cells and higher contents of nucleic acids in cells, as shown in Fig. 3 and Table 3. It was suggested that the thiamine accumulation in yeast cells depended upon the active physiological state of the cells prior to the cell division.
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川崎 近太郎, 飯尾 利弘, 近藤 雅臣
原稿種別: 本文
1969 年 40 巻 2 号 p.
64-66
発行日: 1969/08/25
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Saccharomyces cerevisiae can synthesize thiamine from its pyrimidine and thiazole moieties but the rate of synthesis varied with cells, harvested in different media and reaction media for synthesis. The highest yield on synthesis was demonstrated by cells harvested in Atkin's medium and by the biosynthesis in Reader's medium. There was found higher yield on synthesis by cells harvested at the exponential phase than those at the stationary phase. The thiamine synthesized, was always found in cells, not in medium and about 1/4 of the thiamine was shown as its phosphorylated forms.
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原稿種別: 本文
1969 年 40 巻 2 号 p.
67-71
発行日: 1969/08/25
公開日: 2018/02/22
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原稿種別: 本文
1969 年 40 巻 2 号 p.
71-
発行日: 1969/08/25
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原稿種別: 本文
1969 年 40 巻 2 号 p.
71-
発行日: 1969/08/25
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川崎 近太郎, 飯尾 利弘, 近藤 雅臣
原稿種別: 本文
1969 年 40 巻 2 号 p.
71-
発行日: 1969/08/25
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Biosynthesis of thiamine from its pyrimidine and thiazole moieties by Sacch. cerevisiae harvested in the medium containing thiamine or its pyrimidine and thiazole moieties, was shown to be more retarded than that by cells harvested in the medium without thiamine or with either pyrimidine or thiazole moiety. When the cells harvested in the medium without thiamine, had been incubated with thiamine for 1 or 3 hours before the reaction of biosynthesis, no retardation for biosynthesis was demonstrated. Biosynthesis of thiamine by crude enzyme extracted from cells harvested in the thiamine-containing medium was also proved to be more retarded and less phosphorylated than that from cells in thiamine-free medium. Under the retarded biosynthesis of thiamine by yeast cells, it was shown that uptake of the pyrimidine moiety by cells from the medium was also repressed.
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山本 良子, 八木 国夫
原稿種別: 本文
1969 年 40 巻 2 号 p.
72-75
発行日: 1969/08/25
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Riboflavin tetrabutyrate was dissolved in fresh soybean oil to 50 μg/ml, which was irradiated with Mazda daylight lamp. After irradiation for 4 hours, the absorbance at 445 mμ was decreased to 41% of the original absorbance, but the decrease was recovered up to 56% by mere storage in the dark for 48 hours. However, when the solution was irradiated for 10 hours, this recovery remained only 26%. These results suggest that riboflavin tetrabutyrate dissolved in the oil can be partly reduced by irradiation and reversed through slow oxidation. The irreversible reaction could be attributed to the decomposition of the isoalloxazine nucleus of riboflavin tetrabutyrate due probably to its reaction with lipoperoxide produced in the oil upon irradiation.
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真田 浩, 田口 博国
原稿種別: 本文
1969 年 40 巻 2 号 p.
83-
発行日: 1969/08/25
公開日: 2018/02/22
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Twenty-five inpatients of a psychiatric clinic showed high incidence of the sign of folic acid deficiency. Low serum folate levels (L. casei assay) was revealed in 64% of the patients and macrocytosis (MCV over 95μ) in 64%. Hypersegmented polymorphs were increased in 24%. Mean diameter of the nucleus of the oral epithelial cells were a little greater than normal. On the other hand, serum iron levels, formiminoglutamic acid excretion after histidine loading and myelogram were normal. After the oral administration of 5mg of folic acid for 30 days, macrocytosis and hypersegmented polymorphs disappeared. The growth of L. casei or L. leichmannii was not suppressed in vitro by addition of various anticonvulsants in the medium.
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上田 文雄, 牧野 輝勇, 豊平 暢子
原稿種別: 本文
1969 年 40 巻 2 号 p.
84-90
発行日: 1969/08/25
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A method for the separation and determination of vitamin A and α-tocopherol in mixtures by gel filtration using the column of Sephadex LH-20 was examined. Hydrolysis of the samples was carried out in ethanolic potassium hydroxide by addition of antioxidants. The unsaponifiable fraction was extracted with ether in usual method. The extracted vitamin A and α-tocopherol were fractionated on Sephadex LH-20 column as the gel bed and chloroform as the eluting solvent. After treatment of gel filtration vitamin A and α-tocopherol were determined using the spectrophotometrric assay for vitamin A and Emmerie・Engel assay for α-tocopherol. Separation of vitamin A and α-tocopherol was achieved successfully by this method and recoveries were satisfactory.
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原稿種別: 本文
1969 年 40 巻 2 号 p.
96-
発行日: 1969/08/25
公開日: 2018/02/22
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中山 英男, 松岡 洋子
原稿種別: 本文
1969 年 40 巻 2 号 p.
96-
発行日: 1969/08/25
公開日: 2018/02/22
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Cell-free extracts of a mutant strain of Escherichia coli, W2-3,which required either thiamine or succinate for aerobic growth, and of its parent strain, W, were compared for their ability to catalyze overall oxidation of 2-oxoglutarate. Both extracts catalyzed the reduction of NAD in the presence of 2-oxoglutarate, CoA, and thiamine diphosphate, though the former was less active than the latter. Subsequently, both extracts catalyzed the anaerobic evolution of carbon dioxide from 2-oxoglutarate in the presence of ferricyanide as electron acceptor with variable amounts of thiamine diphosphate. The rate of carbon doixide evolution was always higher in the extracts prepared from the wild type strain than those prepared from the mutant strain at the same concentration of thiamine diphosphate. These results suggest that the biochemical lesion which was altered genetically in the mutant strain was at a stage in the decarboxylation of 2-oxoglutarate and that the stage was not blocked completely but the enzymic properties might be changed and became to require thiamine diphosphate in excess.
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足立 透, 朝比奈 正人, 椎名 亨
原稿種別: 本文
1969 年 40 巻 2 号 p.
100-
発行日: 1969/08/25
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After intraperitoneal injection of nicotinic acid, FMN and riboflavin tetranicotinate (B_2-Nic) into rats, the amounts of free fatty acid, triglyceride, phospholipid and cholesterol in the serum were studied on time schedule. Free fatty acid was decreased in a short time after the injection of nicotinic acid and B_2-Nic, though the decrease by B_2-Nic seemed to be somewhat delayed, but it again increased to the higher value than the normal, 8 hours after the injection. On the contrary, it was increased in a short time after the injection of FMN. Triglyceride was markedly decreased by nicotinic acid and B_2-Nic, and considerably by FMN. Cholesterol and phospholipid was also decreased by all these compounds to some extent.
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小原 喜重郎, 小野 繁, 高橋 康文, 日下 純男
原稿種別: 本文
1969 年 40 巻 2 号 p.
103-
発行日: 1969/08/25
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The change in riboflavin metabolism of dog's salivary glands was investigated when autonomic blocking agents were administered subcutaneously. The contents of total riboflavin in the three salivary glands were found to increase at 30 minutes and to decrease than normal levels at 60 minutes after an administration of atropine. Total riboflavin content decreased in the stage of hypersecretion in parotid and submandibular gland by administration of pilocarpine, whereas it increased in the sublingual gland. Decrease of FAD and increases of FMN and free riboflavin recognized in the three salivary glands by administration of atropine. FAD fraction in the parotid increased characteristically at 30 minutes after administration of pilocarpine. From these results, the authors suggest that there is a close correlation between salivary secretion and riboflavin metabolism, furthermore, that the parotid and the submandibular glands are similar organs functionally, because of similar response was found in these organs for the administration of autonomic blocking agents.
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若生 宏, 畠山 富而, 鈴木 鐘美
原稿種別: 本文
1969 年 40 巻 2 号 p.
104-
発行日: 1969/08/25
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太幡 利一, 鈴木 マリナ, 大熊 啓汪, 小林 順子, 清水 泰幸
原稿種別: 本文
1969 年 40 巻 2 号 p.
104-105
発行日: 1969/08/25
公開日: 2018/02/22
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小林 正, 旗谷 充代, 藪田 昌子, 石川 幸子
原稿種別: 本文
1969 年 40 巻 2 号 p.
105-
発行日: 1969/08/25
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津郷 友吉, 山田 邦男, 菅野 長右エ門
原稿種別: 本文
1969 年 40 巻 2 号 p.
106-
発行日: 1969/08/25
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小原 正郎, 中野 浩, 河嶋 新一郎
原稿種別: 本文
1969 年 40 巻 2 号 p.
107-
発行日: 1969/08/25
公開日: 2018/02/22
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勝沼 信彦, 賀治 佐知子
原稿種別: 本文
1969 年 40 巻 2 号 p.
107-
発行日: 1969/08/25
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武田 克之
原稿種別: 本文
1969 年 40 巻 2 号 p.
107-108
発行日: 1969/08/25
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角尾 滋
原稿種別: 本文
1969 年 40 巻 2 号 p.
108-109
発行日: 1969/08/25
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清水 正夫, 鈴木 忠生, 亀田 欣也, 安孫子 雍史
原稿種別: 本文
1969 年 40 巻 2 号 p.
109-
発行日: 1969/08/25
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鈴木 忠生, 安孫子 雍史, 清水 正夫
原稿種別: 本文
1969 年 40 巻 2 号 p.
109-110
発行日: 1969/08/25
公開日: 2018/02/22
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宮川 正澄, 伊藤 元
原稿種別: 本文
1969 年 40 巻 2 号 p.
110-111
発行日: 1969/08/25
公開日: 2018/02/22
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佐橋 佳一, 前川 昭男, 古谷 博, 渡辺 泰猛
原稿種別: 本文
1969 年 40 巻 2 号 p.
111-
発行日: 1969/08/25
公開日: 2018/02/22
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奥田 邦雄, 八島 啓輔, 高松 政俊
原稿種別: 本文
1969 年 40 巻 2 号 p.
112-
発行日: 1969/08/25
公開日: 2018/02/22
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奥田 邦雄
原稿種別: 本文
1969 年 40 巻 2 号 p.
112-113
発行日: 1969/08/25
公開日: 2018/02/22
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福井 三郎, 清水 祥一, 山田 良平, 梅谷 友信
原稿種別: 本文
1969 年 40 巻 2 号 p.
113-
発行日: 1969/08/25
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林 良二, 中山 英男
原稿種別: 本文
1969 年 40 巻 2 号 p.
113-114
発行日: 1969/08/25
公開日: 2018/02/22
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村岡 三郎
原稿種別: 本文
1969 年 40 巻 2 号 p.
114-
発行日: 1969/08/25
公開日: 2018/02/22
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田村 善蔵
原稿種別: 本文
1969 年 40 巻 2 号 p.
114-115
発行日: 1969/08/25
公開日: 2018/02/22
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竹内 勝, 富岡 容子
原稿種別: 本文
1969 年 40 巻 2 号 p.
116-
発行日: 1969/08/25
公開日: 2018/02/22
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梶田 武俊, 千田 貢
原稿種別: 本文
1969 年 40 巻 2 号 p.
116-
発行日: 1969/08/25
公開日: 2018/02/22
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渡辺 至子, 萩沼 之孝, 桜井 芳人
原稿種別: 本文
1969 年 40 巻 2 号 p.
116-117
発行日: 1969/08/25
公開日: 2018/02/22
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宮川 正澄
原稿種別: 本文
1969 年 40 巻 2 号 p.
117-
発行日: 1969/08/25
公開日: 2018/02/22
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