The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Volume 23 , Issue 3
Showing 1-12 articles out of 12 articles from the selected issue
  • MAMORU ARAI, KIYOSHI HAMANO
    1970 Volume 23 Issue 3 Pages 107-112
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Isolation of azalomycins F3, F4 and F5 from azalomycin F-complex crystals was successfully conducted. F3 was less soluble in methanol than other components and was isolated by fractional crystallization of F-complex from 80-100% methanol in water. F4 and F5 were separated by P-cellulose column chromatography, from which F5 and F4 were eluted with 60 % aqueous methanol and 0.01 M ammonium acetate in 60 % aqueous methanol, respectively. Determination of physico-chemical as well as biological properties pointed to a very close resemblance of the structures of these components.
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  • I. CHARACTERISTICS OF THIOPEPTIN B
    NORIMASA MIYAIRI, TOSHIO MLYOSHI, HATSUO AOKI, MASANOBU KOHSAKA, HIROI ...
    1970 Volume 23 Issue 3 Pages 113-119
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The microbiological characteristics of Streptomyces strain No. 7906 include formation of white aerial mycelium, light brown growth and thick aerial hyphae forming tufts. Strain No. 7906 was compared with known species and identified as a new species and given the name Streptomyces tateyamensis. Streptomyces tateyamensis No. 7906 produces new sulfur-containing peptide antibiotics which are named thiopeptins. The major component, thiopeptin B, is obtained as a crystalline powder decomposing at 219-222°C, and exhibiting optical rotation [a]23D -80°(c 1, chloroform). Its elementary analysis suggested the empirical formula C72H90O22N18S6. The ultraviolet absorption maxima (shoulder) were found at 230-250, 295 and 305 mμ. Thiopeptin B showed strong antibacterial activity against Gram-positive bacteria and had no cross resistance with penicillins, aminoglycoside antibiotics, tetracyclines and macrolides. The administration of 500 mg/kg of this antibiotic into mice by intraperitoneal route did not result in any toxic symptom.
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  • GARY G. MARCONI, MIKLOS BODANSZKY
    1970 Volume 23 Issue 3 Pages 120-124
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The absolute configuration of stendomycidine (I), a constituent of the peptide antibiotic stendomycin, was determined. Optical rotatory dispersion (ORD) measurements showed the configuration at the α-carbon atom to be that of an L-amino acid. Degradation of stendomycidine with N-bromosuccinimide yielded 2-methylimino-3-methyl-hexahydropyrimidine-4-carboxylic acid (II), a compound with only one center of asymmetry. A comparison of the ORD spectrum of compound II with that of 2-imino-hexahydropyrimidine-4-carboxylic acid (III), prepared from authentic L-α, γ-diaminobutyric acid, showed that the second center of asymmetry also has the L-configuration. Hence, the L-erythro configuration was assigned to stendomycidine. Comparison of the nmr spectra of stendomycidine and capreomycidine added some additional support to the above assignment.
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  • RYUJI SHINOBU, NOBUO KANDA
    1970 Volume 23 Issue 3 Pages 125-130
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    A Streptomyces species, originally designated as No. 751, isolated from soil, was carefully studied from the standpoint of its morphological, physiological, and cultural characteristics. Because it produces soluble, deep purplish-red pigment in mixed culture with various microorganisms, but does not produce such pigment when cultivated independently, it is proposed as a new species and is designated Streptomyces propurpuratus nov. sp. SHINOBU et KANDA. The type strain is designated as OEU No. 751. It is not difficult to imagine that, in nature, a product of metabolism of a microorganism might be changed by the effect of a product of metabolism of another microorganism, whether the reactions are chemical or enzymatic. The authors believe that such phenomenaoccur not only with regard to pigment formation, but also with regard to production of antibiotics. An example of such a phenomenon is reported herein.
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  • SYNTHESIS AND CHEMICAL PROPERTIES OF CEFAZOLIN
    KAZUO KARIYONE, HIROKICHI HARADA, MASARU KURITA, TADAYOSHI TAKANO
    1970 Volume 23 Issue 3 Pages 131-136
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Cefazolin, 7-[l-(l H)-tetrazolylacetamido]-3-[2-(5-methyl-l, 3, 4-thiadiazolyl-thiomethyl]-Δ3-cephem-4-carboxylic acid, is a new semisynthetic antibiotic having a broad spectrum of antibacterial activity. A synthesis of cefazolin and its chemical properties are described.
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  • IN VITRO AND IN VIVO ANTIMICROBIAL ACTIVITY
    MINORU NISHIDA, TADAO MATSUBARA, TAKEO MURAKAWA, YASUHIRO MINE, YOSHIK ...
    1970 Volume 23 Issue 3 Pages 137-148
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Cefazolin is a new antibiotic derived from 7-aminocephalosporanic acid, having the structure of 7-[l-(lH)-tetrazolylacetamido]-3-[2-(5-methyl-l, 3, 4-thiadiazolyl)-thiomethyl]-Δ3-cephem-4-carboxylic cid. This substance is a broad-spectrum antibiotic, active in vitro against most of Gram-positive and Gram-negative species of bacteria except for Ps. aeruginosa, and is also active against penicillinase-producing strains of Staph. aureus. The activity of cefazolin against fresh isolates of E. coli and Kl. pneumoniae seems superior to those of other antibiotics used in this study. The in vitro activity of cefazolin is little influenced by size of inoculum, presence of rabbit serum, or kinds or properties of test medium. The activity of cefazolin is apparently bactericidal against both Gram-positive and Gram-negative bacteria at or above the MIC levels. In experiments on the development of resistance in vitro, the MICs of cefazolin against Staph. aureus strain 209P and E. coli strain NIHJ increase slowly in a stepwise over a period of 17 transfers. The rate of resistance development of cefazolin is comparable with that of cepholoridine or ampicillin. Cefazolin is relatively stable to enzymes from Staph. aureus and E. coli, which readily inactivate ampicillin and benzyl penicillin. Cefazolin is not remarkably degraded by the tissue homogenates of rat, although cephalothin is degraded quite rapidly. Cefazolin, in the single subcutaneous dose, shows excellent protecting activity against experimental infections in mice infected with both PC-sensitive and resistant strains of Staph. aureus, and is also fairly effective against infections in mice with Diplococcus pneumoniae, E. coli and Proteus mirabilis.
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  • AGNES A. BODANSZKY, MIKLOS BODANSZKY
    1970 Volume 23 Issue 3 Pages 149-154
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Acid hydrolysis of the peptide antibiotic amphomycinliberates a group of five basic amino acids. The major components of this mixture were isolated in pure form and identified as L, -threo-α, β-diaminobutyric acid and D-erythro-α, β-diaminobutyric acid. Both occur in the parent molecule. The basic amino acids present in minor amounts are also discussed.
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  • TAXONOMY, ISOLATION AND CHARACTERIZATION
    TAKASHI SHOMURA, NORIO EZAKI, TAKASHI TSURUOKA, TOMIZO NIWA, EIICHI AK ...
    1970 Volume 23 Issue 3 Pages 155-161
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    A new antibiotic SF-733 inhibiting both Gram-negative and Gram-positive bacteria was isolated from Streptomyces ribosidificus nov. sp., strain SF-733. Antibiotic SF-733 obtained as colorless needles has a molecular formula C17H34N4O10, and is concluded to be a new aminoglycosidic, water-soluble and basic antibiotic based on its physical and chemical properties.
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  • AKIHIRO MATSUMAE, EDWARD C. CANTINO
    1970 Volume 23 Issue 3 Pages 162-165
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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  • MASARU SHIMURA, TETSURO WATANABE, TAKAO OHASHI, TAKASHI SHOMURA, TARO ...
    1970 Volume 23 Issue 3 Pages 166-167
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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  • AKIKO KATO, KUNIO ANDO, GAKUZO TAMURA, KEI ARIMA
    1970 Volume 23 Issue 3 Pages 168-169
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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  • MASA HAMADA, Tomio Tadeuchi, SHINICHI KONDO, YOKO IKEDA, HIROSHI NAGAN ...
    1970 Volume 23 Issue 3 Pages 170-171
    Published: March 25, 1970
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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