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I. Taxonomy, Fermentation, Isolation and Biological Activities
MAKOTO UBUKATA, NORIO SHIRAISHI, KIMIE KOBINATA, TAKUJI KUDO, ISAMU YA ...
1995 Volume 48 Issue 4 Pages
289-292
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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Three novel 36-membered macrolide antibiotics, RS-22A, B and C produced by
Streptomyces violaceusniger have been isolated. These antibiotics were purified from an acetone extract of the mycelia followed by butanol extraction, centrifugal partition chromatography and HPLC. RS-22A, B, C showed antimicrobial activity against fungi and Gram-positive bacteria.
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II. Physico-chemical Properties and Structure Elucidation
MAKOTO UBUKATA, TETSU-ICHIRO MORITA, HIROYUKI OSADA
1995 Volume 48 Issue 4 Pages
293-299
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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RS-22A, B and C are novel 36-membered macrolide antibiotics produced by
Streptomyces violaceusniger. The structures of these antibiotics were unambiguously determined by ID and 2D NMR and tandem-mass analysis of the oxidative derivative of RS-22C.
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PASCALE HOEHN, ORESTE GHISALBA, THEOPHILE MOERKER, HEINRICH H. PETER
1995 Volume 48 Issue 4 Pages
300-305
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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3'-Demethoxy-3'-hydroxystaurosporine, 1 (CGP 58 546), a novel Staurosporine analogue, was isolated from a mutant of
Streptomyces longisporoflavus R19 blocked in the last step of the biosynthetic pathway. CGP 58 546 was less potent than Staurosporine, but it showed a more selective inhibition pattern against various subtypes of protein kinase C.
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DAVID J. FROST, KIM D. BRANDT, DAVID CUGIER, ROBERT GOLDMAN
1995 Volume 48 Issue 4 Pages
306-310
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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A whole-cell
C. albicans screen was designed to identify novel inhibitors interacting with the synthesis, assembly and regulation of the fungal cell wall.
C. albicans was grown in a paired broth assay in 96-well plates with natural product extracts or pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol. Growth was visually examined over a 7-day period and scored into different growth categories. Positives from the sorbitol rescue were then examined under the microscope for morphological alterations and grouped into several morphological classes. Sorbitol protection and cell morphology were indicators of novel antifungal agents from natural product extracts and pure compounds.
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ROBERT F. MIDDLETON, GRAHAM FOSTER, RICHARD J. P. CANNELL, PHILIP J. S ...
1995 Volume 48 Issue 4 Pages
311-316
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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Microorganisms were screened for the ability to modify the squalene synthase inhibitor squalestatin
1.
Biotransformation of
1 by two actinomycetes, SI5106 and SI5138, yielded three products hydroxylated on the 4, 6-dimethyl-oct-2-enoyl side chain either at the 6 position (
5) or 7 position (
4 two diastereoisomers), and lacking the acetyl ester from the C-1 side chain. Many strains were found to hydrolyse the 4, 6-dimethyl-oct-2-enoyl or acetyl esters to yield squalestatins
2 or
3.
The 3-methyl ester (
6) of
1 was obtained using
Fusarium sp. F13945. This fungus also produced a farnesoic acid derivative, possibly in response to inhibition of its squalene synthase by
1. The biotransformation products of
1 all retained potent squalene synthase inhibitory activity.
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HIROYUKI KUMAGAI, MICHIYO OSONO, MASATOMI IIJIMA, MIYUKI SAKASHITA, MA ...
1995 Volume 48 Issue 4 Pages
317-320
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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Action of cytogenin on macrophages and T cells was investigated. Phagocytosis of yeast and production of PMA-elicited superoxide anion by macrophages taken from mice given cytogenin po were augmented. Cytogenin enhanced productions of IL-1α by macrophages and IFNγ and GM-CSF by spleen cells although it did not enhanced production of TNFα by macrophages and IL-6 by macrophages and spleen cells. Macrophages stimulated with cytogenin caused to stimulate proliferation of purified T cells in Intercell cultures in which each cell population was cultured without contact. Results suggest that cytogenin primarily activates macrophages to produce monokines such as IL-1α and it causes to stimulate proliferation and differentiation of T cells resulting in production of lymphokines such as IFNγ and GM-CSF.
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HIROYUKI KUMAGAI, TORU MASUDA, MIYUKI SAKASHITA, MASAAKI ISHIZUKA, TOM ...
1995 Volume 48 Issue 4 Pages
321-325
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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Cytogenin recovered the reduced mitogenic response to Con A of spleen cells of tumor bearing mice
in vitro. The suppressive factor(s) was detected in adherent cell population in spleen cells. The reduced antitumor effector activity of spleen cells taken from tumor bearing mice was also augmented by the treatment with cytogenin
in vitro. The effect of cytogenin was neutralized by the treatment with anti-Mac 1 serum. Administration of cytogenin inhibited the production of nitric oxide by macrophages which is known as one of suppressor factors. Results indicate that one possible action of cytogenin exhibiting antitumor activity in tumor bearing mice may be due to modulation of Mac 1 positive cells.
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MASAHIRO TANAKA, KAZUO SHIN-YA, KAZUO FURIHATA, HARUO SETO
1995 Volume 48 Issue 4 Pages
326-328
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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MIN CHU, IMBI TRUUMEES, MAHESH PATEL, CHRISTINE BLOOD, PRADIP R. DAS, ...
1995 Volume 48 Issue 4 Pages
329-331
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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New HIV-1 Protease Inhibitors, Produced by Streptomyces sp. ATCC 55925
S. STEFANELLI, L. CAVALETTI, E. SARUBBI, E. RAGG, L. COLOMBO, E. SELVA
1995 Volume 48 Issue 4 Pages
332-334
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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MASAYUKI IGARASHI, CHIAKI SASAO, ATSUHITO YOSHIDA, HIROSHI NAGANAWA, M ...
1995 Volume 48 Issue 4 Pages
335-337
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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KHISAL A. ALVI, CHRISTOPHER D. REEVES, JOHN PETERSON, JOSEPH LEIN
1995 Volume 48 Issue 4 Pages
338-340
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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FRÉDÉRIC LEGENDRE, MARIE DOLORÈS MATURANO, GILLES ...
1995 Volume 48 Issue 4 Pages
341-343
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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MASAKO KATAGIRI, MASAHIKO HAYASHI, KEIICHI MATSUZAKI, HARUO TANAKA, SA ...
1995 Volume 48 Issue 4 Pages
344-346
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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KAOQIN LIU, ROBERT L. WHITE, JIAN-YONG HE, LEO C. VINING
1995 Volume 48 Issue 4 Pages
347-348
Published: April 25, 1995
Released on J-STAGE: April 19, 2006
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