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I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Biological Activity
YUYA YOSHIMOTO, TSUTOMU SAWA, NAOKO KINOSHITA, YOSHIKO HOMMA, MASA HAM ...
2000 Volume 53 Issue 6 Pages
569-574
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
JOURNAL
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A new compound, MK800-62F1, was isolated from a cultured broth of
Streptomyces diastatochromogenes MK800-62F1. It inhibited H
2O
2-induced apoptosis in human small cell lung carcinoma Ms-1 cells as well as in human T-cell leukemia Jurkat cells. In addition, MK800-62F1 also inhibited camptothecin-induced apoptosis in Jurkat cells, which was mediated by intracellular H
2O
2 generation. MK800-62F1 did not exhibit antioxidative activity
in vitro, suggesting that inhibition of apoptosis by MK800-62F1 was not due to the scavenging of H
2O
2, rather it was due to the modulation of the downstream event of H
2O
2 generation.
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II. Structure Elucidation
YUYA YOSHIMOTO, TSUTOMU SAWA, HIROSHI NAGANAWA, TAKESHI SUGAI, TOMIO T ...
2000 Volume 53 Issue 6 Pages
575-578
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
JOURNAL
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A new compound, MK800-62F1, was isolated from a cultured broth of
Streptomyces diastatochromogenes MK800-62F1. The structure was determined by NMR analysis and degradation experiments.
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I. Taxonomy, Fermentation, Isolation, Physico-chemical Properties and Structure Elucidation
NOBUAKI NARUSE, RENA KAGEYAMA-KAWASE, YASUHIRO FUNAHASHI, TOSHIAKI WAK ...
2000 Volume 53 Issue 6 Pages
579-590
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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A new family of capillary tube formation inhibitors, designated luminacins, has been discovered in the fermentation broth of a soil bacterium. The strain was identified as
Streptomyces sp. Mer-VD1207 from taxonomic studies. By means of a series of chromatographic procedures, fourteen structurally related components, luminacins A
1, A
2, B
1, B
2, C
1, C
2, D, E
1, E
2, E
3, F, G
1, G
2, and H, were isolated, and their structures were elucidated on the basis of spectroscopic analyses.
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II. Biological Activities
TOSHIAKI WAKABAYASHI, RENA KAGEYAMA-KAWASE, NOBUAKI NARUSE, YASUHIRO F ...
2000 Volume 53 Issue 6 Pages
591-596
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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Twelve of the fourteen isolated components of the luminacin family were assayed for activity to inhibit capillary tube formation
in vitro. Seven of them showed potent activity with IC
50 values of less than 0.1μg/ml in a rat aorta matrix culture model. Luminacin D, the strongest inhibitor, inhibited both endothelial cell proliferation and capillary tube formation. Morphological observation suggested that luminacin D inhibited the rearrangement of endothelial cells in the initial stage of tube formation. Luminacins and their derivatives are good candidates for application as angiogenesis inhibitors with a novel mechanism of action.
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I. Production, Isolation, Physico-chemical Properties and Biological Activities
TSUTOMU SATO, DAISUKE ISHIYAMA, REIKO HONDA, HISATO SENDA, HIROSHI KON ...
2000 Volume 53 Issue 6 Pages
597-602
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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A novel antifungal cyclic depsipeptide termed glomosporin, which has a fatty acyl side chain, was isolated from a barley solid culture of
Glomospora sp. The strain was isolated from fallen pine leaves collected in Fukusima Prefecture, Japan and identified as
Glomospora sp. BAUA2825.
Glomosporin was purified by butanol extraction followed by preparative HPLC. Glomosporin showed antimicrobial activity against fungi including clinically important
Aspergillus fumigatus.
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I. Taxonomy, Fermentation, and Biological Activities
SATOSHI OMURA, NORIKO ARAI, YUUICHI YAMAGUCHI, ROKURO MASUMA, YUZURU I ...
2000 Volume 53 Issue 6 Pages
603-608
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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A new chitinase inhibitor, named argifin, was isolated from the cultured broth of a fungal strain FTD-0668. The strain was identified as
Gliodadium sp. from morphological characteristics. The IC
50 value of argifin against
Lucilia cuprina chitinase was 3.7μM. Argifin arrested the moult of cockroach larvae upon injection into the ventral abdominal part.
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II. Isolation, Physico-chemical Properties, and Structure Elucidation
NORIKO ARAI, KAZURO SHIOMI, YUZURU IWAI, SATOSHI OMURA
2000 Volume 53 Issue 6 Pages
609-614
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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A new chitinase inhibitor, named argifin, was isolated from the cultured broth of a fungal strain
Gliodadium sp. FTD-0668. Argifin was purified from the cultured mycelium by the combination of cation exchange, anion exchange, adsorption, and gel filtration chromatographic methods. The structure of argifin was elucidated as cyclo(
Nω-(
N-methylcarbamoyl)-L-arginyl-
N-methyl-L-phcnylalany1-β-l-aspartyl-β-laspartyl-D-alanyl) by NMR experiments and other spectroscopic analyses.
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III. Structure Determination
SEIJI YOSHIMURA, BUNJI SATO, TAKAYOSHI KINOSHITA, SHIGEHIRO TAKASE, HI ...
2000 Volume 53 Issue 6 Pages
615-622
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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During the course of screening for novel cell cycle inhibitors, FRl 82877 was isolated from the fermentation broth of
Streptomyces sp. No.9885. During the NMR measurements, FRl 82877 decomposed so much that the structure elucidation of FRl 82877 itself was difficult. Then, combinations of chemical correlations and spectroscopic methods clarified that FRl 82877 possesses an unprecedented multi-ring system including the strained double bond, which was unexpectedly epoxidized by molecular oxygen. FRl 82877 showed broad antitumor activities
in vitro and promoted assemblies of tublins
in vitro as well as taxol. It is noteworthy that epoxidation of the distorted double bond resulted in significant decrease in antitumor activities.
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LIBRADA M. CAÑEDO, LUIS COSTA, LUIS M. CRIADO, JOSÉ L. F ...
2000 Volume 53 Issue 6 Pages
623-626
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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Isolation and Structure Elucidation
VENETA IVANOVA, VICTORIA GESHEVA, MARIANA KOLAROVA
2000 Volume 53 Issue 6 Pages
627-632
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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ERIKO TOYODA, MASAHARU HIRATSUKA, KYUICHI NEMOTO, MAKOTO YONEDA, CHIKA ...
2000 Volume 53 Issue 6 Pages
633-636
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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NAOKI MATSUMOTO, NAOKI AGATA, HIROSHI KUBOKI, HIRONOBU IINUMA, TSUTOMU ...
2000 Volume 53 Issue 6 Pages
637-639
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
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BIRENDRA N. PRAMANIK, PETIA A. SHIPKOVA, PETER L. BARTNER, LARRY HEIMA ...
2000 Volume 53 Issue 6 Pages
640-643
Published: June 25, 2000
Released on J-STAGE: September 19, 2008
JOURNAL
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