The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Volume 37 , Issue 12
Showing 1-35 articles out of 35 articles from the selected issue
  • JAMES P. KARWOWSKI, MARIANNA JACKSON, THOMAS A. BOBIK, JOSEPH F. PROKO ...
    1984 Volume 37 Issue 12 Pages 1513-1518
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    Spenolimycin, a new spectinomycin-type antibiotic, was discovered in the fermentation broth of a new actinomycete named Streptomyces gilvospiralis sp. nov. strain AB634D-177. Although a small amount of spectinomycin is coproduced by strain AB634D-177, the culture is different from other known spectinomycin-producing actinomycetes. Spenolimycin was obtained by conventional submerged culture in 14-liter fermentors with a peak antibiotic titer of 140 μg/ml.
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  • JAMES B. MCALPINE, GREGORY M. BRILL, STEPHEN G. SPANTON, SANDRA L. MUE ...
    1984 Volume 37 Issue 12 Pages 1519-1524
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    A new water-soluble, basic antibiotic has been isolated from the fermentation beers of Streptomyces gilvospiralis sp. nov. The structure of the antibiotic has been deduced from spectral studies and confirmed by chemical degradation to spectinomycin. This structure, 3'-O -methylspectinomycin-3', 4'-enol ether has led to the name spenolimycin.
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  • PRABHAVATHI B. FERNANDES, CHARLENE M. VOJTKO, ROBERT R. BOWER, JONINA ...
    1984 Volume 37 Issue 12 Pages 1525-1527
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    Spenolimycin is a new spectinomycin-type antibiotic isolated from Streptomyces gilvospiralis sp. nov. In vitro, it was active against a wide variety of aerobic Gram-positive and Gramnegative bacteria and Neisseria gonorrhoeae. It was two to four-fold more active against N. gonorrhoeae than spectinomycin. Spenolimycin was effective in the standard mouse protection test against Escherichia coli, Klebsiella pneumoniae and Streptococcus pneumoniae.
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  • HIDEO ONO, YUKIMASA NOZAKI, NOZOMI KATAYAMA, HISAYOSHI OKAZAKI
    1984 Volume 37 Issue 12 Pages 1528-1535
    Published: 1984
    Released: April 19, 2006
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    Three Gram-negative bacteria produce new cephem antibiotics, named cephabacins, with unique 3-side chains. Cephabacins include F group antibiotics with a 7-formylamino substituent and H group antibiotics without the substituent. The producing bacteria were taxonomically characterized and designated as Lysobacter lactamgenus sp. nov. YK-90 and Xanthomonas lactamgena sp. nov. YK-278 and YK-280.
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  • SETSUO HARADA, SHIGETOSHI TSUBOTANI, HIDEO ONO, HISAYOSHI OKAZAKI
    1984 Volume 37 Issue 12 Pages 1536-1545
    Published: 1984
    Released: April 19, 2006
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    Fifteen components of new antibiotics, cephabacins, were isolated from the culture filtrates of Lysobacter lactamgenus YK-90, Xanthomonas lactanigenaYK-280 and X. lactaingena YK-278. They were purified by column chromatography using cation-exchange resins, activated carbon, high porous resins and cation-exchange Sephadex and by preparative reverse-phase HPLC. The basic, water-soluble antibiotics were characterized as having a cephem skeleton and oligopeptide(s) as a side chain constituent from their spectroscopic analyses and amino acid analyses.
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  • SHIGETOSHI TSUBOTANI, TSUNEAKI HIDA, FUMIKO KASAHARA, YOSHIKAZU WADA, ...
    1984 Volume 37 Issue 12 Pages 1546-1554
    Published: 1984
    Released: April 19, 2006
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    The structures of 15 new cephem antibiotics, cephabacin F1-9 and H1-6, were determined by their spectroscopic analyses and decomposition studies. They are consisted of a cephalosporin nucleus and a di, tri or tetrapeptide including a new amino acid which is bound at the position 3 with an ester bond. The components, F1-9, showed unique biological activities by the presence of a formylamino group at the position 7.
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  • YUKIMASA NOZAKI, KENJI OKONOGI, NOZOMI KATAYAMA, HIDEO ONO, SETSUO HAR ...
    1984 Volume 37 Issue 12 Pages 1555-1565
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    Cephabacin F group antibiotics with a 7-formylamino substituent showed antibacterial activity against a wide variety of bacteria including β-lactamase-producing clinical isolates and anaerobic bacteria. Cephabacin H group antibiotics without the substituent showed more potent activity against Gram-positive bacteria than cephabacin F group antibiotics, but were not active against Gram-negative bacteria producing β-lactamases. Cephabacin F group antibiotics were highly resistant to hydrolysis by various types of β-lactamases and showed strong inhibitory activity against a cephalosporinase of Proteus vulgalis GN 4413 due to the 7-formylamino substituent. Mode of action of cephabacin F1 and H1 was examined using Escherichia coli and Bacillus subtilis as the test organisms. They showed strong lytic activity against these organisms and inhibited their peptidoglycan synthesis. Cephabacin F1 had the highest affinity for penicillin-binding protein (PBP) 1 in E. coli and PBP 4 in B. subtilis. Cephabacins showed a protective effect in experimentally infected mice.
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  • R. H. BUNGE, T. R. HURLEY, T. A. SMITKA, N. E. WILLMER, A. J. BRANKIEW ...
    1984 Volume 37 Issue 12 Pages 1566-1571
    Published: 1984
    Released: April 19, 2006
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    The isolation of two new antibiotics, PD 114, 759 and PD 115, 028, exhibiting in vivo antitumor activity at extremely low doses is described. The physico-chemical properties of these sulfur-containing compounds show that they represent a novel class of antitumor agents.
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  • SATOSHI OMURA, YOSHITAKE TANAKA, YOKO TAKAHASHI, IRI CHIA, MASAHARU IN ...
    1984 Volume 37 Issue 12 Pages 1572-1578
    Published: 1984
    Released: April 19, 2006
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    Irumamycin is a new 20-membered macrolide antibiotic isolated from a culture broth of a soil isolate which was named Streptomyces subflavus subsp. irumaensis AM-3603. It is active in vitro against some phytopathogenic fungi, but inactive against most aerobic and anaerobic bacteria and mycoplasmas. The potent in vitro activity and the results of preliminary pot tests indicated that the antibiotic is practicable as an agricultural antifungal agent.
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  • TOMIZO NIWA, TAKASHI TSURUOKA, HITOSHI GOI, YOSHIO KODAMA, JIRO ITOH, ...
    1984 Volume 37 Issue 12 Pages 1579-1586
    Published: 1984
    Released: April 19, 2006
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    A new aminosugar named nojirimycin B (1) has been isolated as its bisulfite adduct from the culture broth of Streptomyces lavendulae SF-425, together with nojirimycin. Microbiological oxidation of 1 with Gluconobacter suboxydans IAM 1829 gave a δ-lactam (2). The structures of 1and 2 were determined to be 5-amino-5-deoxy-D-mannopyranose and D-mannonic-δ-lactam, respectively, on the basis of 1H NMR spectroscopy and X-ray structural analysis. Both 1 and2 exhibited powerful inhibitory activity against rat epididymal α-mannosidase and apricot β-glucosidase.
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  • TAKASHI KAWAGUCHI, TAKASHI ASAHI, TOMOKI SATOH, TAKESHI UOZUMI, TERUHI ...
    1984 Volume 37 Issue 12 Pages 1587-1595
    Published: 1984
    Released: April 19, 2006
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    "Curing" treatment of a rifamycin -producing Nocardia sp. resulted in a mutant deficient in the synthesis of antibiotics. This deficiency was reversed in a medium containing yeast extract. The active substance, named B-factor, which induced rifamycin production in the mutant was purified from yeast extract, and its structure, 3'-(1-butylphosphoryl) adenosine, was determined by structural analysis and chemical synthesis. An extremely low concentration of B-factor (10 ng/ml) caused recovery of rifamycin B synthesis in the mutant and stimulated synthesis of the antibiotic in the parental strain.
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  • G. C. S. REDDY, R. S. SOOD, S. R. NADKARNI, J. REDEN, B. N. GANGULI, H ...
    1984 Volume 37 Issue 12 Pages 1596-1599
    Published: 1984
    Released: April 19, 2006
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    The absolute configuration of the ring system of the antibiotic G7063-2 has been established as being the same as that reported for terreic acid, based on circular dichroism data. During structure elucidation experiments, reaction with ethereal diazomethane gave an adduct whose structure is proposed.
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  • FRANCOISE PEYPOUX, MARIE-THÉRÈSE POMMIER, BHUPESH C. DAS ...
    1984 Volume 37 Issue 12 Pages 1600-1604
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    The complete structures of bacillomycin D and bacillomycin L were revised by FAB mass spectrometry and by Edman degradation of the derivatives resulting from the N-bromosuccinimide reaction. The homologous components of both series of antibiotics were separated by HPLC and the β-amino acids were identified by capillary gas chromatography.
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  • HISAMI KITAMURA-MATSUNAGA, YUKIO KIMURA, TOYOSHIGE ARAKI, NOBUO IZUMIY ...
    1984 Volume 37 Issue 12 Pages 1605-1610
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    Commercial colistin (polymyxin E)-complex was separated into two major components (colistins A and B) on a preparative scale by HPLC (alkyl bonded silica and aqueous-organic mobile phase containing 0.2 M NaCl-HCl buffer (pH 2.0)). Desalting of the colistins A and B fractions was completed by reversed-phase adsorption and elution using methyl alcohol. In these experiments, it was inadvertently found that prolonged elution with water gave two hydrophilic peptides, which were tentatively named colistins AH and BH, respectively. Further elution with methyl alcohol produced two lipophilic peptides which were named colistins AL and BL. Colistin BH showed higher potency than colistin AH, AL or BL, and it was also effective in vivo. The fatty acid and amino acid composition of colistin BH was identical with that of colistin BL, but colistin BA contained a relatively large amount of nonionic sodium which scarcely responded to the sodium ion-selective electrode of an ion meter; colistin BH had a slightly lower molar extinction coefficient than the colistin BL which contained negligible amount of nonionic sodium. Colistin AH also contained nonionic sodium. Potassium containing colistins could also be derived from colistin-complex. These compounds could not be formed merely by adding sodium chloride or potassium chloride to colistin-complex solution. To obtain the sodium-containing compounds, contact with some hydrophobic stationary phase, such as alkyl-bonded silica or styrene-divinylbenzene copolymer, was necessary. It is postulated that one of the antibacterial mechanisms of polymyxin is associated with its complex-forming action on monovalent cations after contact with the lipid layer of the bacterial outer membrane.
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  • MARIO BRUFANI, LUCIANO CELLAI, EGIDIO MARCHI, ANNALAURA SEGRE
    1984 Volume 37 Issue 12 Pages 1611-1622
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    Two series of new semisynthetic rifamycin SV derivatives have been prepared. One of them bears a quaternary ammonium salt at C3 (1-5). and the other a pyridoimidazo system condensed at C3 and C4 (6-11). While compounds 1-5 had poor antibacterial activity in vitro, compounds 6-11 were found to be highly active in vitro but poorly absorbed in vivo. They could thus have potential as agents in the therapy of intestinal infections. The synthesis and the 1H NMR structure determination of these new compounds are reported.
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  • MARIO BRUFANI, LUCIANO CELLAI, SILVIO CERRINI, WALTER FEDELI, EGIDIO M ...
    1984 Volume 37 Issue 12 Pages 1623-1627
    Published: 1984
    Released: April 19, 2006
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    This paper reports the determination of the X-ray molecular structure of 4-deoxy-3'-bromopyrido[1', 2'-1, 2]imidazo[5, 4-c]rifamycin S, carried out in order to unequivocally define the general structure of a new series of rifamycin SV derivatives, which are potent antibacterial agents, and are not absorbed at the gastroenteric level. They have been prepared by Alfa Farmaceutici, Bologna, by condensing 2-aminopyridine derivatives to 3-bromorifamycin S. The solid state X-ray study has confirmed the structure proposed on the basis of 1H NMR studies in solution. It has also shown that the newly formed pyridoimidazo system is in a mesomeric betaine form, the pyrido nitrogen being positively charged and the imidazo nitrogen being negatively charged.This feature is believed responsible for the pharmacokinetic behavior of these new drugs, one of which, denoted either as rifamycin L 105 or rifaximin, is actually under clinical trial as a topical intestinal disinfectant.
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  • SHUICHI SAKAMOTO, TSUTOMU TSUCHIYA, AKIHIRO TANAKA, SUMIO UMEZAWA, MAS ...
    1984 Volume 37 Issue 12 Pages 1628-1634
    Published: 1984
    Released: April 19, 2006
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    Derivatives of mycaminosyl tylonolide (1) and 4'-deoxymycaminosyl tylonolide (2) containing N-ethyl-2-fluoro-, 2, 2-difluoro- and 2, 2, 2-trifluoroethylamino groups at their C-23 have been prepared by treating 23-deoxy-23-ethylaminomycaminosyl tylonolide diethyl acetal (14) and its 4'-deoxy analog 15 with 2-fluoro-, 2, 2-difluoro- and 2, 2, 2-trifluoroethyl trifluoromethanesulfonates. The relationship between the antibacterial activity and the numbers of the fluorine atoms introduced in the final products is discussed.
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  • DEREK HORTON, WALDEMAR PRIEBE, OSCAR VARELA
    1984 Volume 37 Issue 12 Pages 1635-1641
    Published: 1984
    Released: April 19, 2006
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    3'-Deamino-4'-epi-3'-hydroxy-daunorubicin (11) and -doxorubicin (14) have been synthesized.In the in vivo murine P-388 lymphocytic leukemia assay, these two compounds were more active than daunorubicin (1) and doxorubicin (2), respectively. Comparative studies in the P-388 assay indicated 3'-deamino-3'-hydroxydoxorubicin (3) to be more active than its 4'-epimer 14.
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  • BERNARD J. GRAVES, DONALD B. BOYD, KENNY B. LIPKOWITZ
    1984 Volume 37 Issue 12 Pages 1642-1650
    Published: 1984
    Released: April 19, 2006
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    Molecular orbital calculations by the CNDO/2D method yield charge distributions which correlate well with the observed 13C NMR chemical shift for the amide carbon of acylamino side chains of cephalosporins. Acyl groups that withdraw electrons from the amide C-N bond and concomitantly make the amide nitrogen more negatively charged increase the chemical shift. The trends are related to the degree of amide resonance. No direct correlation was found between the chemical shift of the amide carbon and the antibacterial activity of the cephalosporins.
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  • HWEI-RU TSOU, SRINIVASAN RAJAN, RUTH FIALA, PATRICK C. MOWERY, MILON W ...
    1984 Volume 37 Issue 12 Pages 1651-1663
    Published: 1984
    Released: April 19, 2006
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    The biosynthesis of maduramicin α and β in a culture of Actinomadura yumaensis has been studied using 13C, 14C and 18O labeled precursors. The α component of this recently discovered polyether antibiotic, containing forty-seven carbon atoms in a seven-ring system, is derived from eight acetate, seven propionate and four methionine molecules. The β component which is missing one methoxy group incorporates three methionine methyl groups. The carbohydrate moiety was enriched by methionine, but not significantly by acetate or propionate. Studies of the incorporation of 13C labeled precursors permit the 13C NMR assignment of maduramicin. The origin of oxygen atoms of maduramicin has been examined by feeding [1-13C, 18O2]acetate and [1-13C, 18O2]propionate separately in the fermentation culture and the resulting doubly labeled maduramicin samples were analyzed by the isotopic shifts in the 13C NMR spectra. These results are consistent with the initial formation of a triene, which is converted to maduramicin by cyclization of the triepoxide.
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  • SEIGA ITOH, YOSHIHIRO ODAKURA, HIROSHI KASE, SEIJI SATOH, KEIICHI TAKA ...
    1984 Volume 37 Issue 12 Pages 1664-1669
    Published: 1984
    Released: April 19, 2006
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    Biosynthesis of astromicin, a unique pseudodisaccharide aminoglycoside antibiotic containing 1, 4-diaminocyclitol component, was investigated by isolating a variety of possible precursor compounds from mutants of Micromonospora olivasterospora in which biosynthetic pathways for astromicin were blocked. Washed mycelia of M. olivasterospora mutants converted these compounds to astromicin, which was detected by thin-layer chromatography. Since astromicin possesses one glycyl and three methyl groups, [14C]glycine and [14C]methionine should be incorporated into precursors to form astromicin. To confirm the biosynthetic pathway, formation of labeled astromicin from the precursors was examined using [1-14C]-glycine or [methyl- 14C ]methionine. From above results, we propose the biosynthetic pathway for astromicin as shown in Fig. 2.
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  • YOSHIHIRO ODAKURA, HIROSHI KASE, SEIGA ITOH, SEIJI SATOH, SEIGO TAKASA ...
    1984 Volume 37 Issue 12 Pages 1670-1680
    Published: 1984
    Released: April 19, 2006
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    An inosamine-idiotrophic mutant, KY11559, which produced no astromicin unless scyllo-inosamine was added to the fermentation medium, was isolated from Micromonospora olivasterospora. Biotransformation studies were performed with resting cells of this mutant and compounds assumed to be precursors of 1, 4-diaminocyclitol (fortamine). Scyllo-inosose, scyllo-inosamine and FU-10 were converted to astromicin. A number of mutants blocked in the biosynthesis of astromicin were developed from M. olivasterospora, and the intermediates accumulated by these mutants were isolated and identified. Twenty-five blocked mutants were classified into 10 groups, based on their complementation patterns by cosynthesis experiments. Further, utilizing these blocked mutants and the isolated compounds, biotransformation analyses were performed. The results showed that the amination at position 4 in fortamine occurred after formation of the pseudodisaccharide. Subsequently, the aminosugar and aminocyclitol moieties were aminated, methylated, dehydroxylated, epimerized and acylated to produce astromicin. Thus it was demonstrated that the astromicin biosynthetic pathway has a unique feature which is not found in the biosynthesis of other aminoglycoside antibiotics.
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  • KOZO KITAURA, KAZUYUKI MINEURA, NOBUHIRO NAKAMIZO, KHOYA SHIBA, MASAHI ...
    1984 Volume 37 Issue 12 Pages 1681-1686
    Published: 1984
    Released: April 19, 2006
    JOURNALS FREE ACCESS
    Synergistic activity of astromicin and an antipseudomonal β-lactam antibiotic such as piperacillin, cefsulodin or carbenicillin againstPseudomonas aeruginosawas demonstratedin vitroandin vivo. Synergyin vitrowas observed more often when astromicin was combined with piperacillin or cefsulodin than when it was combined with carbenicillin. The combination of astromicin with piperacillin showed a bactericidal activity againstPseudomonas aeruginosaat a bacteriostatic concentration of each antibiotic alone. The synergy observedin vitrowas reproduced against experimental mouse infections, and the astromicin-piperacillin or cefsulodin combination produced significantly greater protective effects than the single use of individual antibiotics.
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  • TOSHIRO MOROHOSHI, MINORU TORIYA, SHIGEYUKI YOKOIYAMA, KENTARO FUJIMOT ...
    1984 Volume 37 Issue 12 Pages 1687-1691
    Published: 1984
    Released: April 19, 2006
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    It was found that Serratia marcescens 43, Serratia proteamaculans 48 and Serratia sp. 45, all of which were clinically isolated, produced a new type of aminoglycoside acetyltransferase which acetylated amikacin at the 6'-amino group. 1-N-[(S)-3-Amino-2-hydroxypropionyl]-gentamicin B (HAPA-B, SCH 21420) and gentamicin C2 were hardly inactivated by the enzymes and had effective antimicrobial activities against these strains both in vitro and in vivo. This kind of aminoglycoside acetyltransferase should be classified into a new group other than previously reported AAC(6') enzymes.
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  • P. BARTHÉLÉMY, D. AUTISSIER, G. GERBAUD, P. COURVALIN
    1984 Volume 37 Issue 12 Pages 1692-1696
    Published: 1984
    Released: April 19, 2006
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    Escherichia coli BM2195 is highly resistant to erythromycin by inactivation of the antibiotic. We have determined the structure of the modified antibiotic by physico-chemical techniques including mass spectrometry, infrared spectrophotometry, 13C nuclear magnetic resonance, and circular dichroism. The results obtained indicate that E. coli BM2195 resists erythromycin by the production of an erythromycin esterase which hydrolyzes the lactone ring of the antibiotic.
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  • SETSUKO KUNIMOTO, KEIKO MIURA, KAZUO UMEZAWA, CHIN-ZHI XU, TORU MASUDA ...
    1984 Volume 37 Issue 12 Pages 1697-1702
    Published: 1984
    Released: April 19, 2006
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    Cellular uptake and cytostatic activity of 4'-O-tetrahydropyranyladriamycin (THP) in various sublines resistant to anthracycline antibiotics of mouse lymphoblastoma L5178Y, Chinese hamster ovary (CHO) and mouse leukemia P388 cells were studied. All the sublines resistant to adriamycin (ADR) showed slightly decreased uptake of THP as compared with each sensitive lines, but THP was still taken up much more quickly than ADR by each of the ADR-resistant cell lines. Efflux of both anthracycline glycosides from the ADR-resistant P388 cells was faster than that from the ADR-sensitive P388 cells. The percentage of THP retained at equilibrium was higher than that of ADR in both ADR-resistant and -sensitive P388 cells. Cytotoxicity of THP to ADR-resistant cell lines was considerably lower compared with that for each of the sensitive lines but THP inhibited growth of ADR-resistant tumor cells at a concentration about 10 times lower than that for ADR. Thus THP was taken up more quickly, effluxed more slowly than ADR from the ADR-resistant cells, and showed stronger cytostatic activity than ADR on the cells.
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  • KATSUMI KAWAHARAJO, KEIKO SHITOH, MASAHIRO NIIZEKI, SHINJIRO MURATA, Y ...
    1984 Volume 37 Issue 12 Pages 1703-1711
    Published: 1984
    Released: April 19, 2006
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    The antibacterial activity of MT-141 against Escherichia coli and Proteus morganii in compromised mice was investigated and compared with that of latamoxef, cefmetazole and cefoxitin. The bactericidal activity of MT-141 in short-term contact with E. coli and P. morganii was markedly enhanced when combined with mouse serum, and the activity of MT-141 was greater than the activities of the three reference drugs. The antibacterial activities of MT-141 in the liver, spleen and kidney of neutropenic and Sarcoma 180 tumor-bearing mice infected with E. coli and P. morganii were superior to the activities of the reference drugs. MT-141 was more potent than cefmetazole and cefoxitin, and similar to latamoxef in potency against systemic P. morganii infection in Sarcoma 180 tumor-bearing mice.
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  • N. KLESEL, M. LIMBERT, G. SEIBERT, I. WINKLER, E. SCHRINNER
    1984 Volume 37 Issue 12 Pages 1712-1718
    Published: 1984
    Released: April 19, 2006
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    The activity of the aminothiazolyliminomethoxy cephalosporin cefodizime (HR 221) was compared to that of cefotaxime, cefuroxime and cefazolin in experimental pneumonia caused by Klebsiella pneumoniae DT-S in mice. Cefodizime exhibited high and long-acting levels in the blood and lung homogenates of infected mice; the blood and tissue concentrations obtained with the other cephalosporins tested were low by comparison. In the treatment of experimental Klebsiella pneumonia, cefodizime was superior to cefotaxime and cefuroxime. Counts of the number of viable bacteria present in the infected tissue showed that cefodizime exerted a more marked bactericidal effect than cefotaxime or cefuroxime. Hardly any therapeutic activity was seen with cefazolin.
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  • MICHAEL LIMBERT, ROBERT R. BARTLETT, GERHARD DICKNEITE, NORBERT KLESEL ...
    1984 Volume 37 Issue 12 Pages 1719-1726
    Published: 1984
    Released: April 19, 2006
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    Studies concerning the activity of cefodizime (HR 221), on certain aspects of the immune response, were conducted. It was found that lymphocytes from Balb/c mice treated with 3 and 30 mg/kg/day of cefodizime display increased responsiveness to B-cell mitogens and specific antigens. Also, the amount of antigen specific antibody producing plaque forming cells was increased in these mice and was accompanied by a rise in the specific IgG haemagglutinin titer. These effects were not observed in lymphocytes obtained from NMRI mice that had been treated with cefodizime. Peritoneal macrophages from NMRI mice, treated with cefodizime prior to harvesting of the cells, contained increased levels of lysosomal enzymes, developed enhanced chemiluminescent reaction to stimuli and showed elevated pinocytosis rates. Furthermore, NMRI mice treated with cefodizime during the immunization, developed enhanced DTH-reaction, when challenged with the antigen (SRBC). The prophylactic treatment of Balb/c mice with cefodizime (2×30 mg/kg/day ip for 4 days) significantly prolonged the mean survival time of the animals after intravenous infection with Candida albicans 200/175 (16.7 days as against 3.5 days in the case of the controls). This stimulatory effect of cefodizime on the host defence system was not observed for NMRI mice. Treatment with latamoxef or cefoperazone under the same experimental conditions did not reduce the susceptibility of mice to C. albicans. The protective activity of cefodizime against C. albicans in Balb/c mice, may be due to the immuno-stimulatory activity of this agent.
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  • MICHAEL D. JONES, JEFFREY T. FAYERMAN
    1984 Volume 37 Issue 12 Pages 1727-1728
    Published: 1984
    Released: April 19, 2006
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  • RONALD A. DIXON, ROBERT A. EDMONDSON, KENNETH D. HARDY, PETER H. MILNE ...
    1984 Volume 37 Issue 12 Pages 1729-1731
    Published: 1984
    Released: April 19, 2006
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  • RONALD A. DIXON, KENNETH D. HARDY, ARUN C. KAURA, PETER H. MILNER, AND ...
    1984 Volume 37 Issue 12 Pages 1732-1735
    Published: 1984
    Released: April 19, 2006
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  • HIROYOSHI TOHYAMA, TATSURO SHIGYO, YOSHIRO OKAMI
    1984 Volume 37 Issue 12 Pages 1736-1737
    Published: 1984
    Released: April 19, 2006
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  • J. W. WESTLEY, C.-M. LIU, L. H. SELLO, R. H. EVANS, N. TROUPE, J. F. B ...
    1984 Volume 37 Issue 12 Pages 1738-1740
    Published: 1984
    Released: April 19, 2006
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  • SHOKICHI OHUCHI, HIROYUKI SUDA, HIROSHI NAGANAWA, KENJI KAWAMURA, TAKA ...
    1984 Volume 37 Issue 12 Pages 1741-1743
    Published: 1984
    Released: April 19, 2006
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