The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Volume 54 , Issue 10
Showing 1-14 articles out of 14 articles from the selected issue
  • LÁSZLÓ VÉRTESY, HERBERT KOGLER, ASTRID MARKUS, MA ...
    2001 Volume 54 Issue 10 Pages 771-782
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    The terpene peptide memnopeptide A (1), C76H108N16O18S, MW 1564, was isolated from a culture of the fungus Memnoniella echinata FH 2272 on casein peptone. The structure of the novel compound was elucidated with the aid of 2D NMR experiments and from amino acid analysis and mass spectrometric sequencing of the peptide. The compound consists of a known phenylspirodrimane subunit linked to the decapeptide Met-His-Gln-Pro-His-Gln-Pro-Leu-Pro-Pro. This proline-rich peptide is a subsequence of β-casein. From the observed absence in the literature of any other highly significant sequence homologues, memnopeptide A can be assumed to arise from metabolic products of the fungus with direct incorporation of constituents of the nutrient medium. The formation of memnopeptide A suggests this may be a mechanism for storage of amines by the fungus. Memnopeptide A has weak antibacterial activity against Gram-positive bacteria and effects half-maximal activation of sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA2) at a concentration of 12.5 μm.
    Download PDF (851K)
  • LIN NIE, MASASHI UEKI, HIDEAKI KAKEYA, HIROYUKI OSADA
    2001 Volume 54 Issue 10 Pages 783-788
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    We have developed a novel p21WAF1 promoter activator screening system based on rapid and facile luciferase activity assay of a model cell system (H1299/tsp53-luc cells), a stable luciferase expression cell line established by transfecting H1299/tsp53 cells with a reporter gene construct pWWP-Luc-BSD. This plasmid was constructed by subcloning the 2.4kb p21WAF1 promoter and a 2.6kb of luciferase cDNA fragment activated by the p21WAF1 promoter into a pMAM2-BSD expression vector containing the blasticidin S deaminase gene (BSD). A BSD-resistant clone H1299/tsp53-luc#4, showing the highest response to p53 activation (by temperature shift from 37°C to 32°C) by luciferase production, was used for screening microbial culture broths. Among approximately 1200 screened samples, trichostatin A related compounds and a new compound, lucilactaene, were isolated. This provides an effective and facile screening system for p21WAF1 promoter activators which should be of considerable value in the rapid identification of new anticancer agents.
    Download PDF (436K)
  • KOHKI ENDO, KEIKO KAMO, KUNIAKI HOSONO, TERUHIKO BEPPU, KENJI UEDA
    2001 Volume 54 Issue 10 Pages 789-796
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Nitrosoguanidine-induced melanin-negative mutants of Streptomyces griseus were classified according to their ability to produce streptomycin and A-factor, and to form aerial mycelium. A large proportion of the mutants showed deficiency in either antibiotic production and morphological development or both, suggesting close regulatory correlation between melanogenesis and morphological and physiological differentiation. The tyrosinase-encoding mel operon of S. griseus was cloned and examined for its role in melanogenesis of this organism. As in other Streptomyces homologues, the operon consisted of two open reading frames, melCl encoding the putative cofactor and melC2 encoding the tyrosinase. Regardless of the distinct sequence similarity, introduction of the operon on plasmids failed to confer melanin production in the melanin-negative mutants, and the disruption of melC2 barely affected the melanin productivity, which indicated the presence of another enzyme involved in the melanogenesis in S. griseus. On the other hand, mel on a high-copy-number plasmid caused precocious aerial mycelium formation in Streptomyces lividans TK21 suggesting a stimulatory role of tyrosinase in morphological development.
    Download PDF (825K)
  • MARTIN KRAUSE, ANTJE LINDEMANN, MIRKO GNNSKI, TILL HORNBOGEN, GERHARD ...
    2001 Volume 54 Issue 10 Pages 797-804
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    New cyclohexadepsipeptides of the enniatin type with potential anthelmintic properties were produced by two different strategies: 1. In vitro synthesis by use of the multienzyme enniatin synthetase, and 2. in vivo precursor feeding of enniatin producing strains Fusarium scirpi and Fusarium sambucinum. The compounds were analyzed by HPLC, various NMR measurements and mass spectrometry. The three N-methyl L-amino acid positions in the enniatin B molecule could be gradually replaced by other (N-methyl) L-amino acids, e.g. alanine, cysteine, threonine and serine. The latter two amino acids yield new enniatins with functional groups in the hydrophobic side chains. Similarly the three D-2-hydroxyisovalerate residues, present in all naturally occuring enniatins, could be substituted by D-2-hydroxybutyric acid and D-lactic acid. Despite its lower yield the in vitro synthesis has the advantage of a broader variety of products formed.
    Download PDF (692K)
  • KAN FANG, GERHARD SCHLINGMANN, ANNMARIE ENOS, GUY T. CARTER
    2001 Volume 54 Issue 10 Pages 805-809
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Selected nemadectins (formerly LL-F28249 series1)) have been fed to a panel of microorganisms with the aim of generating new derivatives. In addition to products resulting from the oxidation of the terminal methyl group (C-29), a unique phosphorylated nemadectin was isolated. The phosphate group was determined to be at C-23 by HMBC between phosphorus and H-23. Milbemycin or nemadectin derivatives with natural substituents involving the 23-hydroxyl group were hitherto unknown.
    Download PDF (322K)
  • TETSUO KISO, YOSHINOSUKE USUKI, XU PING, KEN-ICHI FUJITA, MAKOTO TANIG ...
    2001 Volume 54 Issue 10 Pages 810-817
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    L-2, 5-Dihydrophenylalanine (DHPA), a phenylalanine analogue, induced apoptosis in human promyelocytic leukemia cells (HL-60). This apoptosis was demonstrated by morphological changes of the cells, such as fragmentation of nuclei and chromatin condensation, and by some evidence found in biochemical analysis, such as DNA ladder and activation of caspase 3. The DHPA-induced apoptosis was prevented by a pan-caspase inhibitor, Z-VAD-fmk, and a cysteine protease inhibitor, E-64d, which inhibits calpains and camepsin B and L. A calpain inhibitor, Z-LL-H, did not affect this apoptosis. A cathepsin B specific inhibitor, CA074-Me, prevented only chromatin condensation. However, E-64d and a cathepsin L specific inhibitor, Z-FY(t-Bu)-dmk, protected the cells from both chromatin condensation and oligonucleosomal DNA fragmentation. As proceeding to the apoptotic process, the activities of both cathepsin B and L increased gradually. These results indicated that DHPA was an inducer of cathepsin-dependent apoptosis in HL-60 cells.
    Download PDF (723K)
  • HARUMI CHIBA, SATOSHI ASANUMA, MEGUMI OKAMOTO, JUNJI INOKOSHI, HARUO T ...
    2001 Volume 54 Issue 10 Pages 818-826
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    The first step in cellular entry of HIV involves binding of the viral envelope glycoprotein complex (gp120/gp41) to specific receptor molecules on the target cells. The cell-cell fusion (syncytium formation) between env expressing cells and CD4+ cells mimics the viral infection of the host cells. To search for anti-HIV substances preventing this process, we constructed the recombinant cell lines, HeLa/CD4/Lac-Z and HeLa/T-env/Tat for T-cell tropic (HIV-1NL4-3) system, and HOS/CD4/CCR5/Lac-Z and HeLa/M-env/Tat for macrophage tropic (HIV-1SF162) system. When each pair of cells were co-incubated for 20 hours, the multinuclear giant cells (syncytia) were formed and β-galactosidase was expressed. These systems are less biohazardous because no infectious virus particles are used. Their validity in screening for anti-HIV substances which inhibit syncytium formation was confirmed using various known HIV entry inhibitors.
    Download PDF (1132K)
  • WON-SUCK SUN, HYO-SUNG LEE, JUNG-MIN PARK, SUNG-HAN KIM, JU-HYUN YU, J ...
    2001 Volume 54 Issue 10 Pages 827-830
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    A series of novel N-substituted tyramine (2-p-hydroxyphenylethylamine) derivatives (1-11) were synthesized and evaluated for their inhibitory activity against pig kidney aldose reductase (EC 1, 1, 1, 21). Of these compounds, N-2-p-hydroxyphenylethyl maleamic acid (10) exhibits the strongest aldose reductase inhibitory activity, which is 22 times more potent than that of YUA0011).
    Download PDF (272K)
  • WON-GON KIM, NAN-KYU SONG, ICK-DONG YOO
    2001 Volume 54 Issue 10 Pages 831-835
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Download PDF (331K)
  • RYUICHIRO NAKAI, SHINGO KAKITA, AKIRA ASAI, SHIGERU CNIBA, SHIRO AKINA ...
    2001 Volume 54 Issue 10 Pages 836-839
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Download PDF (210K)
  • SANG B. HANR, HO J. LEE, YUNG H. KHO, YOUNG J. JEON, SANG H. LEE, HYUN ...
    2001 Volume 54 Issue 10 Pages 840-843
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Download PDF (413K)
  • DAVID BARRETT, AKIRA TANAKA, ETSUKO WATABE, KATSUYUKI MAKI, FUMIAKI IK ...
    2001 Volume 54 Issue 10 Pages 844-847
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Download PDF (240K)
  • K. C. SEKHAR RAO, S. DIVAKAR, N. G. KARANTH, A. P. SATTUR
    2001 Volume 54 Issue 10 Pages 848-849
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Download PDF (138K)
  • HIDEAKI KAKEYA, SHUN-ICHIRO KAGEYAMA, LIN NIE, RIE ONOSE, GEN OKADA, T ...
    2001 Volume 54 Issue 10 Pages 850-854
    Published: October 25, 2001
    Released: September 19, 2008
    JOURNALS FREE ACCESS
    Download PDF (296K)
feedback
Top