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Volume 40, Issue 10
Displaying 1-20 of 20 articles from this issue
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I. TAXONOMY OF THE PRODUCING ORGANISM, FERMENTATION, ISOLATION AND BIOLOGICAL ACTIVITYCHRISTOPHER M. M. FRANCO, JULIA N. GANDHI, SUGATA CHATTERJEE, BIMAL N. ...1987 Volume 40 Issue 10 Pages 1361-1367
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSA new macrolide antibiotic, swalpamycin, has been isolated from the culture broth of Streptomyces sp. Y-84, 30967. Taxonomically the producing organism most closely resembles Streptomyces anandii and has therefore been named S. anandii subsp. swalpus. Swalpamycin is a neutral 16-membered macrolide active against Gram-positive bacteria including erythromycin-resistant strains.View full abstractDownload PDF (573K) -
II. STRUCTURE ELUCIDATIONSUGATA CHATTERJEE, GOUKANAPALLI C. S. REDDY, CHRISTOPHER M. M. FRANCO, ...1987 Volume 40 Issue 10 Pages 1368-1374
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSA new antibiotic swalpamycin (1) has been isolated from the culture broth of Streptomyces sp. Y-84, 30967. The antibiotic has the molecular formula of C37H56O14 and belongs to the class of 16-membered neutral macrolide antibiotics. Its structure has been elucidated by an analysis of its spectral properties. It contains a novel aglycone herein called swalpanolide.View full abstractDownload PDF (384K) -
I. TAXONOMY, FERMENTATION AND BIOLOGICAL PROPERTIESMARIANNA JACKSON, JAMES P. KARWOWSKI, ROBERT J. THERIAULT, PRABHAVATHI ...1987 Volume 40 Issue 10 Pages 1375-1382
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSColoradocin was discovered in a screen for anti-anaerobe activity. The producing organism was determined to be a new species of Actinoplanes, designated Actinoplanes coloradoensis sp. nov. Coloradocin inhibits Bacteroides, Clostridium and other anaerobes. It does not inhibit most aerobic bacteria but is effective against Neisseria gonorrhoeae and Haemophilus influenzae. Coloradocin has low acute toxicity.View full abstractDownload PDF (500K) -
II. IDENTITY WITH LUMINAMICIN AND ELUCIDATION OF STRUCTURERONALD R. RASMUSSEN, MICHAEL H. SCHERR, DAVID N. WHITTERN, ALEX M. BUK ...1987 Volume 40 Issue 10 Pages 1383-1393
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSColoradocin was isolated from a fermentation broth by adsorption onto Amberlite XAD-2. The activity was eluted in MeOH and purified by gel filtration on Shephadex LH-20, followed by liquid-liquid chromatography on diol-bonded silica gel. The last two steps in the purification of this antibiotic included reverse-phase chromatography on CIS-bonded silica gel and countercurrent chromatography on an Ito Coil Planet Centrifuge to give material of 90% purity. Analytically pure material was obtained by preparative HPLC. As a result of extensive homo and heteronuclear two-dimensional NMR studies, a structure was proposed for coloradocin. The structure consists of a decalin ring system fused to a 10-membered macrolactone which in turn is fused to a 14-membered macrolactone possessing an enol ether in conjugation with an unsaturated cyclic anhydride functionality. Coloradocin is related to a small class of antibiotics which include nodusmicin and nargenicin and was shown to be identical to luminamicin for which no structure has been reported.View full abstractDownload PDF (558K) -
I. TAXONOMY, FERMENTATION, ISOLATION AND CHARACTERIZATIONMOTOAKI NISHIKAWA, YASUHISA TSURUMI, TAKAYUKI NAMIKI, KEIZO YOSHIDA, M ...1987 Volume 40 Issue 10 Pages 1394-1399
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSWF-3681 was isolated from a cultured filtrate of Chaetomella raphigera as a novel inhibitor of aldose reductase. It was extracted with ethyl acetate and then purified with silica gel chromatography. Its molecular formula was determined to be C13H12O5 by elemental analysis and high resolution electron impact mass spectrometry. IC50 of WF-3681 was 2.5×10-7 M for partially purified aldose reductase of rabbit lens.View full abstractDownload PDF (560K) -
II. STRUCTURE DETERMINATION AND SYNTHESISTAKAYUKI NAMIKI, MOTOAKI NISHIKAWA, YOSHIKUNI ITOH, ITSUO UCHIDA, MASA ...1987 Volume 40 Issue 10 Pages 1400-1407
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSThe structure of WF-3681, a novel aldose reductase inhibitor produced by Chaetomella raphigera Swift No. 3681, was deduced to be 1 on the basis of its spectroscopic and chemical evidences and confirmed by a total synthesis starting from (E)-5-phenyl-4-pentenol.View full abstractDownload PDF (566K) -
FERMENTATION, ISOLATION AND STRUCTURE ELUCIDATIONMAHESH PATEL, VINCENT P. GULLO, VINOD R. HEGDE, ANN C. HORAN, FRANK GE ...1987 Volume 40 Issue 10 Pages 1408-1413
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSA novel tetracycline antibiotic, Sch 33256, was isolated from a culture broth of a new species of Actinomadura. The antibiotic was isolated by solvent extraction, Sephadex G-25 column chromatography and crystallization. The structure was determined by comparison of the spectra with that of chlortetracycline. Spectroscopic analysis of the compound yielded 2'-N-methyl-8-methoxychlortetracycline as the proposed structure.View full abstractDownload PDF (290K) -
FERMENTATION, ISOLATION AND STRUCTURE ELUCIDATIONMAHESH PATEL, VINCENT P. GULLO, VINOD R. HEGDE, ANN C. HORAN, JOSEPH A ...1987 Volume 40 Issue 10 Pages 1414-1418
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSAn actinomycete identified as a Dactylosporangium sp. produces a new tetracycline, 4ahydroxy-8-methoxychlortetracycline (Sch 34164). The addition of magnesium ions to complex fermentation media increased the antibiotic titers. Sch 34164 was isolated by solvent extraction and Sephadex G-25 column chromatography. The novel structure was proposed based on spectroscopic analysis. The shift of C-4a (35 to 77 ppm) and C-8 (140 to 163 ppm) in the 13C NMR as compared to chlortetracycline was indicative of the novel hydroxyl and methoxy substituents, respectively.View full abstractDownload PDF (246K) -
ISOLATION OF A THIRD 8-METHOXY SUBSTITUTED CHLORTETRACYCLINEELIZABETH B. SMITH, HANAN K. MUNAYYER, MICHAEL J. RYAN, B. ALLEN MAYLE ...1987 Volume 40 Issue 10 Pages 1419-1425
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSActinomadura brunnea produces 2'-N-methyl-8-methoxychlortetracycline. A derivative of this strain has been isolated that is specifically blocked in methylation of the 2'-amino position. This isolate was detected by screening approximately 30, 000 colonies of a mutagenized population of Actinomadura brunnea using a direct soft agar overlay with an Escherichia coli indicator. The antibiotic produced by the blocked mutant has been identified as 8-methoxychlortetracycline (Sch 36969) based upon its biological activity, relative mobility on TLC and HPLC, and spectroscopic data.View full abstractDownload PDF (607K) -
A. CACCIAPUOTI, E. L. Moss, F. MENZEL, C. ADAM CRAMER, W. WEISS, D. LO ...1987 Volume 40 Issue 10 Pages 1426-1430
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSThe in vitro activities of three new 8-methoxychlortetracyclines, Sch 36969, 33256 and 34164 were compared to tetracycline, minocycline and doxycycline. Against aerobic Gramnegative rods Sch 36969 had a geometric mean MIC (GMM) of 4.2 μg/ml, about 8-fold more potent than Sch 33256, and similar to all the other compounds. Sch 36969 also had good activity against methicillin-resistant (GMM, 0.21 μg/ml) and -susceptible Staphylococci (GMM, 0.14 μg/ml), Streptococci (GMM, 0.06 μg/ml), and most anaerobic bacteria (GMM, <0.5 μg/ml). In general, Sch 36969 was similar to, or more potent than, all the other compounds tested. Serum levels of Sch 36969 in squirrel monkeys were 4-fold lower (AUC, 4.5 μg•hours/ml) than those of chlortetracycline (AUC, 16.1 μg•hours/ml). In mouse protection tests (PD50s) against various strains of bacteria, Sch 36969 was similar in activity to tetracycline, but up to 6-fold less active than chlortetracycline. The structure activity relationships for these new chlortetracyclines are described.View full abstractDownload PDF (306K) -
BACIMETHRIN ISOLATED FROM STREPTOMYCES ALBUS IDENTIFICATION, DERIVATIVES, SYNTHESIS AND BIOLOGICAL PROPERTIESHANNELORE DRAUTZ, WERNER MESSERER, HANS ZÄHNER, SABINE BREIDING-M ...1987 Volume 40 Issue 10 Pages 1431-1439
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSBacimethrin (1), known as a thiamine antagonist produced by Bacillus megatherium, was isolated from Streptomyces albus and has been further characterized by NMR spectra and acetylation. A new easy three step synthesis for 1 is described. The biological activity of 1, and its mode of action were discussed. There are indications that bacimethrin inhibits the phosphorylation of 4-amino-5-hydroxymethyl-2-methylpyrimidme (Pyr-OH) during thiamine biosynthesis.View full abstractDownload PDF (583K) -
TAICHI MANOME, EMIKO HOSHINO1987 Volume 40 Issue 10 Pages 1440-1447
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSThe thiostrepton-resistance gene is expressed in Streptomyces jumonjinensis [16]-8 SANK 61185 carrying the plasmid pIJ702 but not the tyrosinase gene. We have isolated DNA fragments from various streptomycetes that restore expression of the tyrosinase gene on pIJ702 in this organism. The nucleotide sequences of two of these DNA fragments show that they contain putative ribosome binding sites and -10 regions of possible promoters.View full abstractDownload PDF (657K) -
KYUICHI NEMOTO, MICHIKO HAYASHI, JUNPEI ITO, FUMINORI ABE, TOMOHISA TA ...1987 Volume 40 Issue 10 Pages 1448-1451
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSWe investigated the effect of spergualin (SGL) upon the development of spontaneous systemic lupus erythematosus-like lesions in male MRL/MpJ-lpr/lpr mice. SGL was administered ip at doses of 2.5, 5 and 10 mg/kg to two groups of mice. One group received SGL prophylactically from 7 to 21 weeks of age. The other group received SGL curatively from 13 to 27 weeks of age. The occurrence of lupus lesions was characterized by enlarged lymphoid organs, high anti-DNA titer and blood urea nitrogen, and severe glomerular nephritis. In both groups these characteristics were significantly suppressed by SGL in a dosedependent manner. This inhibitory activity was greatest at a dose of 10 mg/kg. These findings suggest that SGL has prophylactic and curative effects against lupus lesions in autoimmune disease in mice.View full abstractDownload PDF (228K) -
ICHIHIKO YAMAMOTO, MASAYA NAKAGAWA, YOICHI HAYAKAWA, KAZUYOSHI ADACHI, ...1987 Volume 40 Issue 10 Pages 1452-1454
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSDownload PDF (133K) -
HUBERT MAEHR, RALPH H. EVANS1987 Volume 40 Issue 10 Pages 1455-1456
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSDownload PDF (96K) -
HIROSHI TOMODA, KAZUAKI IGARASHI, YOSHITAKE TANAKA, SATOSHI OMURA1987 Volume 40 Issue 10 Pages 1457-1460
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSDownload PDF (234K) -
KUNIO ISSHIKI, YOSHIKAZU TAKAHASHI, HIRONOBU IINUMA, HIROSHI NAGANAWA, ...1987 Volume 40 Issue 10 Pages 1461-1463
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSDownload PDF (156K) -
TAKAYUKI USUI, SUMIO UMEZAWA1987 Volume 40 Issue 10 Pages 1464-1467
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSDownload PDF (195K) -
YOSHIO HAMASHIMA, TADATOSHI KUBOTA, KYOJI MINAMI, KOJI ISHIKURA, TOSHI ...1987 Volume 40 Issue 10 Pages 1468-1470
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSDownload PDF (166K) -
MASAYA IMOTO, KAZUO UMEZAWA, KUNIO ISSHIKI, SETSUKO KUNIMOTO, TSUTOMU ...1987 Volume 40 Issue 10 Pages 1471-1473
Published: October 25, 1987
Released on J-STAGE: April 19, 2006
JOURNAL FREE ACCESSDownload PDF (205K)
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