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KAYO AOYAGI, FUMINORI ABE, KYUICHI NEMOTO, SHIGERU ABE, MASAAKI ISHIZU ...
1994 Volume 47 Issue 10 Pages
1077-1083
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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An analogue of deoxyspergualin, N-563 has an immunostimulating activity whereas the mother compound has been found to be a potent immunosuppressant. In this study, the protective effect of the analogue against
C. albicans infection was investigated in normal and immunosuppressed mice. In normal mice, N-563 treatment at 10 mg/kg for 3 days prior to infection significantly prolonged the survival time. In immunosuppressed mice treated with a single dose of cyclophosphamide 4 days prior to infection, N-563 at 3 and 10 mg/kg for 3 days prior to infection also significantly prolonged the survival time of mice. In addition, it augmented the phagocytic activity of neutrophils and enhanced the delayed type hypersensitivity reaction against
C. albicans. Coincidentally, N-563 appeared to protect against secondary infection with
C. albicans in the delayed type hypersensitivity-positive mice.
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I. TAXONOMY, FERMENTATION, ISOLATION AND PHYSICO-CHEMICAL PROPERTIES
TOSHIRO IWAMOTO, AKIHIKO FUJIE, KAZUTOSHI SAKAMOTO, YASUHISA TSURUMI, ...
1994 Volume 47 Issue 10 Pages
1084-1091
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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WF 11899A, B and C, novel antifungal lipopeptide antibiotics were isolated from the culture broth of
Coleophoma empetri F-11899. These compounds belong to the echinocandin type of lipopeptides. Of these compounds, WF11899 A showed good solubility in water. These three antibiotics possess potent
in vitro antifungal activities against
Candida spp.
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II. BIOLOGICAL PROPERTIES
TOSHIRO IWAMOTO, AKIHIKO FUJIE, KUMIKO NITTA, SEIJI HASHIMOTO, MASAKUN ...
1994 Volume 47 Issue 10 Pages
1092-1097
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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WF11899A, B and C, novel water-soluble lipopeptides related to the echinocandins, possess potent anti-
Candida activities. The IC
50s of the compounds against four clinical isolates of
Candida albicans ranged from 0.004 to 0.03 μg/ml by microbroth dilution assay. These compounds mildly suppressed the growth of
Aspergillus fumigatus and
A. niger. WF11899A, B and C showed a potent
in vivo anti-
Candida activity. Particularly, WF11899A was superior to cilofungin, and equal to fluconazole. 1, 3-β-glucan synthase was inhibited by these compounds at the IC
50s of 0.7, 0.7 and 1.8μg/ml for WF 11899A, B and C, respectively. However, they hemolysed mouse red blood cells
in vitro at the concentration of 62 μg/ml.
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FRANK PETERSEN, THÉOPHILE MOERKER, FORTUNATO VANZANELLA, HEINRI ...
1994 Volume 47 Issue 10 Pages
1098-1103
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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Cladospirone bisepoxide (
1), a novel metabolite, was isolated from cultures of a fungus which was characterized as a coelomycete by the formation of pycnidia. By optimization of media and fermentation conditions, a titer of up to 1.5g/liter on shake level and 1.16g/liter on bioreactor scale could be achieved. The isolation of the compound was performed by solvent extraction of the culture broth and subsequent crystallization. Cladospirone bisepoxide displays selective antibiotic activity against several bacteria and fungi and inhibits germination of
Lepidium sativum at low concentrations.
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I. TAXONOMY, FERMENTATION, ISOLATION, PHYSICO-CHEMICAL AND BIOLOGICAL PROPERTIES
KEN-ICHIRO HAYASHI, MASAHIRA NAKAGAWA, MITSURU NAKAYAMA
1994 Volume 47 Issue 10 Pages
1104-1109
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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Nisamycin, a new manumycin group antibiotic, was isolated from the culture broth of
Streptomyces sp. K106. This strain was designated to genus
Streptomyces by the taxonomic features. Nisamycin was purified by ethyl acetate extraction, silica gel column chromatography, preparative silica gel TLC, and Sephadex LH-20 column chromatography as a pale yellow powder. Nisamycin is active against Gram-positive bacteria and fungi, and exhibits a cytotoxic activity.
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II. STRUCTURE DETERMINATION AND STRUCTURE-ACTIVITY RELATIONSHIPS
KEN-ICHIRO HAYASHI, MASAHIRA NAKAGAWA, TOMOYUKI FUJITA, SHINJI TANIMOR ...
1994 Volume 47 Issue 10 Pages
1110-1115
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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Nisamycin, a novel manumycin group antibiotic, was isolated from the culture broth of
Streptomyces sp. K106. Structural elucidation of nisamycin was achieved by detailed NMR spectral analyses and comparison of the NMR data of nisamycin with those of other manumycin group antibiotics. The structure was confirmed by chromic acid oxidation. The absolute stereochemistry of nisamycin was determined to be 4
R, 5
S and 6
R from the CD spectra of nisamycin and chromic oxidation of nisamycin. In addition, some structure activity-relationships were examined.
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HANS-PETER FIEDLER, ANDREAS KULIK, TRAUGOTT C. SCHÜZ, CORINNA VOL ...
1994 Volume 47 Issue 10 Pages
1116-1122
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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A new juglomycin-type antibiotic was identified by a HPLC-diode array screening technique in the culture filtrate of
Streptomyces tendae Tü 901/8c. Juglomycin Z (
1) differs from all other known juglomycin compounds by an additional methyl group in position 3 of the naphthoquinone ring system. Juglomycin Z is antibiotically active against Gram-positive and Gram-negative bacteria and against yeasts.
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MASAJI KAWATSU, TAKASHI YAMASHITA, MASAAKI ISHIZUKA, TOMIO TAKEUCHI
1994 Volume 47 Issue 10 Pages
1123-1129
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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The effect of conagenin (CNG) on myelosuppression induced by antitumor agents was investigated. The daily administration of CNG prevented reduction in the number of platelets (PLT) observed in peripheral blood of mice given mitomycin C (10mg/kg) but did not prevent reduction in the number of leukocytes (WBC). The effect on PLT was also confirmed in mice given cyclophosphamide (100mg/kg). In mice given repeated doses of 5-fluorouracil (10mg/kg), CNG prevented the reduction of PLT as well as WBC and maintained them at normal levels. CNG prevented reduction of the production of interleukin-2, 3 and 6 in cultured supernatants of spleen cells taken from mice given 5-fluorouracil.
These results suggest that CNG modulates production of lymphokines which are responsible for thrombopoiesis.
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SETSUKO KUNIMOTO, TOMIO TAKEUCHI
1994 Volume 47 Issue 10 Pages
1130-1135
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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Spergualin (SG) and 15-deoxyspergualin (DSG) are derivatives of spermidine. Their substrate and inhibitor activities toward amine oxidase from bovine plasma were determined. SG was a good substrate of amine oxidase next to spermidine. The
Km and V
max for SG were 46.5 μM and 0.429 μM/minute, respectively, whereas the
Km and V
max for spermidine were 111 μM and 3.75 μM/minute, respectively. Thus SG has about two-fold stronger affinity to amine oxidase than spermidine, but its catalysis rate was one ninth of spermidine. In contrast, DSG was hardly oxidized and inhibited spermidine oxidation at low concentration. Affinity of both compounds for amine oxidase was determined by inhibition kinetics using benzylamine as substrate. SG and DSG competitively inhibited amine oxidase activity showing the
Ki values of 175 μM and 7.46 μM, respectively.
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SHIGENORI KUMAZAWA, MINA KANDA, HIDEYUKI AOYAMA, MASAMI UTAGAWA, JUN K ...
1994 Volume 47 Issue 10 Pages
1136-1144
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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A new peptide antibiotic, aibellin, that had the efficiency enhancing activity on rumen fermentation, was isolated from the culture broth of the fungus,
Verticimonosporium ellipticum D1528, and its primary structure was elucidated from spectrometric analysis and chemical degradation. Aibellin is a 20-residue peptaibol, and it has a unique structural feature in the novel
C-terminal amino alcohol. Moreover, aibellin is the first peptaibol that possesses two acidic amino acids in the
C-terminal region and a Phe residue in the middle of the sequence.
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II. STRUCTURE ELUCIDATION OF AMYTHIAMICIN D
KAZUO SHIMANAKA, YOSHIKAZU TAKAHASHI, HIRONOBU IINUMA, HIROSHI NAGANAW ...
1994 Volume 47 Issue 10 Pages
1145-1152
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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The structure of a unique polythiazole-containing cyclic peptide antibiotic, amythiamicin D, was elucidated by chemical degradations and NMR spectral analyses. Acid hydrolysis of amythiamicin D gave one mole of glycine and three new amino acids. Structures of
N-acetyl-
O-methyl derivatives of these new amino acids were determined by NMR and UV spectral analyses. Connectivities of these amino acids were determined by HMBC experiments.
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III. STRUCTURE ELUCIDATIONS OF AMYTHIAMICINS A, B AND C
KAZUO SHIMANAKA, YOSHIKAZU TAKAHASHI, HIRONOBU IINUMA, HIROSHI NAGANAW ...
1994 Volume 47 Issue 10 Pages
1153-1159
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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The structures of novel antimicrobial antibiotics, amythiamicins A, B and C, were elucidated by chemical degradations and NMR spectral analyses. The main frame from C-1 to C-41 of these antibiotics was the same as that of amythiamicin D. Ammo acid autoanalyses of amythiamicins A, B and C showed that these have another one mole of serine and proline in comparison with amythiamicin D. Stereochemistries of both amino acids were determined to be L by chiral HPLC. These seryl-prolyl residues in amythiamicins A, B and C are attached at C-41 through an oxazoline ring, amide and ester bond, respectively.
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GREGORY M. BRILL, MARIANNA JACKSON, DAVID N. WHITTERN, ALEX M. BUKO, P ...
1994 Volume 47 Issue 10 Pages
1160-1164
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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HIROSHI YOSHIDA, NORIKO ARAI, MASAKO SUGOH, JUN IWABCHI, KAZURO SHIOMI ...
1994 Volume 47 Issue 10 Pages
1165-1166
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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YOSHIO TAKADA, MASAHARU INOUYE, TOSHIRO MOROHOSHI, NAOKI MUTO, FUMIO K ...
1994 Volume 47 Issue 10 Pages
1167-1170
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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SHOZO MIURA, YOSHIYA IWASAKI, KUNIAKI TATSUTA
1994 Volume 47 Issue 10 Pages
1171-1172
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
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KEIICHI MATSUZAKI, HODAKA IKEDA, TOMOAKI OGINO, ATSUKO MATSUMOTO, H. B ...
1994 Volume 47 Issue 10 Pages
1173-1174
Published: October 25, 1994
Released on J-STAGE: April 19, 2006
JOURNAL
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