Sibiromycin, possessing strong antitumor activity in animal experiments, is produced by
Streptosporangium sibiricum. Sibiromycin can be isolated from culture filtrate using ion-exchange or by solvent extraction. The antibiotic was obtained as a crystalline substance with ultraviolet absorption maxima at 230 and 310 nm, [α]
D+525° (in dimethylformamide); the empirical formula-C
24H
31N
3O
7. In 1 N hydrochloric acid, sibiromycin is transformed into "the product of acidic inactivation" (PAI), C
24H
29N
3O
6. Hydrolysis of PAI with 6 NHC1 affords "the product of acidic hydrolysis" (PAH), C
16H
14N
2O
3. Methanolysis of sibiromycin and PAI yields the methylglycoside of a new amino sugar sibirosamine, C
8H
16NO
3 (OCH
3). Aqueous alkaline hydrolysis of PAI and PAH affords the crystalline substance C
8H
9NO.
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