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Volume 34, Issue 3
Displaying 1-18 of 18 articles from this issue
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IWAO UMEZAWA, HIDEO TAKESHIMA, KANKI KOMIYAMA, YOSHIE KOH, HIROSHI YAM ...1981 Volume 34 Issue 3 Pages 259-265
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSA new antibiotic, stubomycin, was isolated from the culture broth and mycelia of Streptomyces strain No. KG-2245. Stubomycin was prepared as colorless plates and has the empirical formula C29H35NO5. The antibiotic possesses growth inhibitory activity against Gram-positive bacteria and transplantable murine tumors, such as Ehrlich carcinoma, and leukemia P388. The antibiotic also shows direct cytotoxic activity against HeLa cells in vitro.View full abstractDownload PDF (3053K) -
1. TAXONOMY, FERMENTATION, ISOLATION AND BIOLOGICAL ACTIVITIESHIROFUMI NAKANO, YUZURU MATSUDA, KUNIO ITO, SHUJI OHKUBO, MAKOTO MORIM ...1981 Volume 34 Issue 3 Pages 266-270
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSGilvocarcin V and gilvocarcin M, a group of antitumor antibiotics with a novel skeleton, were discovered in culture broths of Actinomycete DO-38. The producing organism, subsequently determined to be a new species and named Streptomyces gilvotanareus (NRRL 11382). Gilvocarcin V and M were isolated by ethyl acetate extraction and chromatography on silica gel. The antibiotics are active against Gram-positive bacteria and experimental tumors such as mouse sarcoma 180 and mouse leukemia P388.View full abstractDownload PDF (2493K) -
2. STRUCTURAL ELUCIDATIONKEIICHI TAKAHASHI, MAYUMI YOSHIDA, FUSAO TOMITA, KUNIKATSU SHIRAHATA1981 Volume 34 Issue 3 Pages 271-275
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSGilvocarcin V(1), C27H26O9, m.p. 264-267°C (dec.), and gilvocarcin M(2), C26H26O9, m.p. 245-248°C (dec.), are new antitumor antibiotics produced by Streptomyces gilvotanareus. The structure of gilvocarcins has been determined by chemical degradation, nmr and mass spectra. They have a benzonaphtopyran-one system, to which the furanose moiety is linked through a C-C glycosyl bond.View full abstractDownload PDF (2291K) -
STRUCTURE OF MYCINAMICIN IIIMITSUO HAYASHI, MASARU OHNO, SEIJI KATSUMATA, SHUZO SATOI1981 Volume 34 Issue 3 Pages 276-281
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSThe structure of mycinarnicin III coproduced with other mycinamicins in submerged fermentation of Micromonospora griseorubida sp. nov. has been elucidated by its spectral properties and chemical degradation studies. Mycinamicin III is 3″-O-demethyl mycinamicin IV.View full abstractDownload PDF (2117K) -
JANUSZ W. KRAJEWSKI, ZOFIA URBANCZYK-LIPKOWSKA, PRZEMYSLAW GLUZINSKI1981 Volume 34 Issue 3 Pages 282-287
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSA new type of 6-formamidinepenicillanic acid in which the ω-nitrogen atom is involved in the azaheptane ring (mecillinam) having a strong selective activity against Gram-negative bacteria strains has been investigated by the X-ray single-crystal diffraction methods using crystals in the solvated state. The conformation of penam part as well as of the amidine group is discussed. Two independent molecules of mecillinam found in the asymmetric unit of the crystal cell differ from each other in their detailed conformations. The problem of the stability of the compound has been discussed also.View full abstractDownload PDF (2918K) -
KIKUO IGARASHI, TSUNETOSHI HONMA, TAKASHI FUJIWARA, EIJI KONDO1981 Volume 34 Issue 3 Pages 288-291
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSSynthesis of (1D)-1, 3, 5/2, 4- and 1L)-1, 2, 4/3, 5-5-aminocyclohexanetetrols starting from kanamycin A is described.View full abstractDownload PDF (1589K) -
WEN-CHIH LIU, G. L. ASTLE, J. S. WELLS, L. R. CRUTHERS, T. B. PLATT, W ...1981 Volume 34 Issue 3 Pages 292-297
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSA method is described for isolation of grail quantities of the components of the streptothricin complex S15-1 utilizing CM Sephadex column chromatography eluted with 10% acetic acid as an eluant followed by gradient elution with 10% acetic acid containing 0.02N-0.03 N HCl. Streptothricins F and E, as well as an unidentified component C1, have been isolated and their comparative biological activities determined. Streptothricins F and E were comparable in taeniacidal activity in mice infected with Hymenolepis nana as feeding either one at 0.05% in the diet removed 92-100% of the adult tapeworms. The unidentified component C1 was inactive at the levels tested. In contrast, component C1 was the most active in antimicrobial activity against Bacillus subtilis and in inhibiting the urcase activity of Proteus mirahilis. In the former test, the ratios of activity were; 1 : 7: 30 for F: E : C1 and in the latter; 1 : 2: 4 for F: E: C1.View full abstractDownload PDF (2975K) -
THE BIOLOGICAL ACTIVITY OF SICCAYNE, ISOLATED FROM THE MARINE FUNGUS HALOCYPHINA VILLOSA J. & E. KOHLMEYERJUTTA KUPKA, TIMM ANKE, WOLFGANG STEGLICH, LOTHAR ZECHLIN1981 Volume 34 Issue 3 Pages 298-304
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSFrom submerged cultures of the marine basidiomycete Halocyphina villosa we isolated siccayne (4-(2, 4-dihydroxyphenyl)-2-methyl-l-buten-3-yne) (1), a metabolite first described from fermentations of the deuteromycete Helminthosporium siccans. Siccayne is a moderately active antibiotic, which inhibits Gram-positive bacteria and some fungi at concentrations of 10-50μg/ml. Its cytotoxic effect is much more pronounced on both normal and Roussarcoma-virus transformed chicken embryo fibroblasts as compared to cells of the Ehrlich ascites carcinoma. Siccayne apparently interferes with the uptake of nucleoside precursors into eucaryotic cells as well as with the in vitro incorporation of nucleotides into DNA and RNA.View full abstractDownload PDF (3311K) -
MASAMI TSUCHIYA, KAYOKO SUZUKAKE, MAKOTO HORI, TSUTOMU SAWA, TOMIO TAK ...1981 Volume 34 Issue 3 Pages 305-312
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSThe macrolide-resistance of multiple-drug resistant strains of Staphylococcus aureus was divided into two types; the decreased sensitivity of ribosomes (type I) and the decreased uptake (type II). Both types were resistant to erythromycin, tylosin and 3-acetyltylosin, and their resistance was not inducible. 3-Acetyl-4″-isovaleryltylosin inhibited the growth of both types. Protein synthesis on ribosomes of type I in vitro (S. aureus MS-9610) was inhibited by 3-acetyl-4″-isovaleryltylosin, but little or no inhibition was seen with either tylosin or 3-acetyltylosin. Ribosomes of type II in vitro (S. aureus MS-8710) were sensitive to all macrolides. 3-Acetyl-4"-isovaleryltylosin accumulated about twice as much as 3-acetyltylosin in intact cells of type II.View full abstractDownload PDF (4111K) -
FUMIAKI TAGUCHI, YOSHIKO IMATANI, DAIZO NAGAKI, AKIRA NAKAGAWA, SATOSH ...1981 Volume 34 Issue 3 Pages 313-316
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSThe effect of new anti-mycoplasmal antibiotic, 2'-amino-2'-deoxy-9-β-D-ribofuranosyl adenine (2-AA) on virus multiplication was investigated. The 2-AA inhibited only the multiplication of measles virus among the viruses tested; i.e. , herpes simplex virus, BK virus, vesicular stomatitis virus, measles virus and Echo virus. At a concentration of 5μg/ml of 2-AA, the inhibition of measles virus replication was complete, i.e., no infectious virus nor viral antigen detected. In contrast, 9-β-D-arabinofuranosyl adenine (50μg/ml) was active to herpes simplex virus and BK virus, and was inactive to measles virus, vesicular stomatitis virus and Echo virus. Results described herein may suggest that 2-AA affects the late function (perhaps the translation step) of the replication of measles virus.View full abstractDownload PDF (2107K) -
KAZUMITSU UEDA, JUNJI MORITA, TOHRU KOMANO1981 Volume 34 Issue 3 Pages 317-322
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSThe action of mitomycin C on double-stranded replicative form I DNA (RF I DNA; supercoiled, covalently closed, circular duplex DNA) of bacteriophage φX174 was investigated using the technique of agarose gel electrophoresis. Mitomycin C reduced with sodium hydrosulfite (sodium dithionite, Na2S2O4) caused single strand scission in φX174 RF I DNA in the presence of Cu2+. Cu2+ was essential for this DNA cleavage action, and other transition metal ions such as Fe2+, Fe3+, Mn2+, Co2+ and Zn2+ were of no effect. This DNA strand scission was inhibited by catalase (EC 1.11.1.6) and various radical scavengers. This DNA strand scission was caused by free oxygen radicals generated during autoxidation of reduced mitomycin C in the presence of Cu2+.View full abstractDownload PDF (3019K) -
MOHAMED A. MARAHIEL, RUDOLF LURZ, HORST KLEINKAUF1981 Volume 34 Issue 3 Pages 323-330
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSA covalently closed circular (ccc) DNA, with a weight of 44.7×106 daltons, has been isolated from Bacillus brevis ATCC 9999 (a gramicidin S producer) and from the gramicidin S-negative mutant EB16. The ccc DNA in the case of the parent strain, is mainly (99%) attached to the chromosomal and membrane fraction. A restriction enzyme map of the plasmid DNA was constructed for the enzymes SalI, SmaI and BamHI, which cleaved the plasmid DNA into two, two and six fragments respectively. Further digestion with the endonucleases EcoRI and HindIII cleaved the plasmid into 17 and 22 fragments.View full abstractDownload PDF (4037K) -
MASAAKI ISHIZUKA, TOMIO TAKEUCHI, TORU MASUDA, SHIGEKI FUKASAWA, HAMAO ...1981 Volume 34 Issue 3 Pages 331-340
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSAntitumor antibiotics were examined as possible candidates that possess activity which inhibits preferentially suppressor cells in comparison with effector cells. In screening for such compounds among known antibiotics, aclacinomycin was found to augment antibody formation and delayed-type hypersensitivity in mice over a wide concentration range. The addition of aclacinomycin to mouse spleen cell cultures also enhanced antibody formation in vitro. The generation of suppressor cells or the suppressor activity per se in mice immunized with high doses of SRBC was reduced by aclacinomycin. These results suggest that the drug may possibly inhibit suppressor cells selectively. The administration of aclacinomycin at low doses exhibited antitumor effects on IMC carcinoma; the effect was not dose-dependent.View full abstractDownload PDF (4932K) -
DAVID ROSI, MARION L. DROZD, MICHAEL F. KUHRT, LOUIS TERMINIELLO, PAUL ...1981 Volume 34 Issue 3 Pages 341-343
Published: 1981
Released on J-STAGE: April 12, 2006
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YOSHIRO OKAMI, MASACHIKA TAKASHIO, HAMAO UMEZAWA1981 Volume 34 Issue 3 Pages 344-345
Published: 1981
Released on J-STAGE: April 12, 2006
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ISOLATION AND STRUCTURES OF MYCINAMICIN AGLYCONES, MYCINOLIDE IV AND VMITSUO HAYASHI, MASARU OHNO, KENJI KINOSHITA, SHUZO SATOI, MAKOTO SUZU ...1981 Volume 34 Issue 3 Pages 346-349
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSDownload PDF (1368K) -
STEPHEN HANESSIAN, RENE ROY, MICHEL THERIEN, DANIEL DELORME1981 Volume 34 Issue 3 Pages 350-352
Published: 1981
Released on J-STAGE: April 12, 2006
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PREPARATION OF DEGLYCOBLEOMYCIN BY MILD ACID HYDROLYSIS OF BLEOMYCINYASUHIKO MURAOKA, MASANOBU SUZUKI, AKIO FUJII, YOJI UMEZAWA, HIROSHI N ...1981 Volume 34 Issue 3 Pages 353-357
Published: 1981
Released on J-STAGE: April 12, 2006
JOURNAL FREE ACCESSDownload PDF (2156K)
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