巻号一覧
42 巻 (1990)
- 6 号 p. 683-
- 5 号 p. 575-
- 4 号 p. 467-
- 3 号 p. 289-
- 2 号 p. 171-
- 1 号 p. 1-
42 巻, 3 号
選択された号の論文の5件中1～5を表示しています
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乳酸の胎盤輸送機構を中心として南渕 芳1990 年 42 巻 3 号 p. 289-298
発行日: 1990年
公開日: 2011/07/05
ジャーナル フリー1. To elucidate the human placental L-lactate transport mechanism, we investigated L-lactate uptake by microvillous membrane vesicles (MMV) prepared from human placenta using the rapid filtration technique.
1) The transport of L-lactate into the MMV was dependent on the H+ gradient (extravesicular>intravesicular) and this H+ dependent L-lactate uptake showed a tendency to overshoot. This overshoot disappeared with the addition of H+ ionophore (FCCP). This transport activity increased with gestational age. The transport activity of full-term placenta was 1.65 times more than that of early-term placenta.
2) The initial rate of this H+ dependent L-lactate transport into the MMV exhibited saturation kinetics with respect to the L-lactate concentration : an apparent Km of 4.35 mM and Vmax of 2.78 nmol/mg protein/20 sec were calculated.
These results indicated that there existed an H+ and L-lactate co-transport system in human placental MMV.
2. We studied the placental transport of L-lactate to the fetus with pregnant rats.
1) L-lactate was transported from the mother to the fetus via the placenta without being metabolized.
2) L-lactate was after placental transport, distributed to the fetal organs, (fetal heart, Kidney, brain and lungs etc.) L-lactate uptake to the heart was maximum, and 9.1 times more than that to the muscle.
By these results, we clarified the placental transport system of L-lactate with pregnant rats.
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巽 利昭1990 年 42 巻 3 号 p. 299-307
発行日: 1990年
公開日: 2011/07/05
ジャーナル フリーSperm immobilizing antibodies (SI-Abs) are found in some sera of unexplained cases of sterile women and the correlation between SI-Abs and sterility has been well established. However, the relationship between antibody production and sterility has not been clarified yet. In this study, in order to analyze the mechanism of sterility by SI-Abs, immunoglobulin (Ig) classes and subclasses of SI-Abs found in sterile women were investigated.
For determination of Ig classes and subclasses, SI-Abs positive sera were sequentially absorbed with Staphylococcus aureus (Cowan I strain) and Sepharose beads which bound highly subclass specific anti-human Ig antibodies. The results showed that only two had IgM dominant SI-Abs and the others had IgG dominant SI-Abs among 14 patients tested. Eight patients who had IgG dominant SI-Abs were further studied for IgG subclasses. Six of them belonged to IgG 1, one to IgG 2 and another one equally to IgG 1, IgG 2 and IgG 3. Interestingly, SI-Ab activities increased in most cases after removing IgG 4 subclass from their sera with immunoadsorbent beads. These results suggested that IgG class was dominantly produced in SI-Ab in the majority of patients and IgG 4 subclass might have an effect in neutralizing SI-Ab activities of other IgG subclasses in patient's sera. This speculation was confirmed by the following result that SI-Ab activity in patient's sera was significantly diminished by adding an affinity-purified IgG 4 isolated from the same patient's sera, when SI-Ab activity was assayed by the quantitative sperm immobilizing test. The latter finding will give a hope to approaching a new therapy for immunological sterility.抄録全体を表示PDF形式でダウンロード (1590K) -
石崎 浩之1990 年 42 巻 3 号 p. 309-316
発行日: 1990年
公開日: 2011/07/05
ジャーナル フリーHuman monoclonal antibody (Mab H 6-3 C 4) with strong sperm immobilizing and agglutinating activities was generated by cell fusion of human lymphocytes from a sterile woman with sperm immobilizing antibody and mouse myeloma cells and was used for immunohistochemical studies for the corresponding antigen in male genital organs. This Mab was also used for electron microscopic studies on morphological changes of spermatozoa after treatment with Mab, Immunohistochemical studies using the ABC staining system showed that the antigen epitope corresponding to MabH 6- 3 C 4 was mainly localized in epithelial layers of cauda epididymidis and seminal vesicle but not in testis, caput and corpus epididymides, or prostate. Electron microscopic observation of the positively stained cauda epididymidis showed that the antigen was densely concentrated in stereocilia of the principal cells of the epithelium. The antigen was also observed on spermatozoa in the luminal duct of cauda epididymidis and ejaculated spermatozoa. When ejaculated spermatozoa were incubated with biotinylated Mab H 6-3 C 4 and followed by the reaction with ferritin conjugated avidin for immuno electron microscopy, ferritin particles were observed on entire surfaces of sperm plasma mambrane. When ejaculated spermatozoa were incubated with Mab H 6-3 C 4 and guinea pig serum as complement, remarkable morphological changes such as peeling-out of plasma membrane or formation of numerous holes on plasma membrane were observed by scanning and transmission electron microscope.抄録全体を表示PDF形式でダウンロード (9653K) -
石川 敬次1990 年 42 巻 3 号 p. 317-326
発行日: 1990年
公開日: 2011/07/05
ジャーナル フリーSixty-three patients whose chief complaint was hypermenorrhea were examined and their F VIII : C, F VIII R : AG, and F VIII R : WF (vWF) measured to determine the relationship between the disorder and the blood coagulation factors. The result of the measurements indicated that the examined patients could be divided into two groups; one of young women of twenty years old or thereabout suffering from an abnormal duration of menstrual bleeding, and one of women of early middle age around forty years old suffering from an abnormally heavy menstrual flow. The majority of both groups were seen to show abbormal coagulation factor values, and furthermore, it was discovered that by repeating the measurement of the coaulation factors more than twice, the values fluctuated continuously. It is, therfore, considered likely that the previously-measured abnormally high value could shift to a value below normal. Nor is it likely that an abnormally low value is invariably accompanied by an extended period of menstrual blood factors such as vascular endothelial cells secreting R : AG and vWF, endometrial immunity plays an important part in triggering abnormal menstrual bleeding.抄録全体を表示PDF形式でダウンロード (1493K) -
1990 年 42 巻 3 号 p. 341-408
発行日: 1990年
公開日: 2011/07/05
ジャーナル フリーPDF形式でダウンロード (34076K)
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