巻号一覧
46 巻 (1994)
- 6 号 p. 689-
- 5 号 p. 559-
- 4 号 p. 379-
- 3 号 p. 283-
- 2 号 p. 165-
- 1 号 p. 1-
46 巻, 5 号
選択された号の論文の6件中1～6を表示しています
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茨木 保, 斎藤 滋, 一條 元彦1994 年 46 巻 5 号 p. 559-568
発行日: 1994/09/01
公開日: 2011/07/05
ジャーナル フリーBy the polymerase chain reaction (PCR), we have developed a system for type-specific (human papilloma virus (HPV) -16, 18 and 33) as well as universal detection of HPV. We applied this system to 120 uterine cervical tissue specimens, and detected HPV DNA in 84% (41/49) of the carcinomas, 61% (20/33) of the CIN and 3% (1/38) of the normal cervical tissues. To clarify the association between p53 mutation and HPV infection, the presence of p53 mutation was examined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). By this assay, we detected no mutations in the p53 gene in 14 cervical carcinomas (9 HPV-positive, 5 HPV-negative). This suggests that inactivation of p53 by point mutation is infrequent in cervical carcinomas irrespective of the presence or absence of HPV infection.
[Adv Obstet Gynecol 46 (5); 559-568, 1994 (H6.9)]抄録全体を表示PDF形式でダウンロード (6514K) -
浦川 信司, 安田 勝彦, 堀越 順彦, 椹木 勇1994 年 46 巻 5 号 p. 569-578
発行日: 1994/09/01
公開日: 2011/07/05
ジャーナル フリーProstaglandins (PGs) play an important role in pregnancy, labor and delivery, especially in the onset and progress of labor. We measured and identified PGs and their metabolites in the urine during pregnancy, labor, and the puerperium. PGs and their metabolites were measured using derivatization with anthryldiazomethane (ADAM) and high performance liquid chromatography (HPLC).
Urine samples were collected from pregnant women at various stage of gestation, postpartum women (1, 3 and 5 days after delivery), three nonpregnant women and two men.
PGs and their metabolites were extracted from urine with octadecylsilyl silica by the method of Powell and re-extracted to remove substances that interfered with derivatization using ADAM. The ADAM derivatives of PGs and their metabolites were applied to reverse phase HPLC under condition A (solvent system, methanol : water 75 : 25 (v/v); flow rate, 1ml/min) and condition B (solvent system, acetonitrile : water : phosphoric acid 60 : 39.9 : 0.1 (v/v/v); flow rate, 1ml/min) and detected using a fluorescence spectrophotometer (EX 365 nm, EM 412 nm).
Specific chromatographic peaks were observed in urine samples obtained from the pregnant women after more than five weeks of gestation, but were not observed in the samples obtained from nonpregnant women and men. In addition, the specific urinary peaks in the pregnant women disappeared within five days after delivery. The retention times of the specific chromatographic peaks were 54 min, 58 min, 62 min, 67 min, 71 min under condition A, while the times were 37 min, 39 min, 43 min, 57 min, and 61 min under condition B.
PGE2, PGF2α, 15-keto-PGE2, 15-keto-PGF2α, 13, 14-dihydro-15-keto-PGE2, 13, 14-dihydro-15-keto-PGF2α, metabolites of estrogen and progesterone, malonic acid, oxalic acid, and hippuric acid were also studied using delivertization with ADAM and HPLC. However, none of the peaks of these substances were consistent with the specific peaks in the urine of pregnant women. These findings suggest that the urinary peaks obtained in our pregnant women were pregnancy-specific. However, identification of the substances that produce these pregnancy-specific peaks has not yet been achieved and further studies are needed for this purpose. [Adv Obstet Gynecol 46 (5); 569-578, 1994 (H6. 9)]抄録全体を表示PDF形式でダウンロード (1299K) -
スリ・カダルシ ソヨノ, 森川 肇, 望月 眞人1994 年 46 巻 5 号 p. 579-587
発行日: 1994/09/01
公開日: 2011/07/05
ジャーナル フリーインドネシアの風土病としての甲状腺腫多発地域 (Ponjong地域) における母体の甲状腺機能とその胎児発育への影響を明らかにするために, 以下の検討を行った.甲状腺腫多発地域 (EG地域) と甲状腺腫の認められない地域 (NEG地域) における非妊婦30名および妊娠末期の妊婦30名をそれぞれ対象として, 妊婦の甲状腺機能としてはfree T3, free T4, TSHを, また栄養代謝物質動態としては空腹時血糖, 血中遊離脂肪酸濃度, 血中総蛋白量を測定した.また, 両地域の妊婦については分娩時の新生児体重を測定した. EG地域における非妊婦人の血中freeT4濃度はNEG地域非妊婦人に比べ有意に低下していた.一方, EG地域においては, 妊娠末期の妊婦血中freeT4濃度は非妊婦に比べ低下していたが, freeT3とTSH濃度には有意差を認めなかった.また, NEG地域においては, 妊娠末期の妊婦血中freeT3とfreeT4濃度は非妊婦に比べ低下していたが, TSH濃度には有意差を認めなかった.さらに, 妊婦甲状腺機能の比較検討では, EG地域妊婦の血中freeT3とfreeT4濃度はNEG地域妊婦のそれらに比べ有意に高値であった.血中栄養代謝物質を検討したところ, 血中遊離脂肪酸濃度と血中総蛋白量については両地域の妊婦の間に有意差を認めなかったが, 母体空腹時血糖値と新生児体重はEG地域においてNEG地域に比べ有意に高値であった.これらの成績から, EG地域においてはNEG地域に比べて相対的な母体の甲状腺機能亢進状態が存在し, これが母体代謝への作用を介して相対的な高血糖を惹起する結果, 胎児発育が促進されたものと推察された. [産婦の進歩46 (5);579-587, 1994 (平成6年9月)]抄録全体を表示PDF形式でダウンロード (1267K) -
窪田 耕三, 津本 美也子, 岡田 直樹, 鹿戸 陽一, 柴田 政彦, 中田 弓子, 多田 仁彦1994 年 46 巻 5 号 p. 588-593
発行日: 1994/09/01
公開日: 2011/07/05
ジャーナル フリーWe evaluated the effect on postoperative pain relief and the incidence of side effects of continuous epidural infusion of buprenorphine 0.2mg (n=25) or 0.4mg/day (n=70), intramuscular injection of 0.2mg b.i.d. (n=12) and other analgesics administered p. r. n. for pain (n=17) for a period of 48 hrs after abdominal simple hysterectomy.
Pain relief was estimated by each patient on a pain score of 0 to 10 (0 : no pain ; 10 : severe pain). Continuous epidural infusion of buprenorphine at the speed of 0.4mg/day after a bolus injection of 0.1mg proved to be most efficacious in giving a stable pain relief and was associated with nausea and/or vomiting in 42.9 % of cases, while 50.0 % of the intramuscular injection group and 17.6 % of the other analgesics group had nausea and/or vomiting. The high incidence (42.9 %) of nausea and/or vomiting in the 0.4mg epidural group declined to 23.0 % after the prophylactic use of Domperidone suppositories, but additional management is necessary to obtain a satisfactory pain relief.
[Adv Obstet Gynecol 46(5) 588-593, 1994 (H6. 9)]抄録全体を表示PDF形式でダウンロード (944K) -
1994 年 46 巻 5 号 p. 625-644
発行日: 1994/09/01
公開日: 2011/07/05
ジャーナル フリーPDF形式でダウンロード (4656K) -
1994 年 46 巻 5 号 p. 645-662
発行日: 1994/09/01
公開日: 2011/07/05
ジャーナル フリーPDF形式でダウンロード (6435K)
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