Eleven organs of the rat were studied 1 hr and 24 hr after the intravenous administration of
67CuCl
2 and
67Cu-eeruloplasmin. The rats were normal, copper-deficient, or copper-laden. The amounts of stable copper and
67Cu in the whole organ and supernatant fractions, corrected for whole blood copper and
67Cu, were measured. The distribution of supernatant
67Cu was determined in three Sephadex G-100 chromatographic zones: Peak I (150, 000 daltons), Peak II (31, 000 daltons), and Peak III (11, 000 daltons). All organs took up
67Cu from both sources, but there was a tendency for increased uptakes in copper-deficient rats and decreased uptake in copper-laden rats. Only lung, heart, and testis took up more
67Cu from
67Cu-ceruloplasmin than from 87CuCl
2. Supernatant
67Cu tended to be in Peak I when the source was
67Cu-ceruloplasmin and in Peak 11 when the source was
67CuCl
2.. When
67Cu-ceruloplasmin was added to supernatant fractions in vitro, the
67Cu was in Peak I. However, when
67Cu Cl
2 was added to supernatant frac-tions, Peak III predominated in kidney, brain, testis, and liver; Peak II pre-dominated in none; and Peak I predominated in spleen, muscle, large and small bowel, stomach, lung, and heart. A high-molecular-weight copperbinding substance seems to be present in organ supernatant fractions.
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