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16 巻
選択された号の論文の657件中401～450を表示しています
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上久保原稿種別: 本文
1959 年 16 巻 p. 597-
発行日: 1959年
公開日: 2017/12/22
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上久保原稿種別: 本文
1959 年 16 巻 p. 597-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 597-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 597-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 598-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 598-599
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 599-600
発行日: 1959年
公開日: 2017/12/22
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高田原稿種別: 本文
1959 年 16 巻 p. 600-
発行日: 1959年
公開日: 2017/12/22
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上久保原稿種別: 本文
1959 年 16 巻 p. 600-
発行日: 1959年
公開日: 2017/12/22
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茂木 劼原稿種別: 本文
1959 年 16 巻 p. 601-616
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーResearches were made on serial and individual fluctuations of the riboflavin metabolism in healthy body by measuring the total riboflavine and its bound form in blood, and the excretion of riboflavine in urine also. Furthermore, the correlation between nicotinic acid and riboflavin metabolism has been studied. The computed average total riboflavin level in blood (12 men and 11 women) is 8.0±1.46 μg/dl, fluctuation of which is rather small both individually and serially through an year. An average level in blood of the bound form of riboflavin (10 men and 11 women) is 6.5±1.11 μg/dl, fluctuation of which is small individually or serially, but through an year some fluctuations are noticed. An average excretion of riboflavin in urine per day (11 men and 7 women) is 1382±603 μg, and its serial fluctuation is comparatively small, while individual or serial fluctuation through an year is found to be remarkable. A slight correlation is noticed between the total riboflavin level in blood and the amount of urinary excretion of nicotinic acid and its metabolized products.抄録全体を表示PDF形式でダウンロード (1071K) -
西岡 延一郎原稿種別: 本文
1959 年 16 巻 p. 616-621
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe nutritional requirement of Lactobacillus brevis was studied and the following result was obtained. The bacillus requires for its growth, thiamine, biotin, folic acid, nicotinic acid, pantothenic acid, uracil, cytosine and orotic acid. The bacillus may be used for the bioassay of uracil, cytosine or orotic acid. The range of assay may be 1-10 μg per ml. The inhibitory effect of thiouracil was investigated and it was confirmed that the bacillus has not the synthetic ability of aspartic acid, uredosuccinic acid or dihydroorotic acid.抄録全体を表示PDF形式でダウンロード (587K) -
高田原稿種別: 本文
1959 年 16 巻 p. 621-
発行日: 1959年
公開日: 2017/12/22
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西岡 延一郎原稿種別: 本文
1959 年 16 巻 p. 622-627
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーIn previous paper, it was confirmed that Lactobacillus brevis required for its growth, uracil, cytosine or their nucleoside and nucleotide and orotic acid. Further examination was made with the growth effect of RNA, DNA, apuric acid and deamino-DNA. It was confirmed that these polymers were not effective while chemical or enzymatical hydrolysates of these compounds were effective.抄録全体を表示PDF形式でダウンロード (633K) -
高橋 郁夫原稿種別: 本文
1959 年 16 巻 p. 627-633
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe present paper deals with the investigation attempted to demonstrate a precursor of riboflavin in the culture of Lactobacillus plantarum using the inhibitors. Lactobacillus plantarum was grown in bouillon or wort and 3 types of riboflavin was determined in bacterial cell and broth in the presence of various antienzymic substances, and tried to discover a precursor of riboflavin formation by interfering with PAS or sulfamine. The total riboflavin content of growing bacillus was higher in bouillon culture than in wort. The amount of riboflavin in bacterial cell was higher than in the broth. Bacterial cell contained high FAD, less FMN and no free riboflavin. On the other hand, in the broth free riboflavin was the highest, FMN was slight and none of FAD. Fluorescent substances were found having Rf 0.14 in the cell and Rf 0.49 in the broth. KCN, NaF, PAS or CH_2ICOOH inhibited the riboflavin formation, while an increase was observed by sulfamine and malonate. Non-acetylatable diazotizable amine which was supposed to be a precursor of riboflavin synthesis was accumulated in the presence of PAS or sulfamine.抄録全体を表示PDF形式でダウンロード (844K) -
小竹 淳一原稿種別: 本文
1959 年 16 巻 p. 634-638
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーAfter tryptophan was administrated orally to riboflavin-deficient rats or to normal rats three types of riboflavin (free form, FMN and FAD) were injected subcutaneusly respectively and the urinary excretion of anthranilic acid, xanthurenic acid, N-methyl nicotinamide and kynurenine were investigated. In normal rats any appreciable difference was hardly found. While, in riboflavin-deficient rats that were fed tryptophan only, a considerable amount of anthranilic acid, xanthurenic acid, and of small amount of N-methylnicotinamide were observed in the urine, while kynurenine was not changed. When tryptophan and riboflavin were administrated, the following results were obtained. Urinary anthranilic acid was not changed by the injection of free type of riboflavin, decreased by FAD, considerably decreased by FMN. A decrease was observed in xanthurenic acid excretion with free riboflavin and FAD, while FMN was unchanged. N-methylnicotinamide was increased by free riboflavin and FAD, and remarkably increased by FMN. Kynurenine was not changed.抄録全体を表示PDF形式でダウンロード (508K) -
田村 松三原稿種別: 本文
1959 年 16 巻 p. 639-646
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーMeasurement was made on the riboflavin content and the percentage of 3 forms of riboflavin in the organs of rats which were suffered from diabetes by administration of tryptophan-sodium lactate mixture, pyridoxal-deficient diet and alloxan injection. In the tryptophan-sodium lactate administrated rats, riboflavin content was decreased in liver, kidney and brain considerably, in heart slightly, and was unchanged in pancreas and muscle. In the pyridoxal-deficient rats, a decrease was observed in riboflavin content in liver, kidney, heart, pancreas, brain and muscle, marked in liver, kidney and heart. In the alloxan diabetic rats, riboflavin content was decreased remarkably in liver, kidney, heart, pancreas and muscle, but it was not decreased in brain. No considerble change was observed in the percentage of 3 forms of riboflavin in the organs of those rats.抄録全体を表示PDF形式でダウンロード (755K) -
池田 仁紀原稿種別: 本文
1959 年 16 巻 p. 646-651
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーMethods were described for the extraction of the intracellular thiaminase by use of dry-ice. Two kinds of enzyme thus obtained from Bacillus thiaminolyticus and Clostridium thiaminolyticum, respectively were characterized based on the rate of migration in an electric field. It was of interest that the extracted thiaminase from B.thiaminolyticus had stronger activity on thiamine than that from Cl.thiaminolyticum while the latter was found much purer than the former.抄録全体を表示PDF形式でダウンロード (656K) -
清水原稿種別: 本文
1959 年 16 巻 p. 651-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 651-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 651-
発行日: 1959年
公開日: 2017/12/22
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前田 義徳原稿種別: 本文
1959 年 16 巻 p. 652-657
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーRabbits were immunized with 4 strains of Clostridium thiaminolyticum. The examined Bacillus, vegetative form, is not sporelized. With these immune sera of animals the agglutination and precipitation were undertaken against Cl.thiaminolyticum, Bacillus aneurinolyticus, Bacillus thiaminolyticus and Clostridium histoliticum H-22. Spontaneous agglutination was removed by a new method. As the results of these examinations, it was evidenced that immune serum has the specific agglutinin of its own, shows ×10240〜×1280 agglutinin titer with the own agglutinogen, but does not react with other strains. Precipitation shows principally the same results as the agglutination. The precipitation and agglutination of the immune sera against Clostridium histoliticum H-22,B.aneurinolyticus and B.thiaminolyticus proved negative.抄録全体を表示PDF形式でダウンロード (556K) -
前田 義徳原稿種別: 本文
1959 年 16 巻 p. 657-660
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーRabbits were immunized by the culture media (V・F-bouillon) of Clostridium thiaminoliticum. Precipitation was proved between the immune sera and the culture fluid. The utmost titer of the precipitation shows 2048 time dilution of the serum, when the homologous antigen was used.抄録全体を表示PDF形式でダウンロード (432K) -
前田 義徳原稿種別: 本文
1959 年 16 巻 p. 661-663
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーI have proved the existence of the thiaminase, in the supernatant of the precipitation test, by which the Cl.thiaminolyticum culture and its immune serum was mixed. By the ring test the disapperance of the thiaminase was observed according to the time lapse and the serum volume. In the mixed method, 73〜84% thiaminase disappeared after 2 hours. Thus the author have demonstrated the existence of anti-thiaminase in the immune sera.抄録全体を表示PDF形式でダウンロード (338K) -
前田 義徳原稿種別: 本文
1959 年 16 巻 p. 663-664
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーInfluence of thiaminase activity was examined by the addition of immune serum against Clostridium thiaminoliticum culture. The experiment was carried on with the most suitable condition, i.e.pH 7.5,37℃, 30 minutes. The result shows by the Yoshikawa strain 57〜67% and by the Tomikawa strain 25〜45% decrease of the activity. This is also the evidence that anti-thiaminase exists in the immune serum.抄録全体を表示PDF形式でダウンロード (264K) -
熨斗 嘉文原稿種別: 本文
1959 年 16 巻 p. 665-667
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe results obtained indicated that rabbits, following injection of the crude suspensions of thiaminase I or II, developed precipitin production in the serum. Thus, precipitation reaction revealed the specific difference between thiaminase I and II.抄録全体を表示PDF形式でダウンロード (360K) -
上久保原稿種別: 本文
1959 年 16 巻 p. 667-
発行日: 1959年
公開日: 2017/12/22
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熨斗 嘉文原稿種別: 本文
1959 年 16 巻 p. 668-670
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe rabbit antiserum immunized with bacterial thiaminase inhibited the thiamine decomposing activity of the crude suspension of thiaminase. The inhibitory effect on thiaminase I or II was shown to be a specific reaction. The crude suspension of thiaminase from which the precipitate produced in precipitation reaction were removed lost its thiamine decomposing activity.抄録全体を表示PDF形式でダウンロード (368K) -
熨斗 嘉文原稿種別: 本文
1959 年 16 巻 p. 670-672
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーOne hundred and twenty-eight of 528 human sera reacted positively with the crude suspension of thiaminase in precipitation reaction. Of these positive 128 sera, 82 sera reacted with thiaminase I, 31 sera with thiaminase II, and 15 sera with the both enzymes. Fourteen strains of Bacillus thiaminolyticus were isolated from the feces of 41 cases whose sera were positive in precipitation reaction with thiaminase I, and 4 strains of Bacillus aneunolyticus from the 14 positive cases with thiaminase II. The human sera that were positive in precipitation reaction inhibited the thiamine decomposing activity of thiaminase.抄録全体を表示PDF形式でダウンロード (349K) -
熨斗 嘉文原稿種別: 本文
1959 年 16 巻 p. 672-675
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーIt was demonstrated that chickens fed with thiamine-decomposing bacteria may produce specific precipitin in sera to thiaminase I and II respectively. It was not always successful at all times to isolate the thiamine-decomposing bacteria from the feces of the human beings who have these bacteria in their intestines. These bacteria may appear at intervals of several days in feces. Therefore, a single attempt of isolation could not decide the positive rate of the carriers.抄録全体を表示PDF形式でダウンロード (424K) -
上久保原稿種別: 本文
1959 年 16 巻 p. 675-
発行日: 1959年
公開日: 2017/12/22
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上久保原稿種別: 本文
1959 年 16 巻 p. 675-
発行日: 1959年
公開日: 2017/12/22
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上久保原稿種別: 本文
1959 年 16 巻 p. 675-
発行日: 1959年
公開日: 2017/12/22
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長沢 太郎, 葛谷 泰雄, 茂田 信子原稿種別: 本文
1959 年 16 巻 p. 676-679
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーOf the 58 quarter samples of milk analysed, 41 were definitely negative, and 17 were doubtful for latent mastitis. However, the milk from all quarters were normal in appearance. The presence or absence of infection in the individual quarter samples was determined on the basis of pH, chloride test, leucocyte count, and Hotis test of foremilk. Ascorbic acid in freshly drawn milk was determined spectrophotometrically by the 2,4-dinitrophenylhydrazine method. Difference in ascorbic acid content of individual quarter milk was not significant. The average content of ascorbic acid in milk drawn from mastitis negative quarters was 1.92 mg per 100 ml, and it was also found that in case of latent mastitis studied the ascorbic acid was reduced as much as 10 to 20 percent. About 10 percent of dehydroascorbic acid was to be found without fail in all freshly drawn milk irrespective of infection.抄録全体を表示PDF形式でダウンロード (514K) -
清水原稿種別: 本文
1959 年 16 巻 p. 679-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 679-
発行日: 1959年
公開日: 2017/12/22
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宮川 久邇子原稿種別: 本文
1959 年 16 巻 p. 680-686
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーStudies on biosynthesis of thiamine by Sacch. cerevisiae were carried out and the following results were obtained. (1) It was recognized that 2-methyl-4-amino-5-hydroxymethyl-pyrimidine or its compounds, 2-methyl-4-amino-5-aminomethyl-pyrimidine and 2-methyl-4-amino-5-formaminomethyl-pyrimidine, were good source of thiamine synthesis among various kinds of pyrimidines in existence of thiazole moiety when Sacch. cerevisiae was cultured in Reader's medium. (2) An ability of thiamine synthesis from 2-methyl-4-amino-5-hydroxymethylpyrimidine and thiazole by the washed cells of Sacch.cerevisiae which were cultured in the medium without material for synthesis of thiamine was evidently proved. The optimum pH of the synthesis was about 7.0-7.5. (3) It was also proved that the cell free extract of Sacch. cerevisiae had a complete enzyme system of the thiamine synthesis from pyrimidine and thiazole. Moreover, it was recognized that the fraction of 65% ammonium sulfate of the extract easily synthesed thiazole under the existence of ATP and Mg.抄録全体を表示PDF形式でダウンロード (713K) -
高田原稿種別: 本文
1959 年 16 巻 p. 686-
発行日: 1959年
公開日: 2017/12/22
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石井 隆一郎, 堀川 智恵子原稿種別: 本文
1959 年 16 巻 p. 687-689
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe investigation was undertaken to study the stability of carotene in the synthetic gastric juice with several amino-acids, proteins, and antioxidants. It showed that carotene was protected against oxidation by the presence of several antioxidants, i.e.butyl-hydroxyanisol, nordihydroguaiaretic acid, propyl-gallate, and ascorbic acid. Asparagine, casein, ovoalbumin, and gliadin were less effective than the antioxidants. Carotene was a little destroyed by the presence of Na-Cu-chlorophyllin, but protected by the simultaneous presence of chlorophyllin and ascorbic acid. Carotene potency does little decrease even in the strong acidity of the gastric juice when used with adequate antioxidants.抄録全体を表示PDF形式でダウンロード (299K) -
清水原稿種別: 本文
1959 年 16 巻 p. 689-
発行日: 1959年
公開日: 2017/12/22
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小野 忠義原稿種別: 本文
1959 年 16 巻 p. 690-691
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe production of thiaminase by Clostridium thiaminolyticum was tested with a cellophane tube culture method. Potent thiaminase was found to be produced in the medium.抄録全体を表示PDF形式でダウンロード (269K) -
上原原稿種別: 本文
1959 年 16 巻 p. 691-
発行日: 1959年
公開日: 2017/12/22
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堀田 一雄, 杉浦 嘉功, 小竹 淳一, 畑 晴夫, 武野 正弘, 田村 松三原稿種別: 本文
1959 年 16 巻 p. 692-693
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe riboflavin values in liver, kidney, spleen, heart and muscle of pancreatectomized dogs were found to be reduced compared with normal ones. With regard to the distribution of the 3 types of riboflavin, it was recongnized that FAD decreased a little while FMN and free riboflavin increased a little.抄録全体を表示PDF形式でダウンロード (257K) -
清水原稿種別: 本文
1959 年 16 巻 p. 693-
発行日: 1959年
公開日: 2017/12/22
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清水原稿種別: 本文
1959 年 16 巻 p. 693-
発行日: 1959年
公開日: 2017/12/22
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鈴木 友二, 田中 文彦原稿種別: 本文
1959 年 16 巻 p. 694-702
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーA microbiological assay method of vitamins in the sample containing streptomycin and dihydrostreptomycin was studied. The growth of Lactobacillus and Streptococcus used for vitamin assay, was inhibited by the existence of streptomycin and dihydrostreptomycin in relatively low concentration. Since these antibiotics are degraded with acid treatment, acid stable vitamins such as biotin and nicotinic acid are easily determined microbiologically after acid treatment. Acid stable vitamins such as pantothenic and folic acid, however, can not be assayed by this means. This paper is concerned with the microbiological assay of the latter, using antibiotic resistant Lactobacilli, which were isolated by means of successive transfer in media containing graded amounts of dihydrostreptomycin. The growth of the resistant strain was not inhibited by the presence of 1000μg/ml of streptomycin or dihydrostreptomycin, and moreover the pantothenic acid requirement of resistant strain of L. arabinosus and the folic acid requirement of resistant strain of St. faecalis were the same with that of each parent. It is preferable to use dihydrostreptomycin resistant Lactobacilli for determination of pantothenic and folic acid in the urine, excreted after the streptomycin or dihydrostreptomycin doses.抄録全体を表示PDF形式でダウンロード (807K) -
田中 文彦原稿種別: 本文
1959 年 16 巻 p. 702-706
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe growth of Lactobacilli, usually used organisms for microbioassay, was completely inhibited by erythromycin in low concentrations such as 0.1〜0.5 μg/ml, but that of erythromycin resistant strain was not inhibited even by the presence of 1000 μg/ml of erythromycin. Evidence was presented indicating that erythromycin resistant Lactobacillus arabinosus 17-5 and Streptococcus faecalis R were available organisms for determination of pantothenic and folic acid respectively in the sample containing erythromycin, for example, in the urine which excreted after erythromycin doses.抄録全体を表示PDF形式でダウンロード (523K) -
田中 文彦原稿種別: 本文
1959 年 16 巻 p. 706-710
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーA microbiological assay method of vitamins in the urine after oral dose of chloramphenicol was proposed. Chloramphenicol resistant Streptococcus faecalis R was used as test organism, because the inhibitory effect on the growth of Lactobacilli was not excluded by heat, acid or alkali treatment. The chloramphenicol resistant Stc. faecalis R was used, which was isolated by means of successive transfer in the medium containing graded amount of chloramphenicol. The maximum growth of this strain in the assay medium was one half of the parent strain, but the assay ranges of vitamins such as biotin, pantothenic, nicotinic and folic acid, were the same with the parent strain. The standard curves for these vitamins with resistant strain were not influenced even by the presence of about 200μg/ml of chloramphenicol. When vitamins in the urine after oral dose of chloramphenicol were assayed using resistant strain, good results were obtained.抄録全体を表示PDF形式でダウンロード (516K) -
清水 祥一原稿種別: 本文
1959 年 16 巻 p. 710-715
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーIt has been occasionally observed that, in some kinds of riboflavin injections, solutions became turbid during storage and considerable amounts of precipitates were formed ultimately. This research has been undertaken for the purpose of characterizing these precipitates and ascertaining the causes of the phenomenon. In most cases of preparations containing riboflavin, main constituent of the precipitates was found to be riboflavin, its reduced form or lumichrome. In some cases, the precipitates were composed of the mixture of the latter two or the decomposition product of folic acid. The finding indicates that the competent causes seem to be breakdown of supersaturated state, contamination of reducing microorganisms or action of light. In the precipitates which occurred in FMN preparations, riboflavin, FMN or lumichrome was detected, but not any reduced forms of them, except one case. The existence of riboflavin in the precipitates may be due to the use of impure FMN. The reason for that FMN itself precipitated might be adequately explained by either the limited solubility of FMN in the low pH solution as observed or formation of difficult soluble salts with heavy metal ions. The presence of riboflavin was observed as the sole component in the precipitates of almost all samples of FAD injections with the exception of one case, in which lumichrome was found.抄録全体を表示PDF形式でダウンロード (691K) -
清水 祥一, 樋口 亮一原稿種別: 本文
1959 年 16 巻 p. 716-720
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーThe incorporation of P^<32>-phosphate into FAD in the mycelium of Eremothecium ashbyii was successfully attempted. Eremothecium ashbyii was grown at 27℃ in shaking flasks, containing peptone-glucose medium. P^<32>-phosphate was added to the medium at a final concentration of 3 μc/ml. After 60 hours' cultivation, mycelium was harvested, from which FAD was extracted with a mixture of pyridine, methanol and water. The extract was purified by the following procedures : concentration in vacuo, extraction with liquid phenol, transfer to aqueous phase by adding ether, precipitation by Na_2S_2O_4 reduction, partition chromatography as well as electrophoresis on powdered cellulose column, and crystallisation by the addition of ethanol. The bright-orange powder of P^<32>-FAD thus obtained, was confirmed to be of such purity as 90% in spectrophotometric assay and entirely free from other flavins and other radioactive compounds. Its specific radioactivity, 6 μc/mg, will enable it to be useful in chemical and biochemical studies. In this method, the over-all yield of P^<32>-FAD from wet mycelium was nearly 30%.抄録全体を表示PDF形式でダウンロード (607K) -
久野 寧原稿種別: 本文
1959 年 16 巻 p. 721-722
発行日: 1959年
公開日: 2017/12/22
ジャーナル フリーPDF形式でダウンロード (343K)