Immune sera were prepared employing standard Candida strains, which are at present adopted by Research Council for Fungus Diseases and consist of 7 Candida strains including Candida albicans. With these sera the cross agglutination test and the cross absorption test of agglutinin were carried out for the purpose of antigenic analysis. According to TSUCHIYA et al. (1954), the antigenic factors were given in Roman numerals in this report. The antigenic structure of each strain was represented as follows:
C. albicans I II III IV V VII VIII C. krusei I II V VI VII C. parakrusei I II III VII VIII XI C. tropicalis I II III IV VII VIII C. pseudotropicalis I V IX C. stellatoidea I II III VI V VII C. guilliermondi I II III VT X
These results were in agreement with those by TSUCHIYA et al., except for the demonstration of V. which had been already known with C. krusei, likewise in C. albicans.

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The origin of intoxication in ekiri was sought by the author not only in dysentery infection itself, but also in the intestinal flora of morbid children. Tyramine production by the intestinal flora was investigated, because the severe intoxication phenomena of this disease were assumed to be incited by tyramine liberated by intestinal flora and put into the circulation undetoxified, as a result of hepatic injury caused by dysenteric infection.
1. The stool of 25 ekiri and 20 dysentery patients was examined for the presence of tyramine producing strains by means of paper chromatography. Tyramine producing strains were demonstrated in all ekiri cases examined and occupied on an average 34.4% of total isolated strains of intestinal flora. On the contrary, only a small number of dysentery patients excreted tyramine positive strains, and the proportion of the latter to the total isolated strains gave a low average value of 14%. Dysentery bacilli, isolated both from ekiri and dysentery cases had no tyramine producing activity.
2. The amine liberating activity of 8 tyramine positive coli strains was compared to that of 5 tyramine positive standard coli strains. The former generally exhibited quantitatively higher amine producing activity than the latter, with which only a minimum amount of tyramine was demonstrable. In this experiment tyramine was separated and extracted with ion exchanging resin IRC50 and determined electrophotometrically after Maziac-Schoental's method.
3. The distribution of tyramine positve strains in the intestinal canal was examined at the autopsy of 2 ekiri cases with typical intoxication symptoms and autopsy findings. A large number of tyramine positive coli strains was demonstrated from the colon up to the lower and middle part of the ileum, whereas, in an autopsy case of dysentery, such strains were neither isolated from the stool, nor from any part of the intestinal canal. In another dysentery case with clinically positive bacillus discharge the distribution of the bacilli was restricted to the colon, as revealed by bacteriological examination at autopsy.

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Electroshock treatment was introduced in the therapy of 14 encephalitis patients with mental disorders, after the lapse of the acute stage. The results were as follows.
1. The most satisfactory results were obtained in 4 cases, somewhat less remarkable effects were confirmed in other 3, recognizable but still less effects in other 6 and no therapeutic influence in the remaining one.
2. Neither aggravation of symptoms nor side effect was induced by the treatment.

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The mutation among 4 species of Enterococci, St. fecalis, St. liquefaciens, St. zymogenes and St. durans has not hitherto been experimentally demonstrated, although they are closely related to one another.
The author could endow the capacity of β-hemolysis to St. fecalis, by means of successive transfer of the cocci together with a minimum amount of aseptic staphylotoxin in the testis of mice belonging to the dd-strain. This mutation was confirmed with 5 strains of St. fecalis, and the hemolysis thus induced was characteristic of that of the II-type of Kobayashi's classification (positive β-hemolysis both on horse and sheep blood). These 5 strains underwent mutation to the III-type of Kobayashi (β-hemolysis on horse blood, and α-hemolysis on sheep blood), when they were seeded on the “total heat sterilized fluid culture of original strain (St. fecalis) ”. The same mutation could also be induced in 4 of the 5 above strains within about a year, when the cocci belonging to the II-type were successively preserved on blood agar slants.
These strains of II-and III-type showed resistence against antibiotics and following typical biological properties of enterococci. They grew in 0.1% methylen blue milk, 6.5% NaCl broth and in the broth at pH 9.6. They were furthermore relatively thermostable, resistant to potassium tellulite, could ferment Mannitol and Esculin and did not produce “soluble hemolysin”.
The mutational strains were indistinguishable from the original strains by precipitation reaction and belonged likewise to the group-D of Lancefield.
All the mutational II and III strains were therefore identified as St. zomogenes and the mutation of St. fecalis to St. zymogenes was thus demonstrated.
According to Kobayashi, hemolytic enterococci should always belong to the III-type, but the author's experiment revealed their possible classification into the II-type. This must be taken into consideration in the application of the classification of Kobayashi.

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