Few reports are available at present, to the extent of the Author's knowledge, concerning the fluctuation of serum protein pattern in the course of Japanese B Encephalitis (JBE) in the correlation with HI antibody level. In this respects, this experiment was undertaken.
Having adopted as a serological standard the criterion set up by the Study Team for JBE (1963, 1964) with some modification of the Author's own, the Author tried, in this paper, the comparison between electrophoretical findings on cellulose acetate membrane and serological findings in the sera of 83 patients admitted to Higashi Shimin Byoin (City Hospital), Nagoya City, during a 2-year period (1964-1965) under the diagnosis of JBE or its suspect.
Thus the patients were divided into following 5 groups:
Group I (22 cases): died in acute stadium, with apparent clinical manifestations of JBE
Group II (25): showing (+ +), (+) in the criterion of the Study Team (4-fold or higher rise of HI, with the highest-≥ 1: 160, in paired sera, or if not paired, the highest-≥1: 320)
Group III (5):(±) in the criterion (4-fold or higher rise of HI, with the highest-1: 80 in paired sera, or if not paired, the highest-1: 160)
Group IV (9)(-) in the criterion (2-fold rise of HI, with the highest-1: 80 in paired sera)
Group V (22):(-) in the criterion (2-fold rise of HI, with the highest-≤1: 40 in paired sera)
(Group IV and Group V were separated in the Author's consideration.)
Variations of cellulose acetate electrophoretic protein patterns in each group were scrutinized and compared with each other.
Conclusions obtained were as follows:
1) There is a common tendency in the fluctuation of serum protein pattern in Group I, II, III, and 1 within 3 weeks after the onset of illness. That is, statistically significant decrease of albumin, increase of α₁-globulin, noticeable increase of α₂-globulin, staying in normal limits of β-globulin and γ-globulin.
2) Group II, III and IV were very similar in clinical findings and serum protein pattern.
3) Group V remained electrophoretically within normal ljmits.
4) The fluctuation of serum protein pattern mentioned in 1) can be considered as characteristic to JBE in statistical viewpoint. Therefore, this is believed to be herfulp for diagnosis of early death cases, in which serological confirmation is not yet possible.
5) Group IV was separated as the highly suspectable of JBE on account of its manner in electropho-resis. Reconsideration should be given to this group.

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A 26-year-old house wife developed high fever, ranging 39°C-40°C two days after spontaneous abortion of unknown etiology occurring in 3rd month of her pregnancy. On clinical examination, diplopia, stiffneck, Osler's Spot in the tips of fingers, and systolic murmur over the mitral valve were noted.
Blood culture, performed on admission, 10th day of illness, using thioglycolate broth media gave positive result for Staphylococcus aureus (ST. aur.) of coagulase-positive.
Sensitivity test (disc method) of this strain to antibiotics revealed its high sensitivity (+) to such drugs as tetracycline (TC), erythromycin (EM), oleandmycin (OM), and Kanamycin (KM).
High fever, having durated for about three weeks, began subsiding after the administration of these drugs. But, shortly after the discontinuation of TC administration, 40th day of illness, the patient again developed high fever above 40 C.
St. aur. was again recovered from the blood on 45th day of illness, and the drug-sensitivity of it was found this time much different from the previous one. Only to TC it remained stillhighly sensitive (+), but dropped off the sensitivity to EM, CM, and KM.
During her hospitalization, she was given 46.25 g of oxytetracycline orally, and 21.5 g of pyrolidinomethyl tetracycline intravenously in total. She became free of fever since 55th day of illness and was discharged on 120th day of illness, having slight mitral valve damage as a sequela. She has been healthy for four years since then.

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Amidst the prevalence of bacillary dysentery, it bears the primary importance to enhance an isolation rate of causative agents from individual patients. For this, the contrivance of better culture media should be indispensable.
On the other hand, there has been an ever increasing number of antibiotic-resistant strains of Shigella sonnei, which have been most frequently the causative agents of recent outbreaks.
Considering such circumstances, the Author contrived a new medum, a new type of enrichment medium for Shigella, particularly Shigella sonnei, containing selenite and 50 γ/ml chloramphenicol.
The clinical application yeilded the excellent result that any other medium has hitherto never provided.
Its further clinical trials by others are hoped to be done.

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