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Volume 31, Issue 12
Displaying 1-3 of 3 articles from this issue
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Hitoshi SUCHI1958 Volume 31 Issue 12 Pages 629-633
Published: March 20, 1958
Released on J-STAGE: November 25, 2011
JOURNAL FREE ACCESSThe antibacterial effect of novobiocin, a new antibiotic was investigated in comparison with other antibiotics. In general, it was effective against gram-positive bacteriae. The results were summarised asfollows:
1) Novobiocin was most effective against staphylococci among the antibiotics in common use and ranked next to penicillin and erythromycin in the activity against Streptococcus haemolyticus.
2) It was situated between penicillin and erythromycin in the antibacterial effect against Corynebacterium diphtheriae.
3) The above mentioned three microorganisms showed neither novobiocin-resistance, nor cross-resistance with other antibiotics.
4) All gram-negative intestinalflora displayed over50γ/cc resistance, the majority being over 100γ/cc.
It was thus concluded that novobiocin possessed the highest order of activity against staphylococci among the commonly used antibiotics. It could be recommended as a drug of choice in the treatment against staphylococci.
Further investigations are required for the problem concerning the acquirement of resistance of various organisms, notably staphylococci against novobiocin.View full abstractDownload PDF (757K) -
Significance of bacterial enzymes in ekiri. ISeizo KOZAKI1958 Volume 31 Issue 12 Pages 634-636
Published: March 20, 1958
Released on J-STAGE: November 25, 2011
JOURNAL FREE ACCESSHistamine, agmatine, cadaverine, putrescine andry-aminobutyric acid were detected from the stools of 6 ekiri patients by the ion exchange resin method andpaperchromatography.
The stool was poured into the column of ion exchange resin “amberlite IRC 50 and” the absorbed substances were eluted with 2NHCl.
The eluted substances were again poured into the column of ion exchangeresin. “amberlite XE 64”, and the absorbed substances were eluted fractionally with aceticacidacetate buffer solution at pH 5.2. The final substances were condensed anddeveloped with butanol acetate on a filter paper and sprayed with ninhydrin.
The Rf values and colour of the spots indicated the existence of the abovementioned amines.
It was assumed that the activities of the amino acid decarboxylases of thepathogenic microorganism in the intestinal canal of ekiri patients might have produced the amines.
The significance of the amines as an exogenic factor in ekiri was discussed.View full abstractDownload PDF (391K) -
The Significance of Bacterial Enzymes in Ekiri. IISeizo KOZAKI1958 Volume 31 Issue 12 Pages 637-639
Published: March 20, 1958
Released on J-STAGE: November 25, 2011
JOURNAL FREE ACCESSThe distribution of amino acid decarboxylases was investigated with 10 strains of Sh. dysenteriae. Each strain was inoculated into the meat extract broth (pH. 7. 2) containing 2 per cent glucose, 100r/ml pyridoxine, and 0.2 per cent amino acid (substrate). After 18-24 hours incubation at37°C the cells were harvested bycentrifugation, washed, resuspended in. the acetic acid buffer solution containing amino acid (substrate) in M/20 concentration, and incubated for 24 hours at 37°C. Thereafter the mixture was treated with alcohol, centrifuged, and the precipitated protein was discarded. One drop of the supernatant was developed on the paperchromatogram and the ninhydrin spots of the amines were examined.
Histidine decarboxylase was demonstrated only in Hanabusa-and Yamadastrains of Sh. dysenteriae I, ornithine only in 1199 strain of Sh. dysenterias I, arginine, lysine and glutamic acid decarbosylases in all strains, whereas tyrosine decarboxylase was found in no strain.
Accordingly, there were only afew among the 10 strains of Sh. dysenteriae, which could decarboxilize histidine and ornithine. None of the 7 strains sent from U. S. A. exhibited the ability of decarboxylizing histidine. This ability was-observed only in 2 strains isolated in Japan.View full abstractDownload PDF (406K)
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