Studies on clinical pictures, radiographic findings, or antibody measurements of Mycoplasma pneumoniae (Mp) infection have been accumulated and outline of this disease is becoming clearer.
However, there still remained a lot of unsolved problems about it, particularly in the details of its, infectivity, manifestivity, pathogenesity to the lung, and histopathological findings of the lung.
As of the present time, because of mildness, chronicity, and extremely low mortality of this disease in man, much cannot be expected beyond experimental studies to approach these problems.
In this regard, an experiment elaborated by the author's own methodology was carried out and evaluation of the results was attempted in this paper.
Animals chosen after preliminary experiments as the most favorable are young hamsters of 3 weeks: old, and an infection method chosen as the most reliable is spray-inhalation method using 30 ml FH-strainsuspension-broth of 5.0×10⁶ colony-forming-unit/nal for 30 minutes. The number of animals used is. 60 and counting of intrapulmonary Mp was done at 1, 3, 5, 7, 10, 14, 21, 28, 35, and 42th day after the, infection.
Further, the isolations of the organisms from the pharynx, measurement of antibody (CF and GI antibody), histo-pathological and fluoro-immuno-pathological studies of the lung, chest X-ray examinations, and hematological studies were performed on the animals.
The results were summed up as follows:
1) Isolation rate from the pharynx: first day; 63%, and 2nd day; 29%, then on upward trend to 100=% on 10th day. Decrease on 2nd day is not coincided with quantitative measurements of intratracheal. or intra-pulmonary Mp. The meaning of it is unclear.
2) Counting of intra-pulmonary Mp: 1st day; 10⁴ strong CFU/ml, 5th day; 10⁵ strong, 7th day 10⁶ strong, after 10th day till 6th week; around 10⁵. Thus, long term residing of Mp in the lung was confirmed.
3) Histo-pathological findings: tracheitis, peritracheitis and interstitial pulmonitis were observed. and its intensity was almost pallarel to the value of intra-pulmonary Mp counting. Specific immuno-fluorescene of Mp was observed only in 2nd week along with the tracheal epithel layer.
4) Only a few cases showed I: 8 or 1: 16 CF value between 3rd and 6th week. This is not significant in contrast with the level of intra-pulmonary colony counting. No appreciable rise of GI value at all. This discrepancy has to be studied further.
5) In the light of somewhat unexpected results of immunological experiments, the author further investigated chest X-rays, CRP value, protein fractions, and hematological findings. No significant differences were observed in chest X-ray, CRP, and protein fraction between the infection animals and the contrast group. In hematological findings, noticeable neutrophilia, particularly an increase of segmented neutrophils, was recognized. These results apparently tell complicity of living reactions in such an infection experiment, particularly of such mild organisms.

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An epidemic of rubella occurring among recruits of the Japanese Ground Self-Defence Forces (JGSDF) at Ohtsu Base Camp in Ohtsu city in a period from the middle of May to the end of July 1967 was outlined in the previous report, Report 1., in this magazine.
In this paper, the results of sero-epidemiological observations were presented.
1) Hemagglutination-inhibition test of rubella viruses was carried out on 12 cases who initialed this epidemic. In them, significant rise of the antibody was shown between acute and convalescent stage. In spite of the negative results of virus isolations performed on these patients in the acute stage, the seroimmunological evidence in them is strong enough to support the confirmation of their rebella infection.
2) At the very beginning and the end of the epidemic, a half number of all recruits belonging to two companies, in which the disease was prevalent, was extracted by random sampling and tested for HI level.
Antibody possessing rate was 61/121 at the beginning of the epidemic and turned to 116/121 at the end in one company, and 66/145 turned to 145/145 in the other. According to this result, the authors estimated that almost 50% of recruits at Ohtsu Base Camp had already possessed the antibody before the epidemic, and almost all the members were infected with the viruses by the end of the epidemic.
3) In this epidemic, the ratio between the latent and the manifested among supposedly newly infected cases was estimated about 1: 1.

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Jasomycin, one of macroride group antibiotics, was used orally in the treatment of iatent syphilis in an expectation that it would have antisyphilitic effect already shown by other macrorides like erythromy-cin. The number of patients was 13 in all, and daily dose of 1, 200 mg Jasomycin was administrated 3 times a day for 4 weeks in almost all cases. Serological tests for syphilis (STS) were performed before, during and after the treatment. The methods of STS used were Wassermann Reaction by Ogata's modification, VDRL and TPHA.
The results were as follows:
In all cases save three, the antibody titers were fluctuated, but, as general trend, decreasing in the titer after the treatment prevailed.
In one case whose cerebral conditions necessitated additional use of steroid, STS turned to negative during the course of the treatment. In that case, the influence of steroid to the im.muno-reactions should be taken into consideration.
Three out of four cases with positive STS in the spinal fluid taken before the treatment showed its turning to negative after treatment

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