The pathogenicity of virulent strain B. of rinderpest virus which has been maintained by passages in cattle for these forty years, and 3 attenuated viruses obtained from the Bstrain, lapinized strain L, avianized strain BA and lapinized-avianized strain LA were compared in respect to cytopathogenic effect and production of complement fixing antigen in variou cell cultnres originated from cattle, rabbits and chick embryos.
Results are summarized as follows:
1. By the inoculation of the B, LA and BA viruses were obserued degeneration of cellsfrom cattle and chick embryos (bovine embryos kidney, bovine testis, bovine kidney, chick embryo kidney and chick embryo skin-muskel mixture) and production of complement fixing antigen inthem. The pathogenicity of strain B seemed to be lower in the chick embryo cells than that of avianized strains. On the other hand, the inoculation of the L strain flailed not only to show degeneration of cells detectable by the examination. of unst ained cultures but to produce complement fixing antigen in these cells. Never theless, the L virus readily acquired the ability of multiplication accompanying the characteristic degeneration of cells and production of complement fixing antigen by passages in bovine embryo kidney cells.
2. No cytopathogenic changes and production of complement fixing antigen were observed in rabbit kidney and rabbit embryo kidney cells by the inoculation of any strains, except slight degenerative changes detectable only in a few of stained cultures inoculated with the B and LA strain. These cells seem to be lowor unsusceptible to the viruses.
3. Cytopathogenic changes _ consisted of necrosis and syncytium, following the formation fo multinuclear giant cells and eosinophilic cytoplasmic and intranuclear inclusion.
The proportion of necrosis and syncytium in a culture seemed to be dependent upon the kind of cells ; e. g. necrosis was prominent in primary cultures from animal tissues and syncytium was prominent in continuous cell cultures.

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The form variation of Salmonella, especially in S. pullorum, exhibit rather complexones, and it has been recognized that the factor 12₂ would play its main role.The author attempt to establish the diagnostic standpoint of pullorum disease byascertaining those response behavior of blood antibodies in fowls infected with “Variant” as well as “Standard” strain of S. pullorum against the respective factors.
As the results obtained, the majority of adult ones in infected bulk with variant and standard strains indicated the same reactive forms against and variant standard antigens.
Chickens manifested, however, notable tendencies of special response form against these two different antigenic formula respectively. Especially, in those infected with the variant strain., these were markedly distinct.
In chickens, such being the case discribed above, unlike the adult ones, it was pointed. out that antibodies produced by result of the infection of variant strain were occupied by those factors corresponding 12₂, and those of standard strain, by the fators corresponding 12₃.
It was ascertained, that there were few antibodies corresponding 9 and 12, ₁ factors which are common for every strains of S. pullorum.

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Using the anthrax serum highly immunized with living bacilli, the author made the study estimating passive immunity against anthrax by means of animal test and electorophoratic analysis of immunserum.
The results obtained are described as follows. Using mice and hamsters as the test animal, the protective ability of immun-sera in those animals were not confirmed, while in guinea pigs it was observed in low degrees. The higher protection rate of passive immunity was, however, recognized in rabbits and some of them survived against the challenge with high-virulent anthrax bacilli.
In the electrophoratic analysis of irnmun-serum β2-globulin appeared in serum at the stage of establishment of higher immunity, and total protein of immun-serum increased gradually in consequence of progress of immun procedure.
The serum in which appeured β2-globlin exhibited the establishment of passive immunity in rabbits.
In views of these facts, it may be suggested that the protective antigenicity of anthrax immun-serum should be related closely to the presence of β2-globulin in serum.

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