A 20-40% emulsion of chorio-allantoic membrane and fetus of hen egg infected with mouse-adapted measles virus (Ohki-strain serially passed through mouse brains 22-29 generations) was ultracentrifuged ca 100, 000G and concentrated to 100-200x (LD50: 10-6.5-10-7/0.02 ml against mouse). The signs and symptoms similar to the human measles were caused in the monkeys (M. irus 5, M. fuscatus 1, M. mulattus 1) without any experience of measles infection, when injected with 0.6-4 cc/kg of the above mentioned emulsion.
The erruption comparable with the human one was recognized in all the monkeys. except one, killed at the time where enanthem appeared on the mucous membrane. All of the monkeys showed fever and enanthem on the mucous membrane which was, entirely identical with the Koplik's spot in 3 of them. The incubation, the time period. from the injection to the appearance of Koplik's or other spots on the mucius membrane was 6-10 days (average 8 days), and the time period from the injection to the appearane of rash was 12-14 days (average 12 days). The leucocyte count increased in the early incubation period and decreased before or at the height of the disease. The majority of the monkeys demonstrated conjunctivitis, rhinitis and anorexi4, and some of them suffered also from diarrhoea, bronchitis, etc.

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Studies on the nutrition of dysentery bacilli led the authors to the following interesting results:
1. A watery extract of pancreas autolysate contains a strong growth-promoting substance for dysentery bacilli.
2. A watery extract of pancreas autolysate was effective for the multiplication of type I of Sh. boydii even after the dilution at a rate of 5120x the weight of the pancreas.
3. Extracts of other fresh or autolytic organs were not effective as that of pancreas autolysat.
4. According to the analysis performed, the major effective ingredients of the latter proved to be biotin and uracil.
5. Type I of Sh. boydii was found to be a strain which needs biotin for multiplication.

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N/25 ammonia water is used for elution of virus absorbed by kaolin in the purified vaccine lymph after Yaoi's method. For the neutralization of lymph, acetic acid is added in the original method. Anhydric carbonic acid, however, was used in our method. The titer was examined with each of the lymphs which were preserved immediately after the lyophilization for 50 days, 100 days, 0.5 year, 1 year, 1.5 years and 2 years in 35°C and the ice room. All the lymphs neutralized with anhydric carbonic acid were preserved in a good state.
Electric discharge as a test method for a perfection of welding did not exert any unfavorable influence on the titer of vaccine lymph in the vacuous ampule.
Substitute plasma, polyvinylpyrrolidon and sodium glucuronic acid were not good protective substances. Lyophilized purified vaccine lymph added with 1% lactalbumin, when preserved in a vacuous ampule was not influenced by a heating at 100°C for an hour.

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Intravenous injection of typhoid vaccine immediately induces, as is known, leukopenia in the peripheral blood of rabbits, followed by leukocytosis in 6-10 hours. In these days however, no leukocytosis has been observed by the author following the injection of typhoid vaccine. It became known that this variation was caused by the use of typhoid chrome vaccine instead of formalin vaccine. Stimulated by this fact, the following experiments were carried out to study the cause of leukocytosis.
By the intravenous injection of 0.5-1.5 ml formalin vaccine pro kg of body weight to rabbits, the number of leukocytes decreased immediately in the peripheral blood, increased then gradually in 1-3 hours and markedly in 6-10 hours, to reach the count 3 times as great as that before the injection. However, the chrome vaccine i. e. formalin vaccine treated with 0.02% alum chrom. for 72 hours induced leukopenia, but no clear leukocytosis. Nevertheless, the chrome vaccine, when' heated 100°C for one hour brought about immediately leukopenia and in 9-12 hours a marked leukocytosis as did the contrast formalin vaccine.
Regarding the toxicity, the chrome vaccine proved much less toxic against the mice than the formalin vaccine. However, the chrome vaccine restored its toxicity by the heating at 100°C for one hour, showing almost the same mortality rate of rabbits as the formalin vaccine.
From these results it was concluded that the toxicity of typhoid vaccine was somehow related to the leukocytosis but not to the leukopenia.

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