CF test for influenza A and HI test for A/Asia/57 and A/Kojiya/52 were carried out with 442 paired samples of sera obtained from infants suffering from grip from April. 1957 to May 1958;
The results were as follows:
1) A/Asia/57 was isolated in 106 A/Kojiya/52 belonging to the strains hitherto known in two cases.
2) A/Asia/57 occurring since May 1957, demonstrated two peaks in June and Nevember in this year and then gradually decreased till next April.
Two cases were caused by Kojiya strain, one in January and the other in February-1958.
All age groups including one case of newborn were involved, demonstrating a tendency of predominance in school boys.
4) Compared with HI, a significant increase of CF antibody titer was in many cases recognized till 2 weeks of illness. No significant rise of CF antibody titer was noticed in sucklings and when noticed, fell in 6 out of 7 cases to the value of acute stage by 120 days of illness.
5) HI antibody titer rose more slowly than that of CF and 7 out of 10 cases; demonstrated the fall to 32 x by 150 days of illness.
6) A significant rise of cold hemagglutination value was observed only in 2.8% of A/Asia/57 cases.

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Hundred and six cases of influenza A/Asia/57 diagnosed by the serological tests reported in the previous paper were divided into A group (78 cases in the hight of epidemic) and B group (28 sporadic cases before and after the epidemic).
The clinical aspect was as follows:
1) Statistically significant differences between the two groups consisted in that a sudden onset with initial fever was more frequently encountered in the A group and more cases free from fever in the B group.
2) Diagnoses suspected were in the following order ; pharyngitis, tonsillitis, bronchitis, rhinitis, laryngitis and penumonia. Bronchitis was observed in about 20% of A as well as B group. Slight cases such as rhinitis was observed in 2 cases of A and in 3 cases of B group.
3) The majority of both groups had a fever period within 4 days, demonstrating a fever curve with 2 peaks in 12 out of 73 cases (16.4%) and a curve with 3 peaks in one case.
4) The majority of cases recovered within 2 weeks, no difference being noticed between the two groups.
5) Twenty two cases were complicated by nephritis (10 cases), periodic vomiting (3 cases), otitis media, exacerbation of pulmonary tuberculosis (2 cases), dyspepsy (2 cases), serous meningitis (1 case), cervical lymphadenitis (1 case) and pyothorax (1 case).
6) Abnormalities of leukocyte-count and figure and blood sedimentation rate were seen more frequently in the cases with complication or in the cases with pulmonary changes discovered roentgenologically.
7) Four cases of primary atypical pneumonia and one case of pleuritis were observed roentgenologically (5 cases in total, 4.7%) and, 2 cases of bronchitis were also seen.
8) Bacterial flora was investigated for the study of secondary infection with the following results; staphylococci with positive coagulase (34.5%), streptococci (10.3%), pneumonial diplococci (12.4%) and influenza bacilli (6.3%).
9) Antibiotica proved to be effective as compared with the contrast group.

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Endocrine organs of 16 cases of “ekiri” were histologically investigated. The principal findings were found in the adrenals. Thymus and hypophysis were also damaged.
The adrenal findings consisted in hyperemia, hemorrhage, necrosis, atrophy and dissociation of cortex cells, decrease or disappearance of lipid, or Keto-steroid.
Ganglion solare was histologically investigated in 6 cases of “ekiri” and one case of septic dysentery. Degenerative atrophy was found in ganglion cells, which led us to the conclusion that the peripheral sympathic nervous system was involved in “ekiri”.
Some cases demonstrated changes in adrenal as well as in ganglion solare, showing almost the same severity, so that the influence of vegetative nervous system on the adrenal might be assumed.
Adrenal insufficiency was regarded as a factor in the developmental mechanism of “ekiri”.

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Landis method was adopted by the author in order to investigate spasm and rise of permeability of the peripheral blood vessels, said to be present at the height of Ekiri, for the study of developmental mechanism of this condition.
First the validity of Landis nethod was assessed, employing infants completely recovered from dysentery as hea thy contrasts.
Primarily the related distribution of the amount of fluid filtrated (X (cc)) and the amount of albumin filtrated [ΔPr (g)] on the same table and that of the fluctuation rate of Ht [Htn-Htu/Htu (%)] and of albumin concentration [Δcpn (%)] were investigated. In certain areas of both tables, a physico-chemically quite unexplainable conclusion, i. e. an invasion of concentrated albumin fluid into the blood stream in result of pressure on vein, would have to be drawn, if one adopted the idea of Landis uncritically.
Secondarily the postulation of Landis that the total volume of erythrocytes remains before and after the pressure had to be given up according to our results of investigations, the wide range of variation of Ht/R and Hb/R values and the increase of Hb/Ht, though quite little.
From these data it may be pointed out that the idea and calculation formulae of Landis, standing on a theoretically weak ground, do not conform with the biological phenomena themselves. In the analysis of Landis phenomenon more consideration for the biological phenomena themselves on a better established ground must be required taking into consideration the variation of plasma and the shift of total volume of erythrocytes.

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Serial investigations have been made to find out suitable protective substances, by the addition of which lyophilized purified vaccine lymph prepared by Yaoi's method would become more stable and enable us to distribute the purified vaccine virus in a more practicable state to practitioners.
Among various chemicals subjected to the present investigation, lactalbumin showed the most preserving effect for vaccine virus followsd by lactose, saccharose and gelatine. Sodium glutamic acid has been estimated sometimes to be by far inferior to the substances mentioned above. Furthermore, germs increased very much by the addition of sodium glutamic acid in the lyophilized pulp. In contrast, when the pulp was added with gelatine and lyophilized, the number of germs did not increase.

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Adenovirus was isolated from the infants treated in our hospital as grip in period from March 1957 to October 1958 using Hela cell.
The results were as follows:
1) The adenovirus was isolated from 16 out of 244 cases investigated, i, e, in 6.5 %.
2) These 16 strains were identified as 1 strain of Type 1 (6.3 %), 10 strains of Type II (62.5), and 5 strains of Type III (31.2 %). In 1957 Tpye III was more isolated, while Type II alone was isolated in 1958
3) Regarding the clinical diagnosis, acute pharyngitis was most frequently encountered (7 cases), and acute tonsillitis next frequently (4 cases). Each one case of acute tracheitis. acute rhinitis., suspected scarlet fever and dyspepsy with transient nuchal paralysis.
The clinical course of the last case was described.
4) The isolation rate was highest in the beginning of the disease. Within 1-6 day of the illness, the virus was isolated in 14 out of 16 case (87.5 070). The rate was higher in rinse water than in rubbed material from the throat.

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Neutralisation test was carried out against each type of adenoviruses with 13 cases which demonstrated a significant rise of complement fixation test in the convalescence. The distribution of serum anbibody against I-VII types according to monthly age was investigated with 50 cases of sucklings under 2 years of age observed from March to June 1957. The results were as follows
1) The type could be decided in 8 out of 13 cases of adenovirus infection. Three cases were Type II and 5 wre Type III.
2) Type II was found always in acute pharyngitis.
Type III was identified in 4 cases of acute pharyngitis and in one case of acute bronchitis.
3) The antibody titer demonstrated the same fluctuation in all types except IV and VII, i. e. indicating the highest value till 3 months after birth, declining between 6-12 months and increasing again in 13-24 months. Type IV showed no tendency of re-increase even in 13-24 months, though in 7-12 months the same pattern of fluctuation was recognized.
4) As for the number of antibody types, infants under 6 months demonstrated 1 to 7 types, respectively, almost a half possessing more than 3. The majority of infants of 7-12 months possessed one or two types, one case out of them demonstrating no antibody. However, 113 of infants of 13-24 months pessessed more than 3 types of antibody.

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