Thirteen strains of red type salmonella cultivated on normal agar media were studied in contrast with white type strains on their immunological properties and on a relationship between these properties and the growth-impeding activity of fuchsin. Furthermore, immunological properties of red type, when cultured on the Endo-medium were examined.
Cultivated on normal agar media the red type demonstrated no definite difference in these properties as compared with the white. On the Endo-medium the red type showed increase of its natural agglutinOility, augmentation of O agglutination titer, decrease of H agglutination titer and fall of precipitation. The white type remained uninfluenced.
In the white as well as in the red type, when their growth was inhibited by fuchsin, the natural agglutinability augmented in compliance with the increase of O agglutination titer and the decrease of H agglutination titer, and the precipitation test converted to negative.
These data suggest that the variation of red type on the Endo-medium was brought about by the growth-impeding activity of fuchsin and the specific appearance of these variation in red type was referable to the differential resistance of both types to fuchsin.
Agglutinin in the 0-and H-serum was well absorbed by the bacilli of both types cultured on the normal and Endo-medium. And between both types on these media no difference of antigenicity (immunizing power) was observed against the rabbit.
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