One thousand three hundred and fifty one strains of Shigella sonnei isolated mainly in Nagasaki and Saga prefectures within a year from May 1965, were subjected to the colicine typing. All strains were tested by Naito's method, and 109 of them were tested by Abott and Shannon's method in parallel.
The test by Naito's method revealed that B₂ was an absolutely predominant type over all occasions except one caused by subtype C1. On the other hand, by Abbott and Shannon's method it became obvious that some outbreaks and some districts were predominated by Type 6, 6/11, and 11, though type 4, 4/14, and 14 were prevailing over vast majority of the occasions.
So, from epidemiological viewpoint, it should be admitted at this stage that Abbott and Shannon's method is more valuable than Naito's method.
Besides these experiments, typing of 30 strains originated from Taiwan, Hongkong, Korea, and the Philippines, of 74 stock cultures isolated in Nagasaki prefecture in 1959, and of 2 standard strains of Shigella sonnei was performed by the both methods. It is noteworthy that Abbott and Shannon's Type 9, which can not be enrolled in Naito's classification prevails in Hongkong
The relation between each type classifiable by the both methods was also discussed in this paper.

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The serum specimens collected, during a period of 1964-1965, from 360 persons living in three different districts in Fukushima prefecture were examined quantitatively for the neutralyzing antibody against adenovirus Type 3. The antibody titers obtained were analyzed concerning age distribution, area, and year.
The following results were obtained:
1) Increase of antibody possessing rate was seen in the course of an age span from 3 to 15. Particularly, the increase was remarkable between 6 and 12, so-called at school-age. About 80% of persons examined were proved to have acquired the antibody within less than 15 years of age.
2) The antibody possessing rate seen in children of pre-school age in this prefecture was considerably lower than that in Okayama or Tokyo district appearing in published papers.
3) The antibody possessing rate was proved to differ according to area. In children under 10, it differed appreciably according to the year of experiment.
4) Out of persons possessing the antibody of over 1: 4 dilution level, 35% were positive at 1: 16, and 15%, at 1: 64. In case of school children, 30% of them were positive at 1: 16, and 27%, at 1: 64. As a whole, the antibody titers of school children were higher than those of adults.

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The status of fish-borne Vibrio parahaemolyticus (V. P.) in the course of fish marketing in Miyazakicity and Nobeoka city, Miyazaki prefecture, from its landing through wholesale fishmarket to retailer shop, and the possibility of its secondary contaminations to other foodstuffs were investigated at the following four periods: two in hot season July and Sept., 1965, two in cold season-Nov., 1965 and Feb., 1966.
The items subjected to V. P. culture were as follows:
1) At the time of landing-the surface of fish of some given lots, water from fish-storage tanks of the fishing boats, some amount of sea-water and the mud of sea-bed at the pier.
2) At the wholesale fish-market-the surface of the fish, waste water, and the case-boxes belonging to the same lots as mentioned in 1).
3) At the retailer's-the surface of the fish of the same lots as above, knives, chopping boards, fishcontainers, refrigerators, and dish towels.
The results obtained were as follows:
1) No V. P. could be detected from any materials in Nov. and Feb. This would be compartible with the facts that no food poisoning due to V. P. ever took place in winter time, and no V.P. were found from the stool of diarrheal patients in that season.
2) To the contrary, 3 strains, 1 from fish and 2 from its surroundings were isolated in June. Thirty eight strains, 35 from fish and 3 from surroundings, in Sept.; A sharp increase of the incidence in late summer was impressive
3) Contaminations of V P. to the surroundings were observed in a few incidence in September. However, observations due to V. P. colony counting showed that the multiplication of V. P. of serologically identical type never took place at any stage of fish circulation.4) Only one strain among those isolated in this experiment was positive for the hemolysis test. And none of them coincided serologically with the strains isolated from the patients of food poisoning in 1965. About 80% of them were untypable unlike that almost all strains derived from patients were proved to be typable. These facts are suggestive of rare incidence of pathogenic V. P. in the natural world.
5) It is inconceivable that under the usual cold-chain system in this prefecture, the cause of food poisoning due to V. P. shculd occur in the course of fish circulaton from its landing to fish retailer's

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