A 42 years old man was suspected of suffening from psittacosis. The disease began with a continuing high fever and diarrhea. The fever subsided by chloromycetin in 4 days. Recurred signs and symptoms such as coughing, sputum, ronchus at the right lower back and fever were benefitially combatted by another application of 8 grams of chioromycetin.
All the clinical investigations aiming at typhoid fever failed. Atypical pneumonia was next suspected from a series of X ray examinations showing a marked shadow on the right hilum. Cold hemagglutination test by O-type human erythrocytes, however, was negative.
On the other hand, the complement fixation test for psittacosis turned positive in 32 times dilution on the 30th day and the 68th day, the antibody titer coming down to 8 times on the 166th day.
It was learned in addition that the patient had kept many birds including a parrot before falling ill. He had not only looked after them, but also kissed them directly. Birds died successively after the breakout of the disease. These facts together with the above montioned clinical observations would be enough to diagnose psittacosis.

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Sero-epidemiological studies on the latent infection of japanese encephalitis were performed on the population in a certain area (Tsukurimichi, Toyama prefecture) by means of complement fixation and neutralisation test. The results of theses tests were analysed from a clinical, sero-epidemiological and statistical standpoint and their positive boundaries were fixed.
1) As for the specifity of complement fixation test, no relationship was recognized between japanese encephalitis and viral diseases such as polio, parotitis and syphilis or other diseases.
2) Among the 3227 cases where the reading was possible the antibody titer for the C.F.T. was, for the most part, 2-4 times, the highest being 16 times and over 32 times was not seen.
3) The mathematical analysis of antibody distribution and the individual aggregation of positive cases led us to the conclusion that the boundary of positive C.F.T. should be set on over 4 times. Over 2 times, however, could be regarded as positive, though not so accurate. The former is applicable to the new infections and the latter to the whole infections.
4) In the same way the boundary of neutralisation test was studied and the conclusion was reached that it lies between 1.0-1.7 of neutralisation logarithm. From the mutual relation of the two tests, the boundary of C. F. T. could be thus set on 2 times.
In this paper the theoretical ground for the fixation of boundary of positive C. F. T. and N.T. was given which may contribute to the investitation of latent infection of japanese encephalitis.

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Eighteen strains of coli group possessing the tyrosine-decomposing ferment were isolated from the feces of Ekiri patients, from which no dysentery bacillus was recovered. Biochemical and immunological studies were carried out on these strains. The results were as follows.
1) The organisms were identified as E. coli (16 strains), Citrobacter (1 strain) and Klebsiella (1 strain).
2) These strains were classified after Kauffmann's schema; two strains of O-1 and one strain of O-17, O-22, O-25, O-26, O-106, O-118, O-122 and O-127, respectively. The remaining 3 strains could not be identified.
3) There were 14 strains with the Invic reactions typical for E. coli, the others being atypical ones. One strain proved to be an indol-negative and another a KCN-positive variant, though these two belonged immunologically to the same type, E. coli O-1. There were besides a lactose-negative (E. coli O-21) and an anerogenic variant (E. coli O-2).
4) These organisms were tested on the activity of decarboxylases of arginin, lysine, histidine, ornitine, tyrosine and glutamic acid. The ferments decompsing glutamic acid, lysine and arginin were found in 95.5%, 90.9% and 81.4%, respectively. There were relatively few strains demonstrating decarboxylases of histidine, tyrosine and ornitine.
5) There were only 3 strains with all 6 ferments, whereas decarboxylases of arginine, lysine and histidine were found in almost all strains (15/16).
6) Different colonies of a strain were tested on the activity of these ferments, which sometimes fluctuated to some extent.
7) A strain of E. coli which showed a wide range of decarboxylase activity produced. an amine-like substance in the bouillon culture. This substance was studied by the paperchromatography. The Rf value of this substance was similar to those of histidine, cataverine as well as putrescine, only it showed a higher toxicity to mice than toxic amines.

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