Effects of training on the hematological values were measured in growing male and female rats. In the condition of normal feeding, body weight in male rats increased gradually as days pass. The increase in the body weight of female rats became moderate after 50 days old. Hemoglobin concentration and hematocrit value in male rats, increased gradually in proportion to the growth of the body weight. But these values in female rats, did not increase as much as that in the male rats. Accordingly, after 70 days of age, sex difference became statistically significant in the hemoglobin and hematocrit value. From 30 days to 100 days of age, the plasma volume per 100 g body weight decreased gradually, and the plasma volume of a female rat was less than that of a male rat of the same body weight. During such a growing period, the rat fed on 20% casein diet or 5% casein diet were compelled to swim 1 hour per day. The concentration of hemoglobin in a training male rat fed on 20% casein diet and the plasma volume per 100 g body weight, were the same as that of the control male rat. But in the case of the training female rat fed on 20% casein diet, hemoglobin hematocrit, and blood volume, plasma volume, red cell volume and total hemoglobin per 100 g body weight were significantly higher than that of a control female rat. The concentration in hemoglobin and hematocrit value in the training male rat fed on 5% casein diet did not differ significantly from that of the control male rat fed on 5% casein diet, and hemoglobin and hematocrit in the both groups of rats (control and training) were significantly lower than that of the male rat fed on 20% casein diet. In the female rats fed on 5% casein diet, the hemoglobin and hematocrit values in both groups of rats (control and training) were the same, and they did not differ from that of the female control rats fed on 20% casein diet. The volume of blood, plasma, red cell and total hemoglobin in 100 g body weight of the control male and female rats fed on 5% casein diet was significantly smaller than that of the rat of the same body weight fed on 20% casein diet. These values in the training group did not differ from that of the rats of the same body weights fed on 20% casein diet, it was suggested that the lean body mass, red cell volume and total hemoglobin were increased by training even on the low protein diet.
Diurnal variations of the hepatic tyrosine aminotransferase (TAT) activity were studiedin rats treated with streptozotocin which induces hypoinsulinism resulting in diabetes. The rats had free access to a diet during one half of each day and were fasted during another half of the day in the entire period of the experiment. Clear diurnal variations of the TAT activity were observed in both normal and diabetic rats. The diurnal variations in the TAT activity were not primarily due to a light-dark cycle but in response to food intake : that is, the activity of TAT was high during the feeding period independently of a lightdark cycle. The amplitude of the diurnal variations was higher in diabetic rats than in normal rats. Vmax values for tyrosine of TAT remarkably increased during the feeding time in both normal and diabetic rats. In both groups Km values for tyrosine inclined to decrease during the feeding time. In particular, diabetic rats showed a slight but statistically significant decrease in the Km values of TAT during the feeding time. The overall evidence demonstrated that diurnal variations of the TAT activity were amplified in streptozotocin-induced diabetic rats and therefore suggests that insulin is not involved in developing the rhythmic rise in the TAT activity which results from food intake.