Specimens of the experimental cutaneous calcinosis in the rat induced by topidal calciphylaxis (Selye) were studied by histological, histochemical and
35S autoradiographic techniques and calcificationmechanism was discussed in this paper.
1) The present research yielded the following results in a dynamic process of calcification.The cutaneous calcinosis in the rat was induced within 1-3 days after sensitization with oraldihydrotachysterol (DHT) and the use of subcutaneous ferric chloride.In the early stage of calcinosis, acid mucopolysaccharides (abbr. AMPS) exhibiting histochemicalproperties of hyaluronic acid increased in the calcifying area or the adjacent to it, with highactivity of alkaline phosphatase, acid phosphatase and AT Pase and the higher calcinosis, the moreAMPS which would be mainly chondroitin sulfate increased in the calcifying araa.
2) In calcification mechanism, proposing some importance of the organic matrix which way be thenucleating center of hydroxyapatite crystallization, we suggest much of the role of AMPS (particularly hyaluronic acid) or AMPS-Protein complexes for the matrix formation. It would be rather secondary change that chondroitin sulfates appeared in the highly calcifiedarea.
3) From predescribed findings, the significance or role of alkaline phosphtase, acid phosphatase andATPase in calcification mechanisms was discussed.
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