Wounds were made experimentally on the back of rabbits by the extirpation of the skin approximate 10×15cm square. After 10 and 20 days, the granulation tissue were removed for biochemical analysis of its lipids content and for in vitro experiments of lipids synthesis by incorporation of acetate 1-¹⁴C with or without various cofactors.
In analysis, 20 day granulation contained cholesterols, neutral fat and phospholipids in high magnitude comparing 10 days, however, none of squalene could be found in granulation at both periods.
From the results of acetate 1-¹⁴C incorporation study, 10 day granulation showed higher synthetic activity on sterols, neutral fat and phospholipids than 20 days, especially acetate-¹⁴C into choresterols and phospholipids incorporated more rapidly than into neutral fat.
In attempting to interpret the influences of cofactors on the lipids synthesis, adding NADP, ATP, Coenzyme A and citrate to the incubation media the incorporation of acetate 1-¹⁴C was studied.
From this study, it was resulted that cholesterol synthenis was accelerated by Coeyzyme A plus ATP, citrate activated neutral fat synthesis and ATP influenced positively incorporation of acetate-1-¹⁴C into phospholipids. The natures of added cofactors was discussed in this paper.

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MBH cream (O/W type) was applied to normally pigmented areas remaining in 14 patients with extensive vitiligo of several years' duration, in order to inhibit pigmentation and to produce a relatively uniform coloring of the skin.
Bleaching was initiated with 5% of MBH cream, followed by 10% concentration in cases that there was no irritability. Temporary hyperpigmentation first occured in most patients, and bleaching effect was found more than one month later . Depigmentation gradually progressed, and completed more than three months after the commencement of the application. Successful uniform coloring of the skin was obtained in eight patients out of the 14, moderate uniform coloring in five, and difficulty of uniform coloring in one, because of MBH dermatitis . MBH dermatitis occured in three patients, in whom the application of MBH cream was discontinued for some time, and was applied again with 2% of MBH cream without any complication.
Histological studies were performed of vitiliginous and the surrounding areas of seven patients before the application of MBH cream, and at depigmented and the surrounding pigmented areas of four patients one to three months after the application of MBH cream. Initial diffuse hyperpigmented areas showed considerable liquefaction of the basal layer and incontinentia pigmenti. And in depigmented areas caused by the application of MBH cream, melanin granules of the basal layer were almost absent, dermal melanophages markedly decreased, and the epidermal melanocytes were not observed in dopa reaction. In osmium zinc iodide reaction, MBH-induced depigmented areas of early stage showed the increase of suprabasal dendritic cells in number, and completely depigmented areas showed a tendency to disappear the basal and suprabasal dendritie cells.

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