Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 17, Issue 3
Displaying 1-16 of 16 articles from this issue
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  • Studies on the Phage Protein. (IV)
    Amino Acid Components and 3 Constitutive Protein Fractions of P22 Phage Coat Protein.
    Michiko HORIKOSHI, Kiyoaki KAMIJO
    1971 Volume 17 Issue 3 Pages 427-431
    Published: October 10, 1971
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.17.427
    JOURNAL FREE ACCESS
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    Further investigations were performed on P22-protein (Coat protein of P22 phage). Amino acid components were estimated with P22-protein by the aid of automatic amino acid analyzer. Constitutive protein fractions were analyzed by polyacrylamide gel electrophoresis. Fractionation of the 3 components, found in PAGE electrophoresis, were successfully performed with DEAE-cellulsoe column chromatography. Results were summarized as follows. 1.P22-protein is composed of alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, leucine, isoleucine, lysine, methionine, phenyl-alanine, proline, serine, threonine, tyrosine and valine and the molar ratio of them were 9, 4, 16, 8, 11, 1, 6, 4, 4, 2, 3, 1, 6, 6, 2, and 9, per mg protein, respectively. 2.Three components were detected from P22-protein in polyacrylamide gel electrophoresis (PAGE). 3.Average relative migration of the 3 components in PAGE were 0.767, 1.199 and 1.426, respectively, when that of bovine serum albumin (BSA) was 1.000. 4.Three components were successfully separated by DEAE-cellulose column chromatography. 5.Elution was performed in following conditions, i.e., 0.5g of DEAE-cellulose was packed in 10mm (diameter) × 300mm (length) glass tube and 5ml of protein solution (2.6mg/ml) was loaded, then elution was carried out with 0.001M, 0.005M and 0.02M phosphate buffer pH. 8.8. The last buffer was added with NaCl to 0.14M. The flow rate of the buffers was 0.5ml per minute. 6.Migration of the 3 fractions, corresponding to each 100 ml of buffers, coincided well to those of the 3 components detected in PAGE.
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  • [title in Japanese]
    [in Japanese]
    1971 Volume 17 Issue 3 Pages 432-435
    Published: October 10, 1971
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.17.432
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    1971 Volume 17 Issue 3 Pages 436-441
    Published: October 10, 1971
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.17.436
    JOURNAL FREE ACCESS
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