Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 43, Issue 3
Displaying 1-23 of 23 articles from this issue
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Contents
  • Expression and physical association of FcαR and FcRγ chain in human mesangial cells
    YUSUKE SUZUKI
    1997 Volume 43 Issue 3 Pages 410-418
    Published: December 15, 1997
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.43.410
    JOURNAL FREE ACCESS
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    The characteristics of FcαR in human glomerular mesangial cells (MC) were investigated to assess the role of IgA/IgA-immune complex (IC) in IgA nephropathy and confirmed the gene expression of FcαR in cultured MC by RT-PCR and Southern blotting. From each cultured MC, two distinct cDNA products were amplified, subcloned and sequenced. The sequence of the major product around 900bp was completely identical to that of FcαR previously described. The smaller product had a 288bp deletion corresponding to Exon 2 encoding extracellular domain 2 of FcαR, suggesting that an alternative splicing form occurred in MC. Gene expression of FcRγ chain, a common signalling-subunit for Fc receptors, was also confirmed in this study. In the meantime, we established a mouse anti-human FcαR monoclonal antibody (AAR1) which recognizes the IgA-binding site of FcαR and clarified that AAR1 was capable of enhancing the mRNA expression of FcαR in MC. Furthermore, the author demonstrated the physical association of FcαR with the FcRγ chain by co-immunoprecipitation under stimulation with a high dose of the heat-aggregated IgA. It is suggested that polymeric IgA and/or IgA-IC can directly activate MC via FcαR associated with the γ chain.
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  • Effect of temperature on cell migration and proliferation during wound repair in cultured temperature-sensitive gastric epithelial cells
    KATSUYORI IIJIMA
    1997 Volume 43 Issue 3 Pages 419-425
    Published: December 15, 1997
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.43.419
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    Objective: The effect of temperature on cell migration and proliferation during wound repair process was assessed by a simple method using cultured gastric epithelial cell line. Materials: An immortalized gastric epithelial cell line, GSM06, derived from SV40 large T-antigen transgenic mouse stomach was used for this study. Methods: GSM06 cells were cultured for 72h with Daigo's T culture medium at 37°C. Cells formed a complete confluent cell sheet. After forming a confluent cell sheet, an artificial wound was created in the center of the cell sheet and the process of wound repair was monitored at different temperatures (33, 37, 39, 41°C) using a time lapse VTR system. In additional experiments, cells were incubated at 33°C for the initial 12h after wounding and then the incubation temperature was increased to 37°C or 39°C to assess the effect of changing the temperature during repair. Sequential changes in the size of the wound after wounding were measured by an image analyzer. The cell proliferation was also monitored sequentially using BrdU staining and the labeling index was calculated. Results: After wounding, cells located on the wound edge, formed lamellipodia and showed active ruffling movement until compete repair. The speed of repair was slowest in the 33°C group and highest in the 39°C group. Increasing the temperature from 33°C to 37°C or 39°C during repair accelerated the repair speed. Cell proliferation as assessed by BrdU staining was highest in 33°C group and lowest in the 39°C group. Conclusion: In this model, wound repair was completed by cell migration and proliferation as in the primary cultured gastric epithelial cell model. Cell migration was much more important in the 39°C group while cell proliferation was dominant in the 33°C group in the GSM06 cell model of wound repair. Therefore, physical stimulation due to temperature might play a role on gastric wound healing in vivo.
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  • Follow-up study of rotational acetabular osteotomy for the osteoarthritis of the hip joint with special attention to the remodeling of the grafted bone
    YOSHIAKI ITOH, MASAHIKO NOZAWA
    1997 Volume 43 Issue 3 Pages 426-436
    Published: December 15, 1997
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.43.426
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    Rotational acetabular osteotomy originally reported by Tagawa and Ninomiya has become a common procedure in Japan for dysplastic osteoarthritis of the hip joint. It enables us to reconstruct the joint with increased covering of the acetabulum over the femoral head and, at the same time, medialization of the laterally displaced femoral head. This study evaluated the clinical results of surgery and clarified the process of acetabular remodeling on roentgenography. Materials: Of 220 patients who underwent the osteotomy between 1986 and September 1996, 53 patients (60 joints) with more than 5 year follow-up data were selected for the study. There were 4 male (4 joints) and 49 female (56 joints) patients. Preoperatively, osteoarthritis was classified into 3 stages: pre- osteoarthritis (31 joints), early osteoarthritis (20 joints) and progressive (9 joints) stages. Methods: The patients were examined and the clinical results were assessed by the Japanese Orthopedic Association Hip-Rating System (JOA score). On roentgenography, the time course of changes in the osteotomized and bone-grafted acetabulum was divided into 1) the period when the grafted bone appeared to be 'accepted', 2) that when the margin of the graft became smooth, 3) that when the graft appeared to be homogeneous, and 4) that when the graft appeared solidly united to the host bone. Patient age at surgery, the stage of osteoarthritis, the CE angle (angle indicating lateralization of the femoral head) and radiological changes were correlated. For statistical analysis, t- test was used. Results: The average total JOA score (max. 100) improved from 72.6 (range 42-90) to 94.5 (range 76-100). The improvement in pain score (max. 40) was most remarkable : from 20.7 (range 0.30) to 36.9 (range 30-40). Greater clinical improvement was obtained in the pre- and early osteoarthritis groups than in patients with progressive osteoarthritis. The average preoperative CE angle of 1.8 degrees (range-35-18) increased to 37.3 (-5-50) at follow-up. However, in 2 patients, progression of osteoarthritis was noted on radiography. On average, remodeling of the acetabulum took 39.1 months to complete, but more time was needed when the patient was older and the CE angle smaller. Conclusions: Clinical improvement after rotational acetabular osteotomy was satisfactory in the majority of patients. Improvement was more prominent when the stage of the osteoarthritis was earlier and the CE angle was smaller. Remodeling of the treated acetabulum was completed within 3 years and 3 months, but the process was slower when the patient was older and the CE angle was smaller. Therefore, the timing of initiating postoperative weight-bearing should be individualized according to the severity of the disease and the age of the patient.
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  • Serum levels of procollagen III peptide and type IV collagen in patients with biliary atresia (preliminary study)
    KENTARO HORIKOSHI, HIROYUKI KOBAYASHI
    1997 Volume 43 Issue 3 Pages 437-443
    Published: December 15, 1997
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.43.437
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    Objective: N- terminal procollagen- III peptide (P-III-P) is a marker of fibrogenesis and inflammatory activity in the liver and type IV collagen is part of the basement membrane extracellular matrix. Serum P-III-P and Type IV collagen were measured in patients with BA to assess their prognostic value. Method: Twenty-seven postoperative BA patients and 15 normal controls were studied. Patients were grouped according to outcome. (Group I: good prognosis (n=11), Group II: intermediate prognosis (n=9), Group III: poor prognosis (n=7)). Serum P-III-P and Type IV collagen were determined by radioimmunoassays and one-step sandwich enzyme immunoassay. Results: In Group III, serum P-III-P (1.929±0.642U/ml) and Type IV collagen (365.71±68.52ng/ml) were significantly higher than those in Group II (P-III-P (1.356±0.213U/ml), Type IV collagen (228.89±47.29ng/ml)) and in Group I (P-III-P (1.027±0.212U/ml), Type IV collagen (170.91±48.05ng/ml)). However, there were no significant differences in serum P-III-P and Type IV collagen levels between Group I and the controls. Conclusion: We conclude that serum levels of P-III-P and Type IV collagen are good markers of prognosis and may be useful in evaluating the clinical status of patients with BA.
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  • Factors affecting knowledge of home welfare service information in the elderly
    HIDEKI SATOH
    1997 Volume 43 Issue 3 Pages 444-455
    Published: December 15, 1997
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.43.444
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    Objective: This study determined an index guideline for effectively and efficiently providing home welfare service information to the elderly. For this purpose, a survey was carried out among the elderly living at home to investigate whether the level of knowledge concerning 36 home welfare services for the elderly correlated with demographic, social and health factors. Materials and methods: The subjects were selected from 2,200 elderly citizens (over 65 years) living at home, in five districts of Hokkaido, by the stratified random sampling method. Valid responses were obtained from 1,611 people with an average age of 72.8 years old (57.5% men, 42.5% women). The items of the survey were gender, age, educational background, family composition, income, regional characteristics, health (self-rated health, history of treatment at medical institutions), and knowledge of home welfare service information for the elderly. For statistical analysis, we used descriptive statistics, factorial design and multiple classification analysis. Results: The results showed that the average number of home welfare services known by an individual was 15.5 (S.D.=9.92). Differences among individuals in the number of home welfare services known correlated with age, educational background, income, regional characteristics, and self-rated health. Conclusions: The results of this study suggest that information gathering among the elderly is poorest among passive individuals lacking much activity, but rather an activity based on the characteristics of demographic, social and health factors. Thus, future information on services for the elderly should be not only universally distributed but also include selected presentation of information adapted to individual characteristics.
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  • Factors affecting postoperative hypertrophy of free vascularized bone graft : Experimental animal study
    HITOSHI SUDA
    1997 Volume 43 Issue 3 Pages 456-463
    Published: December 15, 1997
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.43.456
    JOURNAL FREE ACCESS
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    Objectives : Free vascularized fibular grafts have been used to treat a large bony defect incurred by trauma, osteomyelitis, tumor or congenital conditions. When such a graft is used in children or in young adults, the grafted fibula has been shown to gradually increase in diameter postoperatively. This study investigated the pathomechanism of this hypertrophy. Materials and methods : Animal experiments were carried out using 73 mongrel puppies. In the left hindlimb, the mid portion of the tibial shaft measuring 3.5 cm in length was resected, then placed back to the original position. The animals were divided into four groups based on the blood supply to the graft. Group 1 : The periostium and periosteal blood supply were kept intact and the bone was fixed with an intramedullary nail after reaming. Group 2 : The periostium was kept intact, but blood supply to the periostium was ligated. Blood supply to the medullary canal was kept intact. Group 3 : The periostium was kept intact, but blood supply to both the periostium and the medullary canal was obliterated. Group 4 : The periostium was stripped away and blood supply to the medullary canal was obliterated. The animals were chronologically examined radiographically and histologically up to 7 months. Results : Hypertrophy of the grafted bone began to occur in postoperative week two in Group 1 animals, and the width gradually increased with time. However, there was hypertrophy demonstrated in the other groups. The bone was completely absorbed in Group 4. Histological findings of hypertrophy were characterized by subperiosteal fibrous ossification and a newly created abundant vascular network. Conclusions : Periosteal blood supply seemed to play an important role in hypertrophy of the grafted bone.
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