Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
Search
Advanced search
OR
Browse
Search
Volume 30, Issue 4
Displaying 1-16 of 16 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
Contents
  • A New Simplified Method to Measure Fatty Acid in Platelet Phospholipid from Diabetic Patients
    KAZUKO MATSUSHITA
    1984 Volume 30 Issue 4 Pages 459-470
    Published: 1984
    Released on J-STAGE: November 20, 2014
    DOIhttps://doi.org/10.14789/pjmj.30.459
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Phospholipids are the main components of the cell membrane, and fatty acids contained in them play an important role in performing cell functions. Therefore, if there were an easy method to measure cell membrane phospholipids and fatty acids, which are prostaglandin precursors, it would doubtless have various clinical applications. However, phospholipids are extracted and isolated by adding water as well as organic solvents. Moreover, at the time of TLC isolation, they are exposed to the air, thus resulting in the destruction of polyhydric unsaturated fatty acids because of peroxidation. In an attempt to prevent peroxidation, antioxidants are added. All the same, there is the need to use TLC in isolating those substances. Thus, the procedure for measuring phospholipid fatty acids is not only troublesome but also time-consuming; therefore, this procedure generally is not popular among clinicians. In an effort to make up for this shortcoming, the author has developed an easy method for isolating phospholipids without using water and exposing them to the air. By this method, the author measured the component ratios of platelet phospholipid fatty acids and their contents in 16 diabetics with a control group of six healthy subjects. This method was useful in collecting phospholipids, and the results of the measurement were as follows : In the diabetics, a significant increase was noted in the component ratios of lauric acid and arachidonic acid. From a quantitative viewpoint, too, there were significant increases in the concentrations of myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, and arachidonic acid. There were also significant correlations between the fatty acid component ratios of palmitoleic acid and arachidonic acid and their concentrations.
    View full abstract
    Download PDF (1417K)
  • Pre-and Postoperative Glucose Tolerance in Patient with Portal Hypertension
    YOSHINORI SATO
    1984 Volume 30 Issue 4 Pages 471-483
    Published: 1984
    Released on J-STAGE: November 20, 2014
    DOIhttps://doi.org/10.14789/pjmj.30.471
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    We perfomed blocking operations in 205 patients with portal hypertention from September 1979 to March 1983. One hundred and seventy-four cases were transthoracic esophageal transections (Sugiura procedure), 20 were Hassab's operations and 11 were transabdominal esophageal transections. Responses of the blood glucose, serum immunoreactive (IRI) and immunoreactive glucagon dl (IRG) to 100g oral glucose load insulin were studied in 104 patients with cirrhosis and 16 with idiopathic portal hypertention (IPH) before and after Sugiura procedure. 1) Glucose intolerance was recognized in 90 patients with cirrhosis (86%). These 90 cases were divided into two groups (linear type and parabolic type) by the blood glucose response to 100g OGTT (oral glucose tolerance test). The laboratory findings and results of the indocyanin green test (ICGR15, KICG, ICGRmax) were compared between these two groups, but no significant differences were found. 2) Hyperinsulinemia was observed in patients with cirrhosis. The IRI responses to the 100g OGTT were divided into three groups by the peak value of IRI (high group; IRI≥200μu/ml; medium group; 20%>IRI≥150μu/ml; low group; IRI<150μu/ml). The laboratory findings and ICGR15, KICG, ICGRmax, were also compared among these three groups. Significant differences were observed between the high and low group in total bilirubin (p>0.05), direct bilirubin (p<0.025), Kicc (p<0.05), and ICGR max (p<0.005). The study of the IRI response to the 100g OGTT was useful for the determination of operability in cases of portal hypertension. 3) The IRG response to the 100g OGTT was also studied. But there were no significant relations between IRG values and liver functsions. 4) After the Sugiura procedure, oxyhyperglycemia in the blood glucose response to the 100g OGTT and hypersecretion of IRI were observed. 5) The IRG response to 100g OGTT showed no remarkable change before and after the operation (Sugiura procedure).
    View full abstract
    Download PDF (1617K)
  • Double Filtration Plasma Exchange in the Treatment of Lupus Nephritis
    HIROSHI TSUDA
    1984 Volume 30 Issue 4 Pages 484-489
    Published: 1984
    Released on J-STAGE: November 20, 2014
    DOIhttps://doi.org/10.14789/pjmj.30.484
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Treatment with steroids and immunosupressants is cmmonly effective for lupus nephritis. However, there are some patients who resist these treatments and have a serious prognosis. We have been reporting the efficacy of thrapeutic plasma exchange by fresh frozen plasma (FFP) for lupus nephritis. Evidence of the risk of hepatitis or the shortage of FFP is preventing the progress of this therapeutic trial. To get around these problems, we have been studying the therapeutic effects of double filtration plasma exchnge (DFPE) for lupus nephritis. DFPE by using filters with two different pore sizes was applied for lupus nephritis. The patient's blood was drawn out and separated to psasma and corpuscles by a plasmaseparation filter (first filter). Then the separated plasma was passed through the second filter, which filtered out macromoleculer protein. The filtrated plasma was returned to the patient with the cells that had been separated by the first filter. The same volume of 5% albumin as drain out plasma was transfused to the patient. Ten patients with lupus nephritis were treated by DFPE. The efficacy evaluations were good in seven, moderate in two and poor in one. Proteinuria was improved in seven out of eight patients after four weeks. We conclude that the DFPE under the treatment with steroids and immunosupressants is effective for lupus nephritis. Further study will be needed to establish the indications for DFPE treatment in lupus nephritis.
    View full abstract
    Download PDF (805K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top