Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 48, Issue 1
Displaying 1-18 of 18 articles from this issue
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Contents
  • Correlation between retinal nerve fiber layer thickness and visual field loss in normal-tension glaucoma
    JUNJI ONO, TAIROU KIMURA
    2002 Volume 48 Issue 1 Pages 20-30
    Published: July 10, 2002
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.48.20
    JOURNAL FREE ACCESS
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    Purpose : To evaluate the correlation between retinal nerve fiber layer thickness and visual field loss in normal-tension glaucoma using nerve fiber analyzer GDxTM. Methods : NFLT of 69 eyes from 69 patients with NTG was analyzed using GDxTM. The mean deviation (MD) of measurements by analysis of Humphery automated perimeter and the stage of the disease classification using Greve's modified method of the Aulhorn Classificaiton measured the degree of visual field loss. The examinations are as follows; 1) Correlation between the measurements of 14 parameters and that of MD was evaluated. 2) Disease stage was classified using Greve's modified method of the Aulhorn classificaiton and compared 14 by parameters. 3) Multiple regression analysis for 12 parameters that were significant at stage I). Results : 1) Twelve parameters were correlated with progress on visual of field loss. 2) On multiple comparisons using Superior ratio and Max. modulation, there were significantly decreases between stage O and stage III to VI. 3) The multiple regression equation calculated was significant (p<0.01). The coefficient of determination was 70.9%. Conclusion : It was indicated that measurements using the fiber analyzer GDxTM would be able to detect the degree of visual field loss with a rate of 70.9%.
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  • Expression of integrin and cell-adhesion to extracellular matrix in the retinoblastoma cell line
    SHINJI NAKAMURA, NOBUYUKI EBIHARA, AKIRA MURAKAMI, NORIYOSHI SUEYOSHI
    2002 Volume 48 Issue 1 Pages 31-38
    Published: July 10, 2002
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.48.31
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    Objective : This study was designed to determine the profile of integrin expressions on the surface of Y79 retinoblastoma cell line in an attempt to understand the process of retinoblastoma cell invasion and metastasis. Next, we defined ECMs (extra-cellular matrices) that promote the adhesion of Y79 cells, and investigated the expression of this ECM in the tissues of optic nerve and retina. Materials : We used Y79 cells (retinoblastoma cell lines), a series of anti integrin Abs and some ECMs. Method : The expression of integrins on the cell surface was determined by flow cytometry. Y79 cells were tested for their ability to adhere to microtiter wells coated with ECMs. For immunohistochemistry, cryosections of optic nerve and retina were stained by the LSAB technique. Results : Y79 cells expressed α3, α4, α6, αvβ5 and β1 integrin subunits, and strongly adhered to laminin. Furthermore, the expression of laminin was observed in pial septum of optic nerve and retina. Conclusion : The finding thatY79 cells specifically and strongly adhered to laminin via integrins explains the mechanism underlying the invasion of retinoblastoma to the optic nerve and retina, which express as laminin.
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  • Intracameral injection of atelocollagen as an experimental glaucoma model in the albino rabbit
    TAKAYUKI KANDA, TAIRO KIMURA, NOBUYUKI EBIHARA, SHINJI NAKAMURA, YASUO ...
    2002 Volume 48 Issue 1 Pages 39-48
    Published: July 10, 2002
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.48.39
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    Objective : To create an experimental glaucoma model which is simple and highly reproducible and stable in the albino rabbit. Materials and methods : We injected approximately 0.1ml, 0.2ml, 0.25ml, or 0.3ml of 3% atelocollagen into the anterior chamber of one eye of adult albino rabbits with a 30-gauge needle after aqueous humor paracentesis (approximately 0.2ml). After 1, 7, 14, 28, and 56 days of intracameral injection, we measured the intraocular pressure (IOP) with a Tono-Pen XL tonometer, and examination of the anterior ocular segment was performed. Intracameral injection of atelocollagen was applied to one eye of rabbits with the fellow eye serving as a control. 56 days after intracameral injection, we sacrificed the rabbits and excised the eyes for histological analysis. Results : In all of the 7 eyes, which were injected with 0.1ml (n = 4) or 0.2ml (n = 4) of 3 % atelocollagen, elevated IOP (>20mmHg) was not maintained until 7 days after treatment. In all 7 eyes, which were injected with 0.25ml of 3% atelocollagen, elevated IOP (>20mmHg) was maintained until 56 days after treatment. Elevated IOP (>20mmHg) was also maintained until 56 days after treatment in 3 of 4 eyes that were injected with 0.3ml of 3% atelocollagen. In the remaining eye, however, the IOP decreased to < 20mmHg with in 2 days after the injection. Corneal neovascularization was observed in all atelocollagen-injected eyes, whereas the anterior ocular segment was subinflammatory. Histological analysis demonstrated that degenerated and vacuolated cells were observed in the retinal ganglion cell layer of the ocular hypertensive eye. A few TUNEL-positive cells were also seen. Conclusions : Creating an experimental glaucoma model by intracameral injection of 0.25ml of 3% atelocollagen was easy. This experimental model promises to be of value in further studies of glaucomatous optic nerve damage.
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  • Effectiveness of hydrophilic cyclosporin eye drops for penetrating keratoplasty on albino rabbits
    HIROYUKI KAWANO, KAZUTAKA ADACHI, TOSHIYUKI YOKOYAMA
    2002 Volume 48 Issue 1 Pages 49-55
    Published: July 10, 2002
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.48.49
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    Objective: This study investigated the optimal concentration of hydrophilic cyclosporin A (CYA) eye drop on minimal stimulus, maximal transmission and on graft survival after corneal transplantation in rabbits. Materials and Methods: Stimulant effects of 0.1% CYA eye drop solution in a new developed vehicle and that in α-cyclodextran (α-CD) were measured by the Draize method. The concentration of CYA in corneas after the administration of 0.05% CYA eye drop solution in the new vehicle and that in α-CD were compared. We evaluated corneal grafts after exchange penetrating keratoplasties among three groups. Group 1: rabbits were treated postoperatively with 0.05% CYA eye drop in the new vehicle administered 3 times a day for 50 days. Group 2: rabbits were treated with 0.01% CYA eye drop under the same protocol. Group 3 was the control group. Suture removals of the cornea and exchange skin grafting were carried out on the 14th post operative day in all groups. The grafted corneas were evaluated after 50 days and were scored for neovascularization, opacity and edema. Measurement and Results: There was remarkable stimulant effect detected in 0.1% CYA eye drop solution in new vehicle compared to that in α-CD. Higher concentrations of CYA were found in the cornea (without epithelium) after the administration of 0.05% CYA eye drop solution in new vehicle for 24 hours. The postoperative survival rates after 50 days were: Group 1 81.8% ; Group 2 54.5% ; Group 3 9.1%. A significant deference in graft survival was noted between Group 1 and Group 3, but not between Group 2 and Group 3. Conclusions: These results demonstrate that hydrophilic 0.05% CYA eye drop in new vehicle may be immunologically effective for preventing rejection after corneal transplantation.
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  • A clinical evaluation of various multifocal (bifocal) contact lenses
    KOZO TAKAHASHI, KAZUSHIGE SADO
    2002 Volume 48 Issue 1 Pages 56-65
    Published: July 10, 2002
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.48.56
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    Objective : Recently, various types of Bifocal Contact Lenses (BFCLs) have been developed. In this study, quantitative aspect such as visual acuity with corrected BFCLs and qualitative aspects such as contrast sensitivity were examined to assess whether they improve their daily of lives. Participants : One hundred twenty subjects (224 eyes), 17 males and 103 females, with BFCLs prescribed and followed by Juntendo Univesity Hospital, participated in this study between October 1997 up to January 2001. The average age is 51.7±8.5 years with a range from 40 to 64 years. Methods : Visual acuity near and at a distance and contrast sensitivity were examined using ordinal visual acuity charts and The Multivision Contrast Tester (MCT8000).(Vistec Inc, USA). In addition, qualitative aspects such as how often BFCLs were changed, how long they were worn in a day and how satisfied the patient was when interviewed. Measurement and Result : BFCLs were represcribed 1.46±0.45 times on average during the observation period. De spite poor contrast sensitivity at both middle and high spatial frequencies, Distance and near visual acuity with corrected BFCLs has, more or less, good results. Distance visual acuity is 0.93±0.07 on average, near visual acuity is 0.78±0.15. Conclusion : It is true that this study may indicate that BFCLs are more useful than expected during daily life but monofocal contact lenses or glasses are superior to BFCLs with regard to contrast sensitivity. Soft contact lenses with alternative vision design and Rigid Gas Permeable with alternative vision are recommended among the various BFCLs available, if contrast sensitivity is being primarily considered.
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