Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 37, Issue 2
Displaying 1-20 of 20 articles from this issue
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Contents
  • Localization of vasopressin mRNA in human brain by non-isotopic hybridization histochemistry
    JIRO OHWADA, IWAHIDE NOGUCHI, HEII ARAI, REIJI IIZUKA
    1991 Volume 37 Issue 2 Pages 183-187
    Published: July 20, 1991
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.37.183
    JOURNAL FREE ACCESS
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    The technique of in situ hybridization histochemistry (ISHH) has been developed but has not been applied to the human brain. ISHH should be used along with immunohistochemistry to determine the distributions of neuropeptide-containing neurons, to overcome the problems of immunohistochemistry, such as cross-reactivity of antiserum and frequent failure to stain perikarya of neurons. We applied an in situ hybridization technique with non-radioactive probes to detect arginin-vasopressin (AVP) mRNA-containing neurons in the human brain in order to confirm the distribution of the AVP-producing neurons in human brain. Positive neurons were observed in the hypothalamic region. The AVP mRNA-containing neurons in the supraoptic nucleus were magnocellular, those in the paraventricular nucleus were magnocellular and parvocellular, and those in the accessory nucleus were parvocellular. These findings were in good agreement with those obtained by immunohistochemical examinations.
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  • A trial of homologous blood conservation and a multivariate analysis of the determinant factors for coronary artery bypass grafting without homologous transfusion
    -Effectiveness of frozen autologous transfusion and proper blood order-
    MASAHIRO GOTO
    1991 Volume 37 Issue 2 Pages 188-196
    Published: July 20, 1991
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.37.188
    JOURNAL FREE ACCESS
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    The complications of homologous transfusion are serious problems in coronary artery bypass grafting (CABG). In order to reduce the amount of homologous blood transfusion, we use frozen autologous blood, non-blood priming of cardiopulmonary bypass and salvage transfusion of all blood in the oxygenator at the conclusion of bypass. However, in some cases homologous transfusion is necessary. A discriminant analysis was made to identify the factors that determine whether homologous blood may be transfused or not in CABG. We examined the effectiveness of autologous transfusion and preparation of the amount of homologous blood before the operation. Two hundred and eight consecutive patients undergoing primary coronary revasculalization between April, 1987 and July, 1989 were studied. I compared 145 patients who did not receive homologous blood (Group 1) and 63 patients who received homologous blood (Group 2) in their profiles and operative variables. There were some significant differences between the two groups. The patients in Group 1 were younger and had a smaller proportion of women, higher body weight, larger body surface area, higher preoperative Hb value, higher frequency of autologous blood collection, milder illness, shorter cardiopulmonary bypass time, smaller chest tube drainage and larger amount of autologous transfusion. Comparison of operative mortality and morbidity did not reveal any significant differences except the incidence of reoperation for bleeding. These variables were used in a discriminant analysis to identify the factor reflecting of the need for homologous transfusion after CABG. Chest tube drainage in the first 12 hours in ICU was the most contributing factor, followed by the amount of autologous transfusion, patient's body weight, cardiopulmonary bypass time, preoperative Hb value. We examined the maximum surgical blood order schedule (MSBOS) in CABG. A maximum order of 1000ml blood was sufficient in single CABG, 2000ml blood in double and triple CABG, 3000ml blood in _??_uadruple or more complex. These findings should help improve the efficiency of ordering blood before the operation.
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  • A B cell differentiation antigen Lp-3 belonging to an immunoglobulin superfamily and the significance of the expression on B cells responsible for anti-DNA antibody production in murine lupus
    JUN SHIOTA, TAKASHI OKADA, MASAAKI ABE, HIROYUKI NISHIMURA
    1991 Volume 37 Issue 2 Pages 197-207
    Published: July 20, 1991
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.37.197
    JOURNAL FREE ACCESS
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    We previously found a murine B cell differentiation antigen, Lp-3, which can detect certain but not all populations of activated B cells. In the present study, we found that the Lp-3 expressions on the B cells responsible for spontaneous IgM and IgG class-anti-DNA antibody synthesis in systemic lupus erythematosus (SLE) -prone NZB×NZW F1 mice are Lp-3- and Lp-3+, respectively, Using SDS-PAGE analysis, Lp-3 was found to be a single polypeptide chain with an intramolecular disulfide bond (s), having a molecular mass of 132kDa. Analysis of the amino acid sequence of peptide fragments showed that the molecule has a structure belonging to the immunoglobulin superfamily. These findings suggest that the Lp-3 is a receptor or receptor-associated molecule for a certain B cell activation or differentiation factor, and is related to the immunoglobulin class switch of anti-DNA antibodies from IgM to IgG, which is an event associated with the development of lupus nephritis.
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  • Expression pattern of Thy-1.1 antigen in transgenic mice
    MASANORI AWAJI, JU-ICHI SAITOH, SHINGO NOZAWA, MASAAKI ABE, HIROYUKI N ...
    1991 Volume 37 Issue 2 Pages 208-217
    Published: July 20, 1991
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.37.208
    JOURNAL FREE ACCESS
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    We established a line of transgenic mice carrying the exogenous mouse Thy-1.1 gene (8.2kb EcoRI genomic DNA fragment). In these mice, Thy-1.1 was expressd on thymocytes but not on peripheral T cells, presumably due to the lack of cis-acting elements on the microinjected genomic DNA. In the thymus, the transgenic Thy-1.1 was expressed on most of the CD3/TCR- thymocytes. However, the CD 4+ or CD8+ single positive (SP) thymocytes consisted of Thy-1.1+ and Thy-1.1- subsets, the former considered to be at a premature stage of terminal T cell differentiation. There was no difference in the amount of CD3/TCR complexes expressed on the two SP thymocyte subsets. In the double negative (DN) thymocytes, all the CD3+/TCR+ cells were Thy-1.1-. These results suggest that the maturation process of CD3+ DN thymocytes differs from that of SP thymocytes. The distribution of the Thy-1.1+ population among the CD 3+ thymocytes suggests that the transgenic Thy-1.1 gene expression can serve as a useful marker to examine the terminal maturation processes of CD3+ thymocytes.
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  • A clinicopathological study of multiple nodular hyperplasia (nodular regenerative hyperplasia) of the liver
    HIDETOSHI SHIOTSU
    1991 Volume 37 Issue 2 Pages 218-230
    Published: July 20, 1991
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.37.218
    JOURNAL FREE ACCESS
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    Ten cases of multiple nodular hyperplasia of the liver, seven autopsy cases and three surgical cases, were studied clinicopathologically. Clinical features were various, and portal hypertension was found in three surgical cases. Macroscopically, in six autopsy cases the distribution of nodules was diffuse and in one it was scattered. In one case of the six cases with diffuse distribution the nodules were found with numerous metastatic adenocarcinoma of ovarial cancer. Microscopically, nodules were composed of thickened hepatic cell cords consisting of hypertrophic cells. Expanded nodules compressed the surrounding liver parenchyma without fibrous capsules. Most of the nodules had a portal tract in the center, some had a few portal tracts or central vein. These individual microscopical features of the nodules coincide with those of nodular regenerative hyperplasia of the liver reported by Steiner. Morphometrically, in the nodules, hepatic cells showed abundant cytoplasm, and nuclei were relatively monotonous. Sinusoidal spaces were narrowed. The nuclear DNA content of the nodular hepatic cells resembled that of the surrounding parenchyma. In four autopsy cases and one surgical case portal fibrosis was seen. One of the autopsy cases had been treated with anti-convulsant drugs for a long term and there was portal fibrosis with partial fibrous bridgings. In the case with scattered nodularity, severe sclerotic change of the arteries was seen in the portal areas, and complete obstruction in some portal areas. The sclerotic change of the extrahepatic portal vein was more conspicious in the cases with portal fibrosis than in those without. The nodules are considered to be caused by reactive hypertrophy of hepatic cells induced by disturbunce of intrahepatic microcirculation and hepatotrophic factors such as some hormones and drugs. And cases with scattered nodularity may exists as nodular regenerative hyperplasia different from that reported by Steiner.
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