Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 49, Issue 4
Displaying 1-21 of 21 articles from this issue
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Contents
  • Antibiotic Refractory Infection up date
    -Evolution of MRSA and Juntendo COE Project-
    KEIICHI HIRAMATSU
    2004 Volume 49 Issue 4 Pages 408-414
    Published: January 30, 2004
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.49.408
    JOURNAL FREE ACCESS
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    MRSA has caused numerous casualties in hospitals worldwide since 1960. It tends to resist various antibiotics, and its extreme form was reported as a highly vancomycin-resistant MRSA in 2002. Further more, MRSA started to prevail outside hospitals as well in the early 1990s, culminating in four paediatric deaths in 1999, as reported by the CDC in the USA. Drug-resistant bacteria are no longer confined to the hospital, but tend to cause infections in healthy individuals upon invasion to their normal flora.
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  • [title in Japanese]
    SATOSHI HORI
    2004 Volume 49 Issue 4 Pages 415-421
    Published: January 30, 2004
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.49.415
    JOURNAL FREE ACCESS
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  • Prevention of medical accidents and nosocomial infection by use of information technology.
    HIROHISA SATOH
    2004 Volume 49 Issue 4 Pages 422-428
    Published: January 30, 2004
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.49.422
    JOURNAL FREE ACCESS
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    In the Japanese medical field, it is widely considered that information technology is insufficiently utilized. Risk control is an essential issue for all hospitals, and We have learned that nurses directly face the risks of medical accidents. Especially, medication and infection control should be considered more deeply in order to decrease medical accidents. Recent progress in information technology has contributed to better medical risk management. In this report, two risk control systems to reduce medical accidents are introduced. 1) A portable data administrator (PDA) equipped with a barcode reader to read patient wrist bands is able to access by intranet patient data and a drug ordering server through a wireless Local Area Network. In order to eliminate mistakes, the nurse can confirm the drugs to be administered by bar code. 2) An infection control system consisting of patient reporting, histological data management, statistical analysis, and bacterial and drug information assists in selecting optimum treatments which lead to customer satisfaction. The system suggests optimum antibiotics to doctors based on the epidemiological analysis of past patient data. Real time monitoring for nosocomical infection allows rapid warnings to prevent out-breaks. If human operators use the system as a tool, and double-check its warnirgs, we may be able to decrease the rate of medical accidents.
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  • Tensin expression and localization in human renal tissues
    MICHIFUMI YAMASHITA, YASUHIKO TOMINO
    2004 Volume 49 Issue 4 Pages 459-465
    Published: January 30, 2004
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.49.459
    JOURNAL FREE ACCESS
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    Objective : Tensin is a 215kDa, actin-related protein. It is reported that tensin is expressed in tubular epithelial cells in the mouse kidney. In this study, we investigated tensin expression and localization in human renal tissues. Methods : Tensin expression and localization in renal tissues and renal cells were investigated using immunochemistry, RT-PCR and immunoprecipitation. The localization of tensin, integrin α5 and fibronectin was examined. The interaction between tensin and other actin-related proteins was also determined. Results : Tensin was localized not only in tubular epithelial cells but also in mesangial cells and glomerular parietal epithelial cells. Tensin was colocalized with integrin α5 and fibronectin in the mesagnial area, but only with fibronectin in tubular epithelial cells and glomerular parietal epithelial cells. In the mesangial cells, tensin was expressed at the end of the actin stress fiber, and was involved in complexes with the stabilizing molecule vinculin and signaling molecule FAK. Conclusions : In human renal tissues, tensin was expressed and localized not only in the tubular epithelial cells but also in the mesangial cells and glomerular parietal epithelial cells. Tensin may play functional roles in mesangial cells through adhesion complexes with integrin α5, vinculin and FAK.
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  • Tensin is expressed in rat glomerular mesangial cells.
    HISATSUGU TAKAHARA, YASUHIKO TOMINO
    2004 Volume 49 Issue 4 Pages 466-474
    Published: January 30, 2004
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.49.466
    JOURNAL FREE ACCESS
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    Objective : Glomerular expression of tensin was immunohistochemically studied in normal rat kidneys to determine whether tensin might be related to specific binding in individual glomerular cells. Materials : Kidneys and cultured mesangial cells from Sprague-Dawlay rats were used. Methods : Frozen sections of paraformaldehyde perfusion fixed kidneys were examined immunohistochemically using anti-tensin antibody. Cultured mesangial cells were examined at mRNA and protein levels by RT-PCR and immunoprecipitation. Results : Immunofluorescence of normal rat kidneys displayed intense reactions for tensin along the basal aspects of proximal and distal tubular cells, and parietal epithelial cells of the Bowman's capsules. In glomeruli, positive reactions for tensin were detected only in the mesangial areas. Immuno-electron microscopy revealed positive reactions for tensin in the mesangial cell (MC) processes. RT-PCR and immunoprecipitation demonstrated mRNA and protein levels of tensin in the cultured rat MCs. Conclusions : It appears that tensin is closely related to mesangial cell attachment to the surrounding extracellular matrix, and may play a role in the basic structural stabilizing system of the glomerular capillary architecture together with the glomerular basement membrane. Furthermore, tensin can act as a marker of rat MCs since the expression of tensin was detected only in MCs in the glomeruli.
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  • GATE-16 interacting protein
    TOMOHITO NISHITANI, ISEI TANIDA, TAKASHI UENO
    2004 Volume 49 Issue 4 Pages 475-486
    Published: January 30, 2004
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.49.475
    JOURNAL FREE ACCESS
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    Objective : GATE-16, originally identified as a protein necessary for intra-Golgi transport, has also been shown to be involved in starvation-induced autophagy. In order to better understand the dual functions of GATE-16, we performed a systematic analysis of GATE-16-interacting proteins, using ESI-QTOF (Q-STAR) massspectrometry. Methods : FLAG-GATE-16 was overexpressed in HEK293 cells and immunoprecipitated with anti-FLAG-M2-agarose. The resultant precipitates were analyzed by SDS-PAGE and identified by silverstaining. Each polypeptide was analyzed using ESI-QTOF (Q-STAR) massspectrometry. Results : Two proteins (p120, p105) were specifically coimmunoprecipitated with FLAG-GATE-16. The two polypeptides were identified as importin β and GRP94, respectively. When either of the two polypeptides and FLAG-GATE-16 were coexpressed in HEK293 cells, FLAG-GATE 16 was coimmunoprecipitated with importin β or GRP94. Conclusions : Importin β plays an essential role in protein import from the cytoplasm to the nucleus, whereas GRP94 functions as an endoplasmic reticulum (ER) chaperon. The specific interaction between the two proteins and GATE-16 may indicate that GATE-16 plays a regulatory role in importin β-dependent nuclear import and the uptake of de novo synthesized GRP precursor into ER.
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  • The first case of living donor liver transplantation in Juntendo University Hospital.
    HIROYUKI SUGO, SEIJI KAWASAKI, MASAKI FUKASAWA, HIROYUKI IWASE, KUNIMI ...
    2004 Volume 49 Issue 4 Pages 487-491
    Published: January 30, 2004
    Released on J-STAGE: November 12, 2014
    DOIhttps://doi.org/10.14789/pjmj.49.487
    JOURNAL FREE ACCESS
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    Living donor liver transplantation (LDLT) has been recognized as an effective therapeutic method for end-stage liver disease in Japan. However, meticulous planning is needed to start a transplant program. We herein describe the first case of LDLT in Juntendo University Hospital. To start the transplant program, a comprehensive treatment manual for LDLT was written and adopted in our hospital. Then, based on this manual, numerous staff meetings were held in each unit, including nursing staff, operation theater personnel, the division of clinical laboratory, and others. The first recipient was a 1-year-old girl with biliary atresia. She had a history of Kasai procedure at 101 days after birth. Both preoperative imaging and blood biochemistry examinations showed liver failure due to liver cirrhosis. She underwent LDLT and received a left lateral segmental graft obtained from her mother. The operation time was 16h and intraoperative blood loss was 203g. Their postoperative courses were uneventful and the recipient was discharged 50 days after transplantation.
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