Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
Search
Advanced search
OR
Browse
Search
Volume 58, Issue 1
Displaying 1-20 of 20 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
Contents
  • Increased Expression of Oxidative Stress in Proliferated Vascular Smooth Muscle Cells in Early Stage of Human Restenosis After Coronary Angioplasty
    TETSUYA OIGAWA, HIROMASA SUZUKI, SATOSHI SUNAYAMA, SACHIO KAWAIl, HIRO ...
    2012 Volume 58 Issue 1 Pages 35-43
    Published: February 29, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.35
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    A large body of evidence indicates that oxidative stress has been implicated in the development of restenosis formation after angioplasty in experimental animal models and clinical studies. However, the mechanism by which cells remove oxidative stress as a mediator of restenosis formation in vascular response to balloon-injury remains unclear and data concerning human tissue is lacking. This study was designed to clarify the role of oxidative stress in the process of neointimal hyperplasia formation of human restenosis lesions after coronary angioplasty. We allocated 122 neointimal hyperplasias obtained by directional coronary atherectomy (DCA) from 115 consecutive patients with restenosis into four groups, according to the time interval until restenosis formation after angioplasty. Cellular and matrix components of neointimal hyperplasia were immunohistologically studied among the groups. To gauge proliferative activity of cellular components, active proliferating cells were identified using antibody to proliferating cell nuclear antigen (PCNA) and positive cells were determined by double-label immunocytochemistry with a-actin, CD-68, leukocyte common antigen, Factor VIII and XIII, respectively. To characterize the potential role of oxidative stress in the process of neointimal hyperplasia, we also assessed the effect of 4-hydroxy-2-nonenal (HNE), a component of oxidatively modified lipids, on cellular components of restenosis. Consequently, the subject materials for this study consisted of 7 specimens at 0 to 1 month, 42 at 2 to 3 months, 57 at 4 to 6 months and 16 at 7 to 12 months after angioplasty. Neointimal hyperplasia could be histologically categorized into four morphological types of neointimal vascular smooth muscle cells (VSMCs). Predominant granulative type with large round nuclei of VSMCs and inflammatory cells was observed in 4 (57.1%) of the 7 specimens resected at 0 to 1 month after angioplasty. Granulative and mildly edematous type with medium-sized round nuclei of VSMCs and matrix deposits was found in 18 (42.9%) of the 42 specimens resected at 2 to 3 months. Out of 57 specimens resected at 4 to 6 months, markedly myxoedematous type with stellate-shape nuclei of VSMCs and more profound matrix deposits was predominantly found in as many as 39 (68.4%) lesions than that in other phases. In contrast, at 7 to 12 months, finely collagenized type with atrophic nuclei of VSMCs was detected in 10 (62.5%) of 16 specimens. The mean PCNA (12.6±4.9%) index of VSMCs in the neointima at 0 to 1 month was significantly greater than that at 2 to 3 month (6.7±2.1%) and 4 to 6 month (0.4±0.2%) after angioplasty (p>0.001). Moreover, the large nuclei of VSMCs in the predominant granulative type were strongly positive for HNE immunostaining compared with those of stellate VSMCs in the late phase of myxoedematous type neointima (9.3% vs. 0.1%, p<0.001). In conclusion, these data suggest that oxidative stress could play a pivotal role in proliferation of synthetic VSMCs, which has potential to produce extracellular matrix in development of restenosis early after angioplasty.
    View full abstract
    Download PDF (1371K)
  • ARRHYTHMOGENIC ACTIVITY IN LEFT VENTRICLES OF DILATED CARDIOMYOPATHY (DCM) MODEL MICE
    -DIFFERENCE BETWEEN ADRENERGIC AND CHOLINERGIC EFFECTS-
    TAKESHI SUZUKI, MASAMI SUGIHARA, YUJI NAKAZATO, TAKASHI MURAYAMA, TAKA ...
    2012 Volume 58 Issue 1 Pages 44-48
    Published: February 29, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.44
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Objective : Dilated cardiomyopathy (DCM) is a disease that often leads to heart failure (HF) and lethal arrhythmia. Recently a mouse model of DCM was created by a mutation of cardiac troponin T that closely mimics human DCM. These mice appeared to die suddenly with a t1 2 of 70 days. In the present study, to explore arrhythmogenic mechanism of the DCM mice, we examined effects of isoproterenol (Iso) and acetylcholine (Ach) on DCM myocardium because sensitivity of DCM hearts to autonomic effects seems to change in vivo. Materials and Methods : A knock-in mouse lacking Lys-210 in its endogenous cardiac troponin T gene (Tnnt2 ΔK210) was used as the DCM model at 8 to 10 weeks of age. Left ventricular (LV) muscles were isolated from wild type (WT) and DCM model mice. Membrane potentials were optically determined with di-4-ANEPPS using a laser scanning confocal microscope during field stimulation. The gene expression levels of receptors for autonomic nervous system and related downstream proteins were evaluated using a real-time PCR assay. Results : In normal Krebs solution, WT LV developed action potentials only by electrical stimulation, whereas DCM LV showed spontaneous action potential in addition to stimulation-induced activity. When the β-agonist isoproterenol was applied to LV, both WT and DCM LV developed spontaneous activity, and their frequency was much more prominent in DCM LV. The spontaneous activity was suppressed by β1-blocker (atenolol) and Ach, and their suppressive effects were much weaker in DCM LV. Gene expression analysis revealed down-regulation of KAch channel and up-regulation of some cAMP related proteins, whereas no significant change was detected in adrenaline β1-receptor. Conclusions : In DCM model mice, the change in balance between adrenergic and cholinergic effects of autonomic nerves on myocardium may relate to the development of lethal arrhythmias.
    View full abstract
    Download PDF (1576K)
  • EFFECTS OF THE PORTFOLIO ASSESSMENT ON THE CLINICAL TRAINING OF MEDICAL RADIOLOGICAL TECHNOLOGISTS
    TOHRU NISHIZAWA, KAZUO KEMPE, TAKASHI DAMBARA
    2012 Volume 58 Issue 1 Pages 49-53
    Published: February 29, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.49
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Aim : We have studied the effectiveness of the portfolio assessment for students in a medical radiological technologist school. Subjects : A total of 73 students were enrolled in this study. Every student received the same educational program, but they were divided into two different assessment method groups. Thirty-five students received portfolio assessment, and 33 students received non-portfolio assessment. Methods : At the beginning of the semester, every student received the same explanation for clinical training and educational goals. For the portfolio assessment group, how the portfolio was used for the training period was explained at the beginning of the semester. After the clinical training, we compared the scores of specific behavioral objectives (SBO) of the training by instructors and the students. Multiple choice question type (MCQ) test was also given and compared. For the portfolio group, a questionnaire on the portfolio assessment was given at the end of this study. Results : The scores of the MCQ and SBO of the portfolio group were higher than those of the non-portfolio group. However, there were no significant differences in the scores of SBO by the instructors between the two groups. Most of the students were favorable toward the portfolio assessment. Conclusions : This study suggests that portfolio assessment is an effective method for the clinical training of medical radiological technologists.
    View full abstract
    Download PDF (1319K)
  • EFFECTIVE DISEASE SUPPRESSION BY INITIAL STEROID THERAPY FOR MIXED CONNECTIVE TISSUE DISEASE
    YUKO TAKAHASHI, AKIO MIMORI, FUMIO SEKIYA, RAN MATSUDAIRA, KEN YAMAJI, ...
    2012 Volume 58 Issue 1 Pages 54-58
    Published: February 29, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.54
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Objective : To estimate the suppressive effects of initial steroid therapy on subsequent disease flares and disease duration requiring maintenance steroid therapy in patients with mixed connective tissue disease (MCTD). Methods : Clinical records from the past 15 years at Juntendo Medical School Hospital were reviewed, and 87 MCTD patients were identified (observation; 1148 person-years). The patients were classified into one of three groups based on the initial daily doses of prednisolone (PSL) : [0] 0 mg, n = 16; [L] < 20 mg (median 10 mg/d), n = 32; and [H] ? 20 mg/d (median 36 mg/d), n = 39. The entire disease course of each patient was analyzed for disease flares, which were denoted as MCTD events. Systemic lupus erythematosus disease activity index (SLEDAI) was used for estimating the initial disease activities in the patients. Results : Means of SLEDI at presentation of MCTD were [0] 3.2 ± 2.1 (n = 16), [L] 4.8 ± 2.3 (n = 32), and [H] 4.3 ± 3.1 (n = 39), and there were no significant differences in these parameters among the three groups based on t-test. Frequencies of major organ involvement in MCTD, i.e., progressive interstitial pneumonia or myositis (IP/myositis), aseptic meningitis, and pulmonary hypertension were [0] 0/16, 0/16, 1/16; [L] 4/32, 3/32, 1/32; and [H] 9/39, 3/39, 2/39, respectively. Kaplan-Meier MCTD eventfree analysis showed that patients in the no-therapy group [0] showed frequent events (p = 0.018, log-rank test), and most patients in the treated groups were IP/myositis event free throughout the observation period (p = 0.033). Patients in group [0] required higher doses of steroid therapy when suffering the subsequent MCTD events compared with group [L] patients (PSL : 49.0 ± 12.4 mg, n = 10 vs 31.6 ± 2.9 mg, n = 5, p = 0.016). Frequency of MCTD events during the initial 3 disease years was not associated with the following relapse rate, and MCTD events were infrequent after the 3 disease years. Conclusions : The initial steroid therapy for MCTD may suppress the following MCTD events even at a low dose. Initial steroid therapy may be beneficial even for patients with mild MCTD.
    View full abstract
    Download PDF (1475K)
  • Evaluation of the Aortic Curvature in Patients with Aortic Valve Stenosis
    Differences Between Patients with Bicuspid and Those with Tricuspid Aortic Valves
    SHIORI KAWASAKI, SATOSHI MATSUSHITA, TOMOYUKI FUJITA, TERUMASA MORITA, ...
    2012 Volume 58 Issue 1 Pages 59-64
    Published: February 29, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.59
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Background: Bicuspid aortic valve (BAV) is associated with both aortic valve stenosis and aortic disease such as true aneurysm or Stanford type A dissection. Currently the indications for concomitant surgery for the ascending aorta among patients with aortic valve stenosis are decided based on the diameter of the aorta at the time of surgery. Recently the ratio of aortic curvature has been reported to be an important predictor of future aortic events. In this paper, we evaluated the relationship between the valve phenotype and the ratio of aortic curvature among patients who underwent surgery for aortic valve stenosis. Methods: Out of a total of 142 patients who received aortic valve surgery, 117 patients who were evaluated using two-dimensional computed tomography (2D-CT) or echocardiography were analyzed retrospectively. The patients were divided into BAV and tricuspid aortic valve (TAV) groups. The ratio of the curvature was calculated using axially-resolved methods (X-axis, Y-axis and square root of X2 and Y2;√X2+Y2). Results: Forty-two patients had BAV and 75 had TAV. The X-axis component was significantly greater in the BAV group (18.8 ± 5.1 mm vs 12.3 ± 4.6 mm for BAV vs TAV, respectively [p<0.0001]), although the Y-axis component was not significantly different. A subanalysis of BAV types (R-L type: right and non coronary cusps fusion or A-P type: right and left coronary cusps fusion) demonstrated that the Y-axis component was significantly greater for the A-P type than for the R-L type, although the X-axis components were not different. Conclusions: Patients with BAV, especially the A-P type, exhibit a greater alteration in the ratio of aortic curvature. The morphological displacement is an additional important factor that concerns the surgical strategy for the ascending aorta in BAV patients with aortic stenosis.
    View full abstract
    Download PDF (1290K)
  • Pulmonary Metastases from Carotid Body Paraganglioma
    A Case Report
    KANAKO OGURA, MAKOTO HISHII, KAZUHISA TAKAHASHI, ATSUKO MIYAJI, MICHIH ...
    2012 Volume 58 Issue 1 Pages 65-68
    Published: February 29, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.65
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    We herein report a case of right carotid body paraganglioma that gave rise to metastases in the pulmonary parenchyma in a 43-year-old female. She had undergone a resection of a carotid body paraganglioma in 2003, which had no apparent histological features of malignancy. In 2005, multiple pulmonary nodules were detected during a routine examination. After careful follow-up for five years, Computed Tomography (CT) guided needle biopsy was performed, which revealed that the pulmonary nodules were metastases from the carotid body paraganglioma. Long-term follow-up is recommended for paragangliomas, since metastasis or local recurrence has been difficult to predict accurately using only histological evidence.
    View full abstract
    Download PDF (1523K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top