Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 25, Issue 1
Displaying 1-12 of 12 articles from this issue
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Contents
  • The Study of Argentaffin Cells in Benign Cerulative Colon Diseases
    KASTUMOTO ICHIKAWA
    1979 Volume 25 Issue 1 Pages 20-35
    Published: March 10, 1979
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.25.20
    JOURNAL FREE ACCESS
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    A study was carried on the distribution and numbers of argentaffin cells in surgically resected and biopsyed out colon. The resected materials were 34 cases of colon and rectum cancer, 8 cases of colon diverticulosis, 9 cases of colon tuberculosis, 9 cases of simple ulcer, 4 cases of ulcerative colitis, 5 cases of Crohn's disease and the 13 biopsy material of ulcerative colitis. Argentaffin cells were counted as numbers per 100 intestinal crypts. The results obtained were as follows : There were no difference in numbers of argentaffin cells in the surrounding areas of colon tuberculosis, companing with normal distribution. However, in the lesions of colon tuberculosis, the marked reduction of argentaffin cells were observed. In the simple ulcer, numbers of the argentaffin cells around the ulcer were increased. Argentaffin cells were increased in slightly inflammatory part of the ulcerative colitis resected in surgery and in biopsy materials at remission state and early active stage of ulcerative colitis. On the other hand, argentaffin cells were decreased in numbers in the lesions of Crohn's disease. The charactaristic distribution of argentaffin cells among these diseases was investigated in their lesion and their surrounding areas. Conclusively, it is very useful for the differential diagnosis of these diseases on the standpoint of the characteristic distribution of the argentaffin cells in the lesions and the surrounding areas
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  • Effect of Vitamin A and 5-Fluorouacil on Cultured Chondrocyte of Chick Embryo and Teratogenicity Test with Rats
    MASANORI IWADARE, WAKAKO ENDO
    1979 Volume 25 Issue 1 Pages 36-43
    Published: March 10, 1979
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.25.36
    JOURNAL FREE ACCESS
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    It has been known that hypervitaminosis A and 5-fluorouracil induced various anomalies in many animals. In this experiment, Sprague-Dawley rats were treated with 50, 0001.U/body i.p. of vitamin A or with 50mg/kg p.o. of 5-fluorouracil during organogenesis. The fetuses were removed from the mothers by cesarean section on 21st day of gestation, and were examined for gross and skeletal malformations. The skeletal checks were carried out after clearing soft tissue with KOH and staining the bones with alizarin red S. Under the present experimental conditions, vitamin A produced various types of skeletal malformation at fore limb, cranial bone, ribs, sternebrae and others. On the other hand, primary culture of chick embryo chondrocyte were performed and examined the effect of vitamin A and 5-fluorouracil. The cells were plated with the concentration of 3 x 104 cells/plate and drug was added after two days. Cell counts was done at every third day and total acid mucopolysachharide in the chondrocyte culture was estimated by quantitative toluidine blue staining. With 5-fluor ouracil at I0-6M marked effect was not seen but at the dose of 1 unit/m/ of vitamin A, growth rate of cells was inhibited and number of cells with a fibroblast-like morphology was increased.
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  • Studies on the Carbohydrate Chains and Their Functions in Human Alkaline Phosphatase Molecule
    TSUGIKAZU KOMODA
    1979 Volume 25 Issue 1 Pages 44-59
    Published: March 10, 1979
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.25.44
    JOURNAL FREE ACCESS
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    The relationship between the structure and function of alkaline phosphatase isoenzymes is under investigation in a number of laboratories. The present study deals with the effects of glycosidase or protease digestion on the alkaline phosphatase isoenzymes. Changes in physicochemical properties, catalytic activity, affinity for various lectins and blood group antisera, carbohydrate composition and biological half-life were investigated. 1. Purified intestinal enzyme from adults was found to contain little sialic acid, whereas the fetal enzyme was found to be a sialoglycoprotein. Marked differences were observed with respect to the content of other sugars as well. Howeevr, the carbohydrate compositions of adult liver and fetal intestinal enzyme preparations were found to be similar. 2. Km Value of sialidase-treated hepatic enzyme was changed to a value near of the enzyme from intestine. But, pH dependence curves of the log Km value showed a marked difference between intestinal enzyme and hepatic one. 3. Desialylated hepatic enzyme was shown to be more heat labile and more sensitive to protease digestion in the presence of 0.5 % SDS than native hepatic enzyme. Helix contents of native and desialylated hepatic enzymes were calculated to be 39.0% and 30.8/ respectively, apparent molecular weights 175,000 and 168,000, respectively. 4. Intestinal enzyme preparations treated with α-mannosidase, exo-N-acetyl-D-glucosaminidase and endo-N-acetyl-D-glucosaminidase-D displayed a decrease in enzyme activity. Among these, the α-monnosidase treated enzyme activity showed the most clearly reduction. By this treatment the maximum of the α-mannosidase-treated intestinal enzyme activity was shifted from 40mM Mg2+ to 5 - 10mM Mg2+. 5. Concanavalin A caused a biphasic modification of human alkaline phoshatase activity. The first stimulatory phase occurred at 0 - 0.02, μM, and the second inhibitory phase did at above 0.02, μM. The Hill coefficient was calculated to be about 0.5 in the stimulatory phase and 1.0 in the inhibitory one. According to the electrophoretic studies of the interaction between intestinal enzyme and some lectins, its sugar chain would be similar to that of the circulating glycoprotein. 6. Papain-treated enzyme contained a hydrophobic and carbohydrate-rich peptide. 7. Purified alkaline phosphatases from adult human organ were associated with a sugar chain containing a blood group substance. 8. The half-life of human alkaline phosphatase was found to be 125 h from liver and 7.5 h from intestinal preparations, respectively. Desialylation reduced the half-life of human hepatic enzyme to 22-25 h, but any additional glycosidase digestion of the carbohydrate moiety restored the half-life to nearly that of the native enzyme. Human intestinal enzyme with desialylation plus degalactosylation or with desialylation plus defucosylation showed a shortening of the half-life to about 1.2 h.
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  • Clinical Studies of Tonsillen Bacteriology
    RINYA SUGITA
    1979 Volume 25 Issue 1 Pages 60-65
    Published: March 10, 1979
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.25.60
    JOURNAL FREE ACCESS
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    Twenty six Cases of acute tonsillitis and 23 cases of chronic tonsillitis were investigated becteriologically. Fifteen tonsillectomized cases were examined bacteriologically with the swabbing and resected materials. The resuls were as follows : 1) The f-streptococcus occupied 50 per cent of all strains obtained from the cases of acute tonsillitis, but only 16% in the chronic cases. 2) In comparison with microorganisms of intra- and extratonsils, there were no significant difference, statistically. These microorganisms were Staphylococcus aureus, β-Streptococcus, Haemophilus influenzae, α-Streptococcus, Neisseria, H. parainfluenzae and Veillonella. 3) The results of the throat culture was quite reliable for the identification of etiological. organisms.
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  • Angiography in Gynecology
    TAKAO HIRAIWA, MITSUO TOKUNAGA, TOSHIO KOIDE, HITOSHI KATAYAMA
    1979 Volume 25 Issue 1 Pages 66-74
    Published: March 10, 1979
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.25.66
    JOURNAL FREE ACCESS
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    Superselective angiography is required to demonstrate the vascular anatomy, and this is really true with pelvic vascular system, which is quite complex because of superimposition. In 1972, Mohri reported a new modification of the catheter tip moulding and some improvement of the catheter technique of the superselective angiography of the uterine artery. In this article we present 26 cases of the superselective angiography with same technique. The catheter is moulded with two curves in the distal portion, the one is gently curved at 0.8-1.3cm from the catheter tip and the other is sharply curved at 12-13cm from the catheter tip. These two curves are not in the same plane, but the gentle curve is moulded so as to direct anteriorly when used in the catheterization. With this technique we have got time-saving merit by the angiography and have demonstrated more details of each lesion. Proper differential diagnoses could be achieved in the most cases. Cases of myoma uteri, myoma uteri with pregnancy and hydrosalpinx are presented.
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