Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 58, Issue 3
Displaying 1-17 of 17 articles from this issue
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Contents
  • IN VITRO MODEL FOR REMODELING OF THE SPIRAL ARTERY BY TROPHOBLASTIC CELLS
    -FOR ELUCIDATING PATHOLOGY OF PREECLAMPSIA-
    TARO KOSHIISHI, KEN-ICHI NAKAHAMA, XIANGLAN LI, SATORU TAKEDA, IK ...
    2012 Volume 58 Issue 3 Pages 211-217
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.211
    JOURNAL FREE ACCESS
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    Objective : Poor placentation is known to cause preeclampsia. Poor placentation results from a disorder of spiral artery remodeling by trophoblastic cells during the first trimester: however, the reason for poor placentation is not clear. To elucidate the pathology of preeclampsia, we have created an in vitro spiral artery remodeling evaluation system, which can be easily visualized under the microscope. Materials, Methods and Results : In the present study, we created and used a glass substrate in which cell seeding and transfer were possible along with the designed pattern by the optical lithography method. We carried out seeding of normal human umbilical vein endothelial cells (HUVEC) to the glass substrate, in which the cell adhesion domain was designed by the pattern of three lines of HUVEC. Twenty-four hours after contacting the substrate with HUVEC on Matrigel®, which is an extra-cellular matrix, a tube-like structure was transferred to Matrigel® after removing the substrate. Then, trophoblast cell line (TCL) 1 cells were seeded on the Matrigel®. TCL1 cells migrated on the tube-like structure formed by HUVEC, and replacement with HUVEC was observed. Conclusions : We successfully created an in vitro spiral artery remodeling evaluation system. With this evaluation system, observation of trophoblastic cell migration and replacement to the tube-like structure consisting of HUVEC became easy. We propose that this systems could contribute to the elucidation of the pathology of poor placentation in a near in vivo experiment.
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  • DETECTION OF STREPTOCOCCUS AGALACTIAE (GBS) AND CAPSULAR TYPES IN PRENATAL VAGINAL SPECIMENS BY REAL
    TIME PCR AS PART OF A NEONATAL GBS PREVENTION STRATEGY
    YUKO IGARASHI, NAOKI MITSUHASHI
    2012 Volume 58 Issue 3 Pages 218-223
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.218
    JOURNAL FREE ACCESS
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    Background : Streptococcus agalactiae (GBS) is a causative pathogen of meningitis and sepsis in neonates. We utilized a realtime PCR method for rapid detection of GBS in prenatal vaginal specimens. Materials and Methods : Vaginal swab samples (n=500) were collected from pregnant women aged 15 to 45 yr at 37 gestation weeks in Juntendo University Shizuoka hospital between September 2008 and September 2009. Samples were immediately analyzed by real-time PCR in parallel with routine bacterial cultivation. The next day, capsular type of GBS colony grown on the blood agar plate was identified by real-time PCR. Results : Sensitivity of PCR for 3 reference GBS stains was 10 CFU/well at 32 threshold cycle (Ct). Time required for real-time PCR analysis was about 1.5 hours. Of all the tested samples, 20.2% were PCR positive. GBS positive rates by age group were 23.4%, 18.0%, 13.3%, and 11.1% in the 30s, 20s, 40s and 10s, respectively. Predominant capsular type of GBS was serotype Ib (19.7%), followed by serotypes V (18.2%), VI (16.7%), Ia (12.1%), III (12.1%), VIII (9.1%), and II (4.5%). The parenteral antibiotic piperacillin was administered for the GBS-positive pregnant women and resulted in no GBS infection in newborns. Conclusions : We demonstrated that the GBS-specific real-time PCR assay was a sensitive and rapid tool for detection in pregnant GBS carriers compared to routine culture test, which may lead to timely use of prophylactic intrapartum antibiotics.
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  • High D Allele Frequency of ACE I/D Gene Polymorphism in Familial Hypertension in Javanese Indonesian
    FREDIE IRIJANTO, HERJANTI RAHAJENG, SUTARSO KAMAJAYA, MOCH. SJA'BANI, ...
    2012 Volume 58 Issue 3 Pages 224-230
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.224
    JOURNAL FREE ACCESS
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    Hypertension is considered as a multifactorial trait resulting from a combination of environmental and genetic factors. There are many genetic polymorphisms associated with hypertension. Polymorphisms of the genes coded with components of the renin angiotensin aldosterone system (RAAS) may influence the pathogenesis of essential hypertension. Recent studies have shown that D allele and the association with higher prevalence of hypertension is still an issue of debate and not established. There was a wide ethnic variation in ACE gene polymorphism. The objective of the present study was to investigate ACE I/D gene 287 bp polymorphism among hypertensive subjects with and without a history of family hypertension in a Javanese population. A cross sectional study was conducted in a community-based study in the districts of Sleman-Yogyakarta. There were 12,073 subjects more than 17 years old and 7695 in the 30-59 age range. Subjects were grouped as normotension, pre-hypertension and hypertension. Stratified randomized sampling was conducted to select 82 subjects with familial hypertension and 74 with non-familial hypertension in this study. Genotypes of the ACE gene polymorphisms (II/ID/DD) were determined using polymerase chain reaction (PCR) at the Biochemistry Laboratory, Faculty of Medicine Gadjah Mada University, Yogyakarta, Indonesia. The results showed that genotypic frequencies in the familial group versus the non-familial were 42 (51.2%) vs 57 (77%) II, 36 (43.9%) vs 15 (20.3%) ID and 4 (5%) vs 2 (2.7%) DD, p=0.002. The allelic frequencies were 120 (73.2%) vs 129 (87.2%) I and 44 (26.8%) vs 19 (12.8%) D allele, p=0.002. The genotype frequency in men with familial hypertension was higher than that of the non-familial group 22 (47.9%) vs 8 (21.6%) ID, p=0.004. Frequencies of D allele ACE gene and DD/ID genotypes in familial hypertensive subjects were higher than in non-familial subjects. The D allele frequency in women in the familial hypertensive group was higher than that in the non-familial group.
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  • Risk Factors for Persistent Pain and Disability in Acute to Subacute Sciatica Caused by Lumbar Disk Herniation After Epidural Injections
    YOSHIHITO MORITA, MASAKO ISEKI, IKUHO YONEZAWA, DAISHI NAKAHARA, JUNTA ...
    2012 Volume 58 Issue 3 Pages 231-237
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.231
    JOURNAL FREE ACCESS
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    Purpose: Identifying prospective predictors of a poor treatment outcome in acute to subacute sciatica would be of great importance for clinical practice. This study aimed to determine what baseline factors are associated with short- to medium-term outcomes on acute to subacute sciatica caused by lumbar disk herniation (LDH) after epidural injections. Methods: Variables including demographic data, neurological and radiological examination, the visual analog scale (VAS), the Roland-Morris Disability Questionnaire (RMDQ), and the Spielberger State-Trait Anxiety Inventory from 48 LDH patients (25 males and 23 females between 20 and 60 years old) with acute to subacute sciatica before and 1, 3, and 6 months following epidural injections were measured. A poor outcome was defined as a VAS reduction of less than 50% or a RMDQ reduction of less than an important change. Results: In multiple logistic regression analysis, the presence of a high trait anxiety (OR=0.133, 95% CI;0.027-0.651) and motor disturbances (OR=3.517, 95% CI;1.098-11.267) were significantly associated with an increasing risk of poor outcome at the 1-month assessment. At the 3-month assessment, the presence of a high trait anxiety (OR=0.115, 95% CI;0.022-0.611) was the only prognostic factor. At the 6-month assessment, the presence of a high trait anxiety (OR=0.065, 95% CI;0.007-0.570) was the only significant prognostic factor.
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  • Effect of N-acetylcysteine on Malondyaldehyde Content After Iron Treatment in Chronic Kidney Disease Stage 5D Patients
    MOCHAMMAD THAHA, MOCHAMAD YUSUF, WIDODO, RAKHMAN ANTON, PUTRI N WENNY, ...
    2012 Volume 58 Issue 3 Pages 238-243
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.238
    JOURNAL FREE ACCESS
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    Objective: This study was performed to evaluate and assess the influence of N-acetylcysteine (NAC) on malondialdehyde (MDA) levels as a biomarker of oxidative stress in chronic kidney disease (CKD) stage 5D (hemodialyzed) patients after parenteral iron therapy. Materials and Methods: This was a randomized, open-label, parallel-group trial comparing oxidative stress induced by intravenous (IV) administration of 100 mg of iron sucrose (control group) with an additional IV administration of 5 g NAC. At hemodialysis (HD) sessions pre- and post-plasma MDA levels were measured. Eighteen patients were enrolled, including 9 as controls and 9 in the treatment group. Results: In the control group, plasma MDA level increased from 3.71μM to 13.89μM (p=0.008), whereas in the treatment group, the level increased from 4.48μM to 7.89μM (p=0.059). The difference between the groups was also significantly different (p=0.015). Conclusions: It appears that NAC has the ability to decrease MDA level as a biomarker of oxidative stress caused by iron treatment in CKD patients.
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  • OVERVIEW OF TELEPHONE CONSULTATION SERVICE ON DEMENTIA
    TSUNEYOSHI OTA, TOHRU OHNUMA, SHIGENARI KIHIRA, YURIKO INABA, YUKAKO T ...
    2012 Volume 58 Issue 3 Pages 244-247
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.244
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    The Department of Psychiatry, Juntendo University School of Medicine started a telephone consultation service on dementia, funded by the Foundation for Prevention of Dementia, 1 year ago. The contents of the consultation were summarized as : medication (53%), symptoms (22%), hydrocephalus (12.5%) and relationship with doctors (12.5%). The medical staff involved in the treatment and care of dementia provided appropriate information demonstrating empathy and positive regard toward the anxious caregivers.
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  • PERSPECTIVES OF THE PRESENT STATUS OF MEDICAL EDUCATION IN MEDICAL AND GRADUATE COURSES, AND POSTGRADUATE CLINICAL TRAINING
    -REPORT ON THE 2011TH ANNUAL WORKSHOP (NARITA WORKSHOP) ON MEDICAL EDUCATION IN MEDICAL (THE 36TH ANNUAL WORKSHOP) AND GRADUATE COURSES (THE 10TH ANNUAL WORKSHOP) AT JUNTENDO UNIVERSITY, SCHOOL OF MEDICINE-
    TAKASHI DAMBARA, ISAO NAGAOKA, MASAKO NISHIZUKA, TOSHIAKI SHIMIZU, KAZ ...
    2012 Volume 58 Issue 3 Pages 248-255
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.248
    JOURNAL FREE ACCESS
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    The 2011th annual workshop on medical education in Juntendo University (Narita Workshop) took place in Narita, on July 15-16, 2011. In total, 205 people attended the workshop, including 114 faculty members, 27 graduates, 28 residents, 24 medical students and 12 administrative staff members. In the workshop, participants were divided into two committees (the medical education and graduate education committees), and they discussed proposals and perspectives on the present status of medical education in medical and graduate courses, and postgraduate clinical training.
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  • A GIRL WITH VITAMIN B12-DEFICIENT MEGALOBLASTIC ANEMIA ATTRIBUTED TO ABSORBING DISTURBANCE FROM INFANCY
    AYAKO KAMATA, KAORU OBINATA, MITSUYOSHI SUZUKI, HIDENORI HARUNA, KEIJI ...
    2012 Volume 58 Issue 3 Pages 256-260
    Published: June 30, 2012
    Released on J-STAGE: November 11, 2014
    DOIhttps://doi.org/10.14789/pjmj.58.256
    JOURNAL FREE ACCESS
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    We report a girl with vitamin B12-deficient megaloblastic anemia and her 11-year clinical course. She had failed to thrive and had eaten an unbalanced diet from the age of 16 months. At the age of 23 months, macrocytic normochromic anemia, megaloblastic changes in the bone marrow, and low serum vitamin B12 level were found. Her anemia improved soon after parenteral administration of vitamin B12. Her eating habits and physical development also improved. However, after withdrawal of the supplemented, serum vitamin B12 levels decreased gradually and macrocytic anemia appeared again. The vitamin B12 deficiency was attributed to both intake and absorbing disturbance of vitamin B12 in this patient.
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