Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 19, Issue 1
Displaying 1-20 of 20 articles from this issue
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  • A NEW DISC ELECTROPHORETIC METHOD FOR BASIC PROTEINS BY USING ALKALINE BUFFER SYSTEM
    HIROMI KUBAGAWA, HIDEOKI OGAWA
    1973 Volume 19 Issue 1 Pages 79-83
    Published: March 10, 1973
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.19.79
    JOURNAL FREE ACCESS
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    This report describes a new method of disc electrophoresis for strongly basic proteins using arginine-acetate buffer at pH 9.0 as a tray buffer. The electrophoretic patterns of various basic proteins (i. e., HML, EWL, cytochrome C and histone) were studied and compared with acidic and alkaline conditions. As to lysozymes, the mixture of HML and EWL, clearly separated into two discs under the alkaline condition although the electrophoretic mobilities of both lysozymes under acidic condition were identical or not significantly different. Cytochrome C, which exhibited a main brown disc under acidic condition, divided into two discs with brown color due to ferric ion. In histone, 7 discs that were detected in acidic condition separated into at least 15 discs under the alkaline condition.
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