Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 38, Issue 2
Displaying 1-18 of 18 articles from this issue
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Contents
  • Clinical pharmacokinetic study of intrahepatic arterial Adriamycin (ADR) combined with plasma exchange
    TSUTOMU KANNO
    1992 Volume 38 Issue 2 Pages 202-209
    Published: August 20, 1992
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.38.202
    JOURNAL FREE ACCESS
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    Plasma exchange combined with intrahepatic arterial administration of Adriamycin (ADR) was evaluated in gastrointestinal cancer patients with liver metastasis in an attempt to reduce the systemic distribution of the drug. The changes with time of peripheral blood levels of ADR after intrahepatic arterial administration at a dose of 30mg was examined up to 120 min after administration. ADR showed a distribution phase to decrease rapidly until 30 min after administration as a turning point and gradually disappear thereafter. The peripheral blood level of ADR 120 min after administration was 0.021μg/ ml/ min. In the pharmacokinetic evaluation of ADR by the trapezoidal rule, the area under the curve (AUC) was 6.252μg /ml / min up to 30 min after administration and 4.226μg/ ml/ min from 30 min to 120 min. The overall AUC was 10.476μg/ ml/ min. The ratio of AUC in the latter period of time after administration to the overall AUC, was estimated at 40.3%. Plasma exchange was combined after the turning point, 30 min after administration, and the peripheral blood level of ADR was found to have been reduced to an undetectable level. AUC was 5.672μg/ ml/ min, 1.476μg/ml/ min and 7.148μg/ ml/ min for fhe former period, latter period and overall, respectively, with the ratio of the latter period AUC to overall AUC being 20.6 %, which indicates that ADR could be eliminated from peripheral blood by the combined plasma exchange.
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  • Effects of electric acupuncture therapy on chronic prostatitis
    SHUICHI KATAI, RYUICHI KITAGAWA
    1992 Volume 38 Issue 2 Pages 210-219
    Published: August 20, 1992
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.38.210
    JOURNAL FREE ACCESS
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    To improve circulation in the prostate and its periphery, low frequency electric acupuncture therapy (EAT) was conducted in 100 patients with chronic prostatitis (nonbacterial prostatitis and prostatodynia). The patients were investigated by urinalysis, digital examination, ultrasonography, psychological testing, self-evaluation of symptoms, etc. EAT improved both white blood cell (WBC) count in the expressed prostatic secretion (EPS) and tenderness upon digital examination, by 64.7% and 82.1%, respectively. Although the efficacy of treatment was significantly lower in patients who were positive for WBC in EPS than in those who were negative (p<0.05), it was effective in 70.6% of WBC-positive cases. Thus, EAT is effective against chronic prostatitis to some extent. There were no significant differences in efficacy rate between patients taking medication and those without medication. Moreover, EAT was especially effective in patients with chronic prostatitis in whom inflammatory findings, neurosis, and depression were not severe. According to the general evaluation of subjective and objective findings, the efficacy rate of EAT was 79.3%. These findings indicate that EAT is useful in cases of chronic prostatitis in which medication and conventional urological treatment have little or no effect.
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  • The new methods of purification of the rat brain dynamin and its biochemical and ultrastructural analyse of the interaction with microtubules
    KUNIMI MAEDA
    1992 Volume 38 Issue 2 Pages 220-228
    Published: August 20, 1992
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.38.220
    JOURNAL FREE ACCESS
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    Dynamin was discovered in calf brain tissue as a nucleotide-sensitive microtubule-binding protein with a molecular mass of 100,000. Cross-linked microtubules into bundles and generated force to slide microtubules in vitro. Therefore, it was thought to be a third mechanochemical enzyme, following MAP 1 C and kinesin. Cloning and sequencing of rat brain dynamin complementary DNA revealed that it contained the consensus sequences of GTP-binding proteins. Studies on dynamin have been difficult because only a small amount of dynamin could be obtained from the brain. I developed a new purification method of dynamin using a small amount of rat brain tissue. It was a highly efficient and very simple procedure, and enabled a further purification step to obtain pure dynamin. I measured both ATPase and GT Pase activities. In the presence of microtubules, ATPase activity was activated 4 times that in their absence (Km 1.0). GTPase activity was activated 170 times (Km 0.07). This suggests that dynamin plays a greater role as GTPase in vivo than as ATPase. The binding manner of Dynamin to micortubules using a quick-freeze deep-etching technique, revealed that dynamin tightly binds to microtubules and decorates the surface of microtubule helically.
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  • Effect of gene linked to T cell receptor α chain gene complex of NZW mice on autoimmunity in NZB×NZW F1 mice
    SHINGO NOZAWA, KATSUTOSHI TOKUSHIGE, HIROYUKI NISHIMURA, SACHIKO HIROS ...
    1992 Volume 38 Issue 2 Pages 229-238
    Published: August 20, 1992
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.38.229
    JOURNAL FREE ACCESS
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    In the SLE-prone NZB × NZW F1 (NZB/WF1 mice), the unique T cell receptor β chain gene originating from NZW mice is known to contribute to the accelerated autoimmune disorders in association with the heterozygosity of H-2d/H-2z haplotype. To determine the possible role of TCR α chain gene in the autoimmune disease of NZB/W F1 mice, we examined the relation between the presence of TCR α chain gene of NZW mice and the severity of autoimmune manifestation in 69NZB /W F1 × NZB backcrossed mice. Although the TCR α chain gene of NZW mice alone is not related to the accelerated autoimmune disease, the presence of this gene resulted in a significantly high serum level of IgG 2 and-ds DNA autoantibodies and IgG anti-histone autoantibodies in association with other genetic factors such as the TCR β chain gene complex of NZW mice or H-2d/H-2Z heterozygosity. These findings suggestd that T cells expressing a particular combination of a and β chain genes both originating from NZW mice recognize the pathogenic antigen of F1 unique class II molecules and accelerate the autoimmune disease in NZB/W F1 mice.
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  • Effect of major histocompatibility complex on clonal proliferation of CD 5+B cells.
    FUMIAKI TAKIURA, TAKASHI OKADA, KATSUTOSHI TOKUSHIGE, SACHIKO HIROSE
    1992 Volume 38 Issue 2 Pages 239-248
    Published: August 20, 1992
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.38.239
    JOURNAL FREE ACCESS
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    Using H-2-congenic NZB, NZW and NZB/W F1 mice, we studied the effect of H-2 haplotype on the frequencies of CD 5+ B cells in the spleen and peritoneal cavity. We found that in the spleen of all the congenic strains with H-2Z/H-2Z haplotype, there were much higher frequencies of CD 5+ B cells than in the spleen of those with H-2d/H-2d or H-2d/H-2z haplotypes. These cells eventually proliferated in an oligoclonal or even monoclonal fashion, and B cell-chronic lymphocytic leukemia (B-CLL) developed in some cases. These findings suggest that a locus or cluster of loci closely linked to the H-2z complex of the N ZW strain may control the high frequency of CD 5+B cells in the spleen. By contrast, the CD 5+B cell frequency in the peritoneal cavity did not differ among the H2-congenic strains. Therefore, the frequencies of these cells in the peritoneal cavity and in the spleen appear to be under separate control. Flow cytometry and Southern blot analyses using an immunoglobulin gene J H proberevealed that in the H-2Z/H-2Z homozygotes, there was propagation of distinct clonal populations between the spleen and the peritoneal cavity. All our findings imply that certain different but related haplotypes of a major histocompatibility complex may predispose either to B-CLL or to autoimmune disease, in close relatives.
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