Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 24, Issue 1
Displaying 1-16 of 16 articles from this issue
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Contents
  • A Fundamental Study of Quantitative Cytology in Gastric Cancer Cells
    YUJI FUJII
    1978 Volume 24 Issue 1 Pages 32-48
    Published: March 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.32
    JOURNAL FREE ACCESS
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    While gastric cytology is useful for the diagnosis of carcinoma of the stomach, the present method is entirely dependent on the subjective judgement of the examiner. In this study, I have tried to approach to the quantification of cell findings for the cytologic diagnosis by scanning of the nuclei of the exfoliated cells with a microspectrophotometer. The scanning was made through a 1μ pinhole, recording the absorbance curve at the 538mμ wavelength, and the nuclear size, chromatin content, and chromatin pattern were analysed. The nuclear size was measured with a micrometer, the chromatin content was calculated as an integral of the absorbance curve, and the chromatin pattern was determined in the analysis of the waves. One handred and sixty normal cells and the same number of cancer cells were scanned and the following results were obtained : When the nuclear size only was used as a parameter, correct diagnosis was made in 97.8% (313/320 cells) and only misdiagnosis in 2.2% (7/320 cells); when the chromatin content was used, 98.7% (316/320 cells) was correct and 1.3% (4/320 cells) was wrong ; and when the chromatin pattern only was used, 98.1% (314/320 cells) was correct and 1.9% (6/320 cells) was wrong. When two parameters were combined, only one of 320 cells was misdiagnosed, and all the cells were correctly diagnosed in combination of the three parameters.
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  • A Study on Pericarditis
    FUMIYUKI TAKAHASHI
    1978 Volume 24 Issue 1 Pages 49-64
    Published: March 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.49
    JOURNAL FREE ACCESS
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    Pericardial heart disease is one of the more infrequent cardiac diseases, however, there is higher incidence in unrecognized pericarditis associated with collagen disease, uremia, or myocardial infarction. Since numerous hemodynamic studies of constrictive pericarditis and cardiac tamponade have appeared in the literature, it is surprising that many of the mechanisms responsible for the cardinal clinical features remain obscure. For evaluating the hemodynamic changes in acute cardiac compression, an experimental study was carried out with 23 open chest dogs under constant heart rate. The pressures were recorded from the left and/or right ventricle, right atrium, superior vena cava and intra-pericardial space, using a cathetertip manometer or water-filled catheter. Saline solution (37°C) was progressively infused into the intrapericardial space. Cardiac output was measured by the dye-dilution method and the peak dp/ dt of left ventricular pressure of postextrasystolic potentiation was examined as a parameter of myocardial contractility. The results of infusing the fluid into the pericardial space were proportional decrease in cardiac output, decrese in left ventricular pressure and decrease in the maximum velocity of left ventricular pressure rise. On further infusion of the fluid, these parameters decrased markedly. With the aspiration of the fluid from the pericardial cavity, the values returned to the control levels, before all fluid was withdrawn. This hysteresis was demonstrated during infusion and aspiration of the fluid from the pericardial cavity, and is considered to be due to the character of pericardium itself, judging from the pericardial pressure-volume relationship, using the sacrificed dogs. The mechanisms of the deterioration of hemodynamics during intrapericardial infusion was determined in the following order, 1) decrease in veno-atrial pressure gradient, 2) decreased srtroke volume, 3) impairment of atrial inflow during ventricular systole, and 4) domino effect of 1-3. In cardiac tamponade, the hypothesis on impairment of ventricular contactility caused by the reduction of coronary flow, was denied. It was reported that heart failure augmented postextrasystolic potentiation. In this experiment, however, there was no augmentation of postextrasystolic potentiation. So, conclusively, it may be derived that cardiac tamponade does not impair myocardial contractility.
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  • Effect of Erythropoietin on the Fetal Hemoglobin Synthesis in Nucleated Cells from Human Neonatal Cord Blood.
    JIRO KATAOKA
    1978 Volume 24 Issue 1 Pages 65-74
    Published: March 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.65
    JOURNAL FREE ACCESS
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    In order to investigate the effect of erythropoietin (ESF) on the synthesis of fetal hemoglobin (Hb F), nucleated cells separated from human cord blood in neonatus were cultured in the medium containing ESF. Radio-active iron as a marker of heme synthesis or tritiated leucine as a marker of globin synthesis was added to the cultured medium, respectively. The following results were obtained. a) Heme synthesis was accelerated by ESF until 72 hours. There was a close relationship between dose of the added ESF and heme synthesis. b) Hb F synthesis as well as adult hemoglobin (Hb A) synthesis were accelerated by ESF. These results support that ESF has a relation to fetal hemoglobin synthesis and suggest there might be erythroid progenitor cells, which respond to ESF, in cord blood. Fetal Hb and adult Hb were separated from hemoglobins of red cells in cord blood by Zade-Oppen's method and the nature of these hemoglobin as fetal hemoglobin as well as adult hemoglobin were examined by alkali-denaturation method (Singer's method). Then Zade-Oppen's method to separate hemoglobin was proved to be relaiable.
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