Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 24, Issue 4
Displaying 1-17 of 17 articles from this issue
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Contents
  • Cytogenetic Effects of N- (3', 4'-dimethxrycinnamoyl) Anthranilic Acid in Cultured Human Embryo Cells
    MASANORI IWADARE, WAKAKO ENDO, KIYOAKI KAMIJO
    1978 Volume 24 Issue 4 Pages 441-444
    Published: December 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.441
    JOURNAL FREE ACCESS
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    There is a growing concern that some of the drugs may produce genetic damage. Recently, many investigators have been reported that several drugs were added to the list of the chemicals capable of causing abnormalities in the chromosome of human somatic cells. The present study was undertaken to determine whether N- (3', 4'-dimethoxycinnamoyl) anthranilic acid (N-5'), a very effective anti-allergic agent, induced chromosome abnormalities in cultured human embryonic skin cells. As the results, there was no evidence for the increase of chromosome aberrations at a concentration of 200μg/ml or less. At 400μg/ml or higher, inhibition of cell growth was evident and these concentrations were unsuitable to observation of chromosome aberration in cultured embryonic skin cells.
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  • Clinicopathological Study of Endemic Goiter
    TZU-CHI SUNG
    1978 Volume 24 Issue 4 Pages 445-468
    Published: December 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.445
    JOURNAL FREE ACCESS
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    Five hundreds eighty-six patients with thyroid disease, living on the endemic area in Taiwan, were received thyroidectomy. Clinicopathological studies for 336 cases of endemic goiter of them were performed and summarized as follows : 1) In the total of 336 cases with endemic goiter, sex ratio was 1 : 7.6 (male 39 cases, female 297 cases) with predominance in female. Peak age incidence for female was 20 to 30-year-old, male was 30 to 40-year-old. 2) The more precise clinicopathololgical classification for endemic goiter are Stage 1- Hyperplastic phase, Stage 2-Enlarged phase and Stage 3-Nodular phase. 3) Of the total cases in this study;Stage 1 : Hyperplastic phase, 10 cases (3.0%) Stage 2 : Enlarged phase, 28 cases (8.3%) Stage 3 : Nodular phase, 88.7%; they were single nodule in 62 cases (18.5%) and multiple nodules in 236 cases (70.2%). 4) Thyroid goiter enlarged in correspondence to the clinicopathologic stage. In Stage 3-Nodular phase, there were cases having local compression sign, but no systemic symptoms and biochemical studies were in normal range. 5) Histopathologically, diffuse hyperplastic finding is characteristic for Stage 1, mild parenchymal enlargement of the thyroid gland may be seen, this is the “Hyperplastic Phase”. Stage 2, follicles are filled with colloid diffusely, scattering hyperplastic pictures and less mild involution may be seen in some place, but marked colloidal enlargement of the thyroid gland is characteristic, this is “Enlarged Phase”. Stage 3 is “Nodular Phase”, nodules were varing in size, single or multiple in number, histologically they show marked involutional changes. 6) In Stage 1, the lesions almost involved in both lobes; in Stage 2, equally in both lobes and single lobe; in Stage 3, more frequently in single lobe. The right lobe was larger than left lobe when the lesions involved in both lobes, but no difference in size involving in single lobe. 7) According to histopathological changes, clinical course and effects of iodine therapy, endemic goiter is progressing as stated above, and Stage 3 is the final phase. 8) There are 9.4% cases of Stage 3 associated with other throid disease. Secondary hyperthyroidism, 12 cases (4.03%), adenoma, 4 cases (1.34%), thyroid carcinoma, 12 cases (4.3%) these lesions are in close relation to the site of the nodule. 9) The effects of iodine therapy was predominantly in Stage 1, but non-effective in Stage 2 and Stage 3. Therefore, surgical treatment is considerable for Stage 2 and Stage 3. 10) Post-operative course of these 336 cases with endemic goiter after subtotal thyroidectomy were very well. Only 2 of them had mild hypothyroidism after the operation.
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  • A Novel Fluorometric Ultramicro Determination of Serum Leucine Aminopeptidase Using A Coumarine Derivative
    KOJI SAIFUKU
    1978 Volume 24 Issue 4 Pages 469-477
    Published: December 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.469
    JOURNAL FREE ACCESS
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    A simple and rapid rate assay of serum leucine aminopeptidase is described, using a novel fluorogenic substrate, 7-leucyl-4-methylcoumarinylamide. The reaction is initiated by adding 10μl of serum and the fluorescence development for 1 min. due to the 7-amino-4-methylcoumarin liberated at 37°C is followed directly on recorder. The proposed method is proved to be free from errors due to the adsorption of the substrate dye to serum albumin and to be applicable to hyperbilirubinemic sera by simple correction. The values obtained by this method showed a good correlation with those by the conventional method of Goldbarg and Rutenburg.
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  • Scanning Electron Microscopic Studies on Fallopian Tube, Human Uterine Endometrium and Placentas
    CHING-LANG CHEN, AKINORI SEIDA, TERUAKI OKUYAMA, HIROSHI FURUYA
    1978 Volume 24 Issue 4 Pages 478-484
    Published: December 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.478
    JOURNAL FREE ACCESS
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    The ultrastructure of the epithelium of the human fallopian tube and the uterine endometrium in normal cycles and of the human placental villi at every trimester of normal pregnancy were studied by scanning electron microscope. The materials for observations were obtained at the time of operation, artificial abortion and normal vaginal delivery. They were fixed immediately with glutar-aldehyde and then dried with the critical point in due course. Finally, ultrastructure of the tissues were obsered with scanning electron microscope (Hitachi MSM-II and S-550) with a magnification of up to ×100-×20000. The following results were obtained : 1. The Fallopian tube a. The most cells of the surface are ciliated or non-biliated, and peg cells were also observed sparsely. b. Both the ciliated and non-ciliated cells showed active apocrine secretion predominantly at the late secretory phase. c. Deciliation and transition to the non-ciliated cells (secretory cells) from ciliated cells following apocrine secretion were unable to be observed. d. Since it has been noted that estrogen stimulates growth in number of the ciliated cells and progesterone accelerates of the non-ciliated cells, our observation that both ciliated and non-ciliated cell show active apocrine secretion at the late secretory phase, suggest, presumably, that cells play an important part in providing the nutritional substances for the fertilized ovum under the influence of steroids. 2. Endometrium a. The surface of the endometrium is mainly covered with non-ciliated epithelial cells and sparsely with ciliated cells. b Cyclic changes of lining epithelial cells (non-ciliated) are markedly observed, namely, i) in shape and distribution of microvilli, ii) in arrangement of the cells which protrude toward uterine cavity, iii) in gap of the membranes of the cells and iv) in shape of the opening of the glands. 3. Human Placental villi During the first trimester of pregnancy, the placental villi have thick stems, a small number of branches and many sprouts. In the second trimester, the sprouts grow spreading many branches and at the terminal stage of pregnancy, branches of the villi become to show a treelike structure as a whole. Turflike microvilli without intertwining or sticking to each other are seen on the surface of placental villi throughout the period of pregnancy.
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  • Scanning and Transmision Electron Microscopic Studies on Human Term Placental Villi
    CHING-LANG CHEN, AKINORI SEIDA, TERUAKI OKUYAMA, HIROSHI FURUYA
    1978 Volume 24 Issue 4 Pages 485-491
    Published: December 10, 1978
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.24.485
    JOURNAL FREE ACCESS
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    The ultrastructure of the human term placental villi of normal pregnancy were studied by scanning and transmission electron microscope. The materials for observations were obtained at the time of cesarean section and normal vaginal delivery, fixed immediately with glutar-aldehyde and then dried with the critical point method in due course. Finally, ultrastructure of the tissues were observed by scanning electron microscope (Hitachi MSM-II and S-550) with a magnification of up to ×200-×20000, and by transmission electron microscope (Hitachi S-550) with a magnification of up to ×2160-×27000. The following results were obtained : I. Scanning electron microscope 1. Branches of the villi were observed to show a tree like fine structure as a whole. 2. On the surface of the placental villi turflike microvilli were seen without intertwining or sticking to each other. II. Transmission electron microscope 1. It was thought that many small vesicles close to the surface of the membrane of the syncytial cells indicate the occurrence of pinocytosis. 2. Many vesicles with granules in varying size were observed in the cytoplasm of the syncytial cells. 3. The mode of distribution of the microvilli on the surface of the villi were observed varying among the cells, someone few and someone absent. 4. The characteristics of the Langhans' cells were found to have fewer vesicles, lower total density and smaller RNP (ribonucleprotein) particles as compared with those of syncytial cells. 5. The cell membrane between the syncytium and the Langhans' cell was observed clearly. The desmosome on the cell membrane was sparsely. 6. The figure of transition to syncytial cells from Langhans' cells were unable to detected. 7. No definite cell membrane among the syncytium cells and a lot of syncytial knot in this layer were observed. 8. Thickness of the basement membrane, were measured to be about 300mu. On the basement membrane, it was found that more syncytium cells than the Langhans' cells are rested. 9. The stromal cell, bundles of collagen fiber and fine filament were seen in the layer of the stroma. 10. The endothelial cell, the basement membrane and the pericyte were observed in fetal capillary.
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