The author conducted feeding tests to determine the acute toxicity of DHA and sorbic acid for D.D. strain of mice and rabbits. In rabbits, hematological and histopathological studies were made to determine the toxicity. The following results were obtained : 1. The LD₅₀ of mice fed orally with DHA was found to be 1.72g/kg. and the 95% reliable range was 1.26 to 2.33g /kg. An accurate determination of the LD₅₀ for sorbic acid was not available because the tolerable dose was too great but it should be greater than 8.5g /kg. 2. In the daily feeding tests with DHA and sorbic acid, significant decrease in body weight, number of erythrocytes, specific gravity of the whole blood, hemoglobin, and hematocrit levels was seen only in the group fed 0.23g /kg. of DHA daily. However, no changes were found in the number of leucocytes, specific gravity of the plasma, blood sugar, resistance of the erythrocytes, urine values, K, Na, and Ca values of the serum. 3. Pathological examinations revealed toxic manifestations chiefly in the liver and spleen. The gastric mucosa showed a picture of catarrhal inflammation. No significant changes were found in the other organs.

View full abstract

A new method for testing the sugar fermentation reaction of yeasts were described. The new test medium was made of peptone : 0.5g, yeast extract : 0.3g, malt extract : 0.3g, sugar : 2.0g, agar : 1.5g, 1.2% B.T.B. solution : 3-4ml and distilled water : 100ml. The medium was autoclaved and made semi-slant like Kligller's medium. Sugar fermentation reaction of yeasts was very labile in the conventional test, but by this medium it was remarkablly stable. And moreover, many of the so called non-fermenters were found to be a latent fermenters, i. e., these cases were found to be 81.25% in glucose, 71.81% in maltose, 81.07% in galactose and 69.60% in sucrose.

View full abstract

The author conducted Some basic experiments on the refrigerated blood therapy, and obtained the following results. 1) One intravenous injection of homogeneous refrigerated blood in Small doses improves the recovery of rabbit's anamia caused by depletion. 2) The homogeneous refrigerated blood also improves the recovery of rabbits anamia caused by injections of chllargol. 3) A series of intraperitoneal jnjections of homogeneous refrigerated blood in small doses into the mouse exposed to a single whole-body rediation injury, decreased its mortality rate and increased its survival time. 4) The tissue respiration rate of homogenized mouse liver was incredsed by the addition of refrigerated blood extract. 5) The addition of refrigerated blood extract accelerates the fermentation of yeast and apozymase. 6) In using acceleration of yeast's fermentation, the source of the effective substance was investigated. It seemed that the effective substance was increased in the blood cells by refrigeration, and having increased it then moved to the serum.

View full abstract

1) From the X-ray shadow and electrocardiograph measurements of first rate sports atheletes in our couentry enlarged hearts were seen to parallel the amount of load and length of time in varsions fields of sports. Individnal differences can be seen fat not all enlargements of the heart can be explained by rational physiological dilatation alone ànd organic, irreversable changes cannot be denied. 2) Histological picture of heart muscles of mice after exercise loading (swimming) showed results different form that of Linzbach where these changes were explamed as a reasonable cend physiological adaptation. The results were rather those of phathological changes of severe hypertrophy. Although the results abtained in mice do not nesesserily apply to man it can be said that overtraining of young atheletes can be a potentially dangerous factor. 3) Post-mortum examination of atheletes with sudden death revealed underlying phathological changes and did not permit training to be considered as a related factors. The problem of sports medicine paesents many complex sociological factors with difference in the varions degrees of physical exersion, individual constitution, and tastes but it has become increasingly clear that atheletes must be given physical check-ups not only duning a ctive phase of their sports life but also after their termination. For further development of sports the problem of “sports heart” should be studied from tnese view-point.

View full abstract

Excretory pyelograms are calculated using planimeter in seventy six cases of stone hydronephrosis, and these cases are divided into five grades in regard to the size, form and funktion of the kidney. In twenty eight cases pyelograms before treatment are compared with those after taeatments, and in almost all cases these pyelograms returned to normal within one or two months. In stone hydronephrosis every effort must be made to preserve the kidney substance.

View full abstract

Pathohistological and statistic analysis was made in 430 cases of “so-called functional uterine bleeding” without organic changes, such as chronic endometritis, tuberculosis, abortion and tumors, etc. Following results were obtained. 1) Taking note of period of the curettage, pathohistological diagnosis of the endometrium by curettage was determined. 2) In 191 instances (44.5%), the lesions of endometrial hyperplasia were found. Endometrial hyperplasia was clasified into 2 types, tubulous glandular hyperplasia and cystic glandular hyperplasia.Tubulous glandular hyperplasia occured 145 times, and cystic glandular hyperplasia (',so-called Swisscheese pattern) 46 times. Endometrial hyperplasia occured by hyperesterinismus and difficiency of corpus luteum formation. 3) Appearently normal endometriums were found in 179 instances (42.8%). Normal proliferative phase occured 73 times. and secrtory phase 106 times. In above cases, mechanisum of bleeding refered to dysfunction of myometrium, not to drop of both ovarial hormons. 4) In 21 instances (4.8%), lesions of endometrial atrophy due to hypoesterinisums were found. Non-functional or aplastic endometrium refered to a passing hypoesterinisums, and true endmetrial atrophy refered to senile ovarial collapse. 5) In 14 cases, lesions of irregular shedding of the endometrium were found. Irregular shedding is a phenomenon of the prolonged menstrual shedding. The above mentioned phenomenon refered to prolonged reaction of progesteron. 6) Irregular ripening of the endometrium is same as so-called the mixed endmetrium. In 25 cases (5.9%), the lesions of irregular ripening were found. This phenomenon is due to unbalace of both ovarial hormons, and that concering to irregula shedding. As a result of the analysis, cause of the functional uterine bleeding was analogized. to unbalance of ovarial hormons.

View full abstract

1. The effect of bilateral mammary artery ligation on coronary disease was discussed and the simplicity and the largesy benifical results were emphasized. 2. The ECG cnanges of 26 cases of coronary insufficiency and myocardial infarction placed under this surgical procedure was studied and a slight improvement was seen. 3. Those cases showing strong sclerotic changes in the mammary arteries during the operation did not show any improvements.

View full abstract

After a certain period following castration groups of mature female rabbits were injected aubcutaneously at their back with 15 mg of estrogen pellet 1, 2, 3, 4 and 9 times at intervals of every 2 weeks, killed 2 weeks after the final administration and divided into 5 groups of 2 to 4 rabbits each according to total doses of 15 to 135 mg and periods of 2 to 18 weeks administered to produce the experimental hyperestrongenism with progesteron effect intercepted. Meanwhile, in order to confirm effccts of castration at each period, pathological, anatomical and histological examination were conducted chiefly on their uterus, mammary gland, pituitary body, thyroid gland, suprarenal gland, kidney and liver together with those of 5 control groups of 2 castrated animals each. Experimental results were classified roughly into two categories according to dose of estrogen and period administered. (1) In groups administered with 15 to 45 mg of estrogen pellet for 2 to 6 weeks 2 weeks after castration the endometrium atrophined and thinned by castration indicated a remarkable glandular proliferation, and the muscularis mucosae thinned was also hyperthophied several times. Until 6 weeks after castration the mammary gland not different from non-functional and normal control proliferated like fibroadenia and acinar adenosis, and the abmornally proliferated focus of galactophorous glands was noted. Up to 6 weeks after castration appearance of acidophile celles instead of basophile cells became remarkable in the anterior hypophysis where the latter was to proliferate, chromophobic cells also developed and eflect of castration was inhibited by administration of estrogen. The cortex became slightly hypertropeied 2 to 4 weeks after castration. Four weeks later fat decreased in the fascicular Layer of suprarenal cortex, but a large amount of fat was still encountered in the coniform layer of suprarenal cortex. However, continual decrease of fat was found in the fascicular Layer, and cells were hypertrophied from the depth to the reticular layer. The thyroid gland was reduced in function, was concentrated in colloid, and indicated large follicles due to castration. But it was weakened in the colloid, indicated small follicles and was enhanced in function due to administration of estrogen. Extending over prolonged period after castration binuclear cells developed in atrophied liver, while central vein and venous sinus were congested and enlarged. Estrogen had no effect on the spleen where there was noted no remarkable cnange after castration. Degenerated epithelium of the principal part of the nephritic duct, which became distinct 2 to 4 weeks after castration, was recoverd by administration of estrogen. (2) In groups administered with 60 to 135 mg of estrogen pellet for 8 to 18 weeks after castration the endometrium was markedly infiltrated with leukocytes showing the necroosis or the necrobiosis. The infiltra tion extended into the muscularis mucosae, and vein and cepillara of the uterine vagina were markedly congested and enlarged, part of them indicating the thrombosis. However, the residual muscularis mucosae was rather proliferative, and in groups administered with 135 mg of the medicine for 8 weeks the fibroma was formed below the serous membrane. In the mammary glands where rami of the galactophorous ducts temporarily exteded and the epithelium proliferated, lobules collapsed completely and large cystic mastopathy seemed to appear. In the anterior hypophysis surrounded by the stroma acidophile cells decreased and collapsed, while basophile cells diminished markedly and chromophobic cells increased remarkably instead. Fat in the coniform layer of the suprarenal cortex disappeared entirely, but a considerable amount of fat was noted in the fascicular layer. Concentration, collapse and disappearance of cytoblasts were noted.

View full abstract

An experimental Stuay on the spinal cord injury. The auther made an experimental study with rabbits on the radiological as well as histological findings of the spinal cord injuries which were caused by a fall from 3.75 meter's height. As a result of this study the following points shoud be noted. (1) Even in the cases with negative X-ray findings the fractures of spinal bones were proved with the anatomic studies, (2) Tigrolysis which was found in the ganglion cells much higher than the point of bone lesion, to indicate that the clinical signs often observed in the points of spinal cord higher than the damaged bone could be caused by a direct trauma on the remote area. (3) As possible causes of the edema in the spinal cord, the rhexis bleeding found in the spinal cord, should be added. (4) Regemeration of nerve cells of the spipal cord was not detected in all of the cases. (5) In one of the cases some inflammatory change was observed in the spinal cord, which requires further elucidation. The above experiment was performed on the animal which fell from a place of 3.75 meter's height. Accordingly the further study schould be made by changing the height of the place.

View full abstract

Using practically constant currents the author has carried out the measurement of the utilization time of nociceptors in the human normal anterior teeth under physically sufficient and physiologically appropriate experimental conditions. The utilization time has been very long and fluctuated between 0.5 and 2.5 sec. Of course, this utilization time is the “practical utilization time” because of the slight diminution of the practically constant effective voltage in the time course of the current flow. Such diminution of the order of 1 : 1000 may be caused by the galvanic polarization of the skin and presumably the tooth. The author has compared his results with the Sakamoto's theory of electric excitation as well as the strength-duration relation of the nociceptor in the human anterior teeth which was carefully determined by Sakamoto and Matsumoto, and consequently insisted the Sakamoto's opinion that the nociceptor in the human tooth does not exhibit the accommodation.

View full abstract