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原稿種別: 付録等
1960 年19 巻 p.
318-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
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原稿種別: 付録等
1960 年19 巻 p.
318-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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原稿種別: 付録等
1960 年19 巻 p.
318-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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原稿種別: 付録等
1960 年19 巻 p.
319-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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清水
原稿種別: 本文
1960 年19 巻 p.
319-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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清水
原稿種別: 本文
1960 年19 巻 p.
320-
発行日: 1960年
公開日: 2017/12/22
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茅根 竜平
原稿種別: 本文
1960 年19 巻 p.
321-328
発行日: 1960年
公開日: 2017/12/22
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The author has studied, histologically, the effect of vitamin D on the ovary and the uterus of albino rat, and reached to the following conclusions. Deficiency of vitamin D affects the follicular tissues of ovaries and especially the regressive degeneration of the growing follicles, which prohibits the ovulation, is very remarkable. Along with these changes in the ovaries, the uterus becomes atrophic. But these changes are recovered by an administration of vitamin D almost to normal condition. On the other hand, the animals, which has been given small amount of vitamin D, reveal the acceleration of the follicular development and the enlargement of uterus. These facts show that vitamin D has enhanced the functions of the sexual organs. If the animals are given a large amount of vitamin D over a long period of time, it will enhance the sexual function temporarily, but then the ovaries and the uteri will become atrophic and will finally lose their function completely.
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片山 喬
原稿種別: 本文
1960 年19 巻 p.
328-347
発行日: 1960年
公開日: 2017/12/22
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Experiments carried out in toads by the kidney irrigation method showed that thiamine was excreted mainly through the glomeruli. Histochemical investigations made on thiamine distribution in toad kidney tissue revealed that thiamine was secreted and reabsorbed by the tubules. Following the loading of thiamine, the vitamin excreted in urine was determined every 2 ot 3 minutes, and the thiamine loaded was shown to be excreted rapidly in urine. Similar experiments were carried out with cocarboxylase and thiamine propyldisulfide and the results were compared with the ones obtained with thiamine. Thiamine loading (20 mg) and phenolsulfonphthalein tests made simultaneously showed that there was a correlation between the excretions of phenolsulfonphthalein and thiamine. Tests made in rabbits with kidney disturbance developed experimentally with mercuric chloride showed that the excretion of thiamine nearly paralleled that of phenolsulfonphthalein. The excretion of thiamine in urine in relation to kidney function is discussed based on these findings.
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清水
原稿種別: 本文
1960 年19 巻 p.
347-
発行日: 1960年
公開日: 2017/12/22
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清水
原稿種別: 本文
1960 年19 巻 p.
348-
発行日: 1960年
公開日: 2017/12/22
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高田
原稿種別: 本文
1960 年19 巻 p.
348-
発行日: 1960年
公開日: 2017/12/22
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山田 晃
原稿種別: 本文
1960 年19 巻 p.
349-352
発行日: 1960年
公開日: 2017/12/22
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The accelerating effect of food colors of phthalein series on urinary thiamine excretion in rats was studied. The administration of eosin, erythrosin, and uranin for 2 weeks in a concentration of 1.44×10^<-2> moles resulted in increase of urinary thiamine as it was the case with phloxine reported in the previous paper, especially uranin showed a marked increase compared with the other two in the excretion percentage. The cause of this finding seems to be the effect of phthalein itself rather than halogen contained in phthalein compounds.
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藤田 秋治, 川口 露子, 森元 博久
原稿種別: 本文
1960 年19 巻 p.
352-357
発行日: 1960年
公開日: 2017/12/22
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Fractional determination of the stereoisomers of β-carotene described by Bickoff was tested and the improved procedure was described. Using hydrated lime as an absorbent and p-cresyl methyl ether in petroleum benzine as a developing solvent mixture, β-carotene was fractionated into three stereoisomers, i. e., neo-β-carotene B(B), neo-β-carotene U (U) and all-trans-β-carotene (T). Since α-carotene appears in the fraction of B, it must preliminarily be separated from β-carotene fraction by the first lime chromatography using petroleum benzine as a developing solvent. The ratio of the isomers was not affected by the duration of saponification. The distribution of the stereoisomers of β-carotene in a number of foodstuffs used for carotene source was determined and given in a table. In each case, T was 80-9O%, whereas each of B and U was below 10%. No other isomers were found. The ratios of the isomers in some fresh tissues were about the same as those of the dehydrated ones.
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小野 忠義
原稿種別: 本文
1960 年19 巻 p.
357-364
発行日: 1960年
公開日: 2017/12/22
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The effect on the thiaminase production by Clostridium thiaminolyticum (D-9) was tested with the media S-containing substaces. Addition of 10^<-2>M L-cysteine or DL-homocysteine was found to promote the thiaminase production, whereas the disulfide forms of the compounds were found to be inhibitory. Sodium thioglycolate and L-methionine were without effect, but BAL and glutathione were extremely inhibitory. The effects of these SH-compounds on thiaminase activity were found to be somewhat promoting. The effects of thiamine and its derivatives on the thiaminase production were promoting in the media containing cysteine (10^<-2>M) but were inhibitory in the media free from cysteine. Anti-thiamine substances inhibited thiaminase production.
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西田 実
原稿種別: 本文
1960 年19 巻 p.
364-367
発行日: 1960年
公開日: 2017/12/22
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Errors of determination in our previously reported chemical method of thioctic acid were induced from the following factors : (1) The time elapsed before reading the ultraviolet absorbancy after mixing dihydrothioctic acid and p-chloromercuribenzoate. (2) The change of pH in the reaction mixture. Changes of thioctic acid level in the blood were compared, after administering thioctic acid to rabbits by various methods.
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清水
原稿種別: 本文
1960 年19 巻 p.
367-
発行日: 1960年
公開日: 2017/12/22
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川崎 近太郎, 富田 勲
原稿種別: 本文
1960 年19 巻 p.
368-372
発行日: 1960年
公開日: 2017/12/22
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Cyanogen bromide and NaOH were applied to the solution of thiamine sulfuric acid ester (I) to form quantitatively thiochrome sulfuric acid ester (V) which is water-soluble, not extracted by isobutanol. The intensity of its fluorescence was proportional to the concentration between 1-10 μg of (I) and fairly stable in the alkaline solution. At the presence of free thiamine, the separate determination was enabled by shaking the reaction-mixture with isobutanol ; the isobutanol layer contained thiochrome while the water layer kept (V) remaining.
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川崎 近太郎, 富田 勲
原稿種別: 本文
1960 年19 巻 p.
372-376
発行日: 1960年
公開日: 2017/12/22
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On determining thiamine sulfuric acid ester, even when a purified sample of it was used, by cyanogen bromide method, an isobutanol layer has always shown a weak fluorescence of about one-tenth intensity compared to a water layer. The water layer was repeatedly shaken with isobutanol and 5〜7% of its fluorescence was found to be lost at each extraction while isobutanol got extracted the same proportion of fluorescence. Moreover both the isobutanol and water layers contained only thiochrome sulfuric acid ester which was detected by paper partition chromatography. The equal mixture of thiamine and its sulfuric acid ester was estimated by this method. The thiamine value found was 9-10% higher than the expected one, while the thiamine sulfuric acid ester value determined by the fluorescence of a water layer was nearly the same as that from the sulfuric acid ester alone.
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高田
原稿種別: 本文
1960 年19 巻 p.
376-
発行日: 1960年
公開日: 2017/12/22
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高田
原稿種別: 本文
1960 年19 巻 p.
376-
発行日: 1960年
公開日: 2017/12/22
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高田
原稿種別: 本文
1960 年19 巻 p.
376-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
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高田
原稿種別: 本文
1960 年19 巻 p.
376-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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清水
原稿種別: 本文
1960 年19 巻 p.
377-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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高田
原稿種別: 本文
1960 年19 巻 p.
377-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
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川崎 近太郎, 富田 勲
原稿種別: 本文
1960 年19 巻 p.
378-381
発行日: 1960年
公開日: 2017/12/22
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5.6 mg of thiamine sulfuric acid ester was given to rats intraperitoneally or orally and the excretion of thiamine and its sulfuric acid ester in 24 hours' urine was separately estimated by the method proposed by us. The average value in the intraperitoneal dosage was 0.47 mg of thiamine and 4.76 mg of its sulfuric ester, and that in the oral dosage was 0.06 mg of thiamine and 0.38 mg of its sulfuric ester. The value as free thiamine was about one-tenth of its sulfuric ester value : this would be due to the fluorescence of thiochrome sufuric acid ester dissolved into isobutanol. Thiamine sulfuric acid ester was identified by paper partition chromatography in the urine after its BaCl_2-treatment. Thus thiamine sulfuric acid ester was excreted almost completely without its hydrolysis into thiamine on intraperitoneal dosage.
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清水
原稿種別: 本文
1960 年19 巻 p.
381-
発行日: 1960年
公開日: 2017/12/22
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川崎 近太郎, 富田 勲
原稿種別: 本文
1960 年19 巻 p.
382-386
発行日: 1960年
公開日: 2017/12/22
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Thiamine benzenesulfonate in the alkaline medium is instantly transformed into thiamine-anhydride, from which no thiochrome is produced. The mixture of thiamine and its benzenesulfonate is kept alkaline for 5 minutes, then thiamine only can be determined by the usual thiochrome method when it is acidified, while without alkaline treatment the two are determined by the thiochrome method. 7.1 mg of thiamine benzenesulfonate was given to rats intraperitoneally or orally and the excretion of thiamine and its benzenesulfonate in 24 hours' urine was estimated : The average value in intraperitoneal dosage was 0.22 mg of thiamine and 5.88 mg of its benzenesulfonate, and that in oral dosage was 0.07 mg of thiamine and 0.36 mg of its benzenesulfonate. Thiamine benzenesulfonate was identified in the urine by paper partition chromatogrphy, indicating that it was excreted per se in the urine without hydrolysis into thiamine.
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川崎 近太郎, 富田 勲
原稿種別: 本文
1960 年19 巻 p.
387-390
発行日: 1960年
公開日: 2017/12/22
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The daily intraperitoneal injection of 10μg thiamine sulfuric acide ster (I) or 14μg thiamine benzenesulfonate (II) to albino rats on thiamine-deficient diet failed to promote their growth. Later daily dosage was increased to 1OOμg and yet the rats lost their weights. 100μg of II, given orally, however, resulted in a prompt response of weight-gain which suggested hydrolysis of II into thiamine in intestinal tracts. Daily injection of 200μg (I) showed still loss of weight, then an additional daily dosage of 10μg thiamine gave the same response curve as that of thiamine alone. Thus I has been proved to have no anti-thiamine activity to rats.
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清水
原稿種別: 本文
1960 年19 巻 p.
390-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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清水
原稿種別: 本文
1960 年19 巻 p.
390-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
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柳井 哲雄
原稿種別: 本文
1960 年19 巻 p.
391-393
発行日: 1960年
公開日: 2017/12/22
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Thiamine synthesis by washed cells of certain lactic acid bacteria from pyrimidyl- and thiazole moieties of thiamine was investigated. The cell of L. bifidus was found to be a good thiamine synthesizer. Under a good condition, the proportion of synthesized thiamine to the theoretical value was about 100 per cent. For the good yield of the vitamin, it was found that glucose should be added to the reaction mixture, the initial pH should be kept between 5 to 8 inclusive and the cells should be young. L. acidophilus, Lactobacillus from several fermented milk products, and a strain of Streptococcus isolated from a lactic acid bacterial preparation, all failed to synthesize significant amount of thiamine.
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小林 時夫
原稿種別: 本文
1960 年19 巻 p.
394-398
発行日: 1960年
公開日: 2017/12/22
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Paper partition chromatography or paper ionophoresis is hardly applied to determination of a small quantity of FAD in a sample containing large amounts of other flavin derivatives. In such a case, an enzymatic method using D-amino acid oxidase is preferable, so studies were made on this method and following results were obtained. In order to obtain the apo-enzyme which indicated no oxygen uptake without adding FAD, it was necessary to repeat three times the acidification procedure by the method of Warburg. Under the conditions which the author employed, addition of Cu^<++> inhibited the enzyme action. However, other divalent cations such as Zn^<++>, Ni^<++>, Mn^<++>, Mg^<++>, Fe^<++> and Fe^<+++> had no effect. AMP and ATP inhibited the enzyme action, while adenine and adenosine exerted no effect.
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高田
原稿種別: 本文
1960 年19 巻 p.
398-
発行日: 1960年
公開日: 2017/12/22
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上久保
原稿種別: 本文
1960 年19 巻 p.
398-
発行日: 1960年
公開日: 2017/12/22
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小林 時夫
原稿種別: 本文
1960 年19 巻 p.
399-402
発行日: 1960年
公開日: 2017/12/22
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In order to clarify the causes for decrease of the coenzymatic activity of FAD during preservation, studies, were made on the decomposition products by means of paper partition chromatography with several solvent systems. Consequently, two new fluorescent spots were detected when n-butanol・pyridine・water (4 : 6 : 4) was used as the developing solvent. These two substances showed nearly equal Rf values to those of FAD-X and FMN-X respectively in several developing solvents. However, identification of these compounds will be reported in following papers.
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小林 時夫
原稿種別: 本文
1960 年19 巻 p.
402-405
発行日: 1960年
公開日: 2017/12/22
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As reported previously, two decomposition products of FAD, namely Y_1 and Y_2,were detected. Further experiments were performed on some properties of Y_1. The results were as follows : Effect of pH on fluorescence of a solution of the substance and its absorption spectrum were similar to those of FMN. Under an acid hydrolysis of Y_1,FMN was produced and then the fluorescence of solution decreased in some degree. Analysis of Y_1 indicated that the substance contained two moles of phosphate per a flavin moiety. The above results support a supposition that Y_1 may be P^1-(riboflavin-4', 5'-cyclic)-pyrophosphate produced by separation of adenosine moiety from FAD-X.
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高田
原稿種別: 本文
1960 年19 巻 p.
405-
発行日: 1960年
公開日: 2017/12/22
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黒坂 正
原稿種別: 本文
1960 年19 巻 p.
406-412
発行日: 1960年
公開日: 2017/12/22
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For the purpose of ascertaining the role of vitamin A in vision, vitamin A alcohol and ester in retina and pigment layer were estimated by means of alumina chromatography and glyceroldichlorohydrin method. Furthermore the difference of their amounts in the dark and light adaptation was observed. It was assumed that use of 1N KOH・90% ethanol in an atmosphere of nitrogen was desirable for the extraction of vitamin A from retina or pigment layer. Most of vitamin A in retina and pigment layer was found to be alcohol form. In dark adaptation, a decrease of vitamin A alcohol and a slight increase of its ester were observed. On the alumina chromatogram, a yellow layer, differenciated from vitamin A, was found, which was assumed to be a carotenoid compound.
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児玉 貞介, 牧 淳, 本田 一二, 下辻 常介, 向井 清悟, 田村 智英子
原稿種別: 本文
1960 年19 巻 p.
412-416
発行日: 1960年
公開日: 2017/12/22
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It was found by liver function tests and histopathologically that administration of 70 mg/kg of 2-methyl-4-amino-5-hydroxymethylpyrimidine results in liver disturbance in the rabbit. This disturbance is prevented to some extent by vitamin B_6.
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清水
原稿種別: 本文
1960 年19 巻 p.
416-
発行日: 1960年
公開日: 2017/12/22
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山内 和男
原稿種別: 本文
1960 年19 巻 p.
417-429
発行日: 1960年
公開日: 2017/12/22
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In the usual histochemical methed on riboflavin, it was difficult to observe free form and esterified form (FAD or FMN) separately. Moreover, since the fluorescence of FAD is not so much intensive as that of FMN or free riboflavin, measured amounts of FAD are always lower than the true values. Therefore, a trial for improvement of the histochemical method was carried out. Total riboflavin was estimated after hydrolysing the esterified forms by phosphatase, in tissue slice prepared by the author's procedure. Estimation of the esterified form was made after eliminating the free riboflavin by benzyl alcohol. The amount of the free form was calculated from the values of the total and esterified riboflavin. By this improved method, histochemical observation was done on the distribution of riboflavin in various organs of rats. In the small intestine, phosphorylation of riboflavin occurred in Golgi substance and basial mitochondria. FAD formation from free riboflavin or FMN was occurred mainly in the liver. Generally the riboflavin in the living bodies was present in the form of FAD.
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山内 和男
原稿種別: 本文
1960 年19 巻 p.
430-432
発行日: 1960年
公開日: 2017/12/22
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Studies were made on the absorption of riboflavin by histochemical examination of excised intestine of rat, which was kept in Ringer's solution, containing 5 μg/ml of riboflavin, FMN or FAD at 37℃ for 5 to 20 minutes. Free riboflavin was well absorbed in the isolated intestine. When riboflavin passed through the mucous epithelia, a part of it changed into FMN and the remaining part was absorbed in the form of free riboflavin. FMN was absorbed as well as free riboflavin, and it passed through the mucous epithelia in the form of FMN. FAD was not so much absorbed as riboflavin or FMN.
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清水
原稿種別: 本文
1960 年19 巻 p.
432-
発行日: 1960年
公開日: 2017/12/22
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辻 稔, 坂本 司郎, 反田 純郎
原稿種別: 本文
1960 年19 巻 p.
433-437
発行日: 1960年
公開日: 2017/12/22
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Fractional determination of niacinamide and N'-methylniacinamide was successfully carried out by the Kato's method using Amberlite IRA-40O and IRC-50. By these procedures, cationic and anionic fluorescent impurities were completely eliminated. Then a fluorometric determination of niacinamide was made by the alkaline cyanogen bromide method, and of N'-methylniacinamide by the alkaline acetone condensation method. This method was applied to the estimation of the substances in the urine of normal, diabetic, acute hepatic and hyperthyroid patients.
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清水
原稿種別: 本文
1960 年19 巻 p.
437-
発行日: 1960年
公開日: 2017/12/22
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ビタミンB_<12>定量小委員会
原稿種別: 本文
1960 年19 巻 p.
438-445
発行日: 1960年
公開日: 2017/12/22
ジャーナル
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高田
原稿種別: 本文
1960 年19 巻 p.
445-
発行日: 1960年
公開日: 2017/12/22
ジャーナル
フリー
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高田
原稿種別: 本文
1960 年19 巻 p.
445-
発行日: 1960年
公開日: 2017/12/22
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佐橋 佳一, 鈴木 隆雄, 林 淳三, 前川 昭男
原稿種別: 本文
1960 年19 巻 p.
446-
発行日: 1960年
公開日: 2017/12/22
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林 路彰
原稿種別: 本文
1960 年19 巻 p.
446-
発行日: 1960年
公開日: 2017/12/22
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