A basic examination to inspect fruits with non-destructive method by means of nuclear magnetic resonance (NMR) was carried out. The H
2O and D
2O solutions of pure substances such as sugars and acids were used, and effective peaks in the NMR spectra were selected for measuring contents of the substances. Measurements of sugars and acids contents in orange juice extracted were conducted, A nucleus used was H-1, and the apparatus of NMR was 90MHz (2.114T). Results obtained were as follows;
1. Values of chemical shift useful for quantitative analysis of each compound were as follows: saccharose; δ=5.33-5.38, glucose; δ=5.14-5.19, glucose; δ=3.39, citric acid; δ=3.14, malic acid; δ=2.84
2. A peak due to fructose overlapped ones due to other substances. Therefore, an equation for estimation fructose fraction was necessary to be made on the basis of equations to estimate saccharose and glucose, and then peaks due to the combination of these 2 sugars and fructose were developed, using the peaks of which the chemical shift were 3.67-3.90 (B1 peak), 5.33-5.38 (SA peak), and 3.39 (B3 peak), respectively.
3. Accuracy of the estimation with the sweep of 400Hz was improved more than that with the sweep of 800Hz.
4. A big side band was observed near the position of δ=4.7 of chemical shift in the case of solution containing H
2O. It is necessary to change a rotaion rate of sample tube in order to avoid the overlap in this side band.
5. The NMR spectrum of orange juice was most similar to the spectrum of solution mixture of saccharose, fructose, glucose, and citric acid. Other substances is considered to be insensitive to the spectrum.
6. A linear reletionship was obtained between intensity of δ=3.14 peak and concentration of citric acid in fruit juice, and the correlation coefficient was 0.82. The correlation coefficient in the relation sugar (Brix) and estimated values by use of 3 peaks of δ=5.33-5.38, 3.67-3.90, 3.39 was 0.66.
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